{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T23:13:13Z","timestamp":1785366793359,"version":"3.55.0"},"reference-count":28,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,6,2]],"date-time":"2022-06-02T00:00:00Z","timestamp":1654128000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2022,6,2]]},"abstract":"<jats:p>The role of miRNAs as crucial components in carcinogenesis has been well documented. However, whether and how miR-214 influences oral cancer cells\u2019 drug resistance remains to be elucidated, and its downstream targets are still under investigation. Hence, this research is aimed at determining miR-214 and ULK1 expression in oral cancer before and after chemotherapy and their correlations with cancer cell growth. Human oral normal epithelial cells and human tongue squamous cell carcinoma CAL-27 cells were cultured to detect miR-214 and ULK1 levels. It was found that before chemotherapy, miR-214 was higher, while ULK1 was underexpressed in CAL-27 cells, versus normal epithelial cells. After chemotherapy, miR-214 decreased obviously in CAL-27 cells, while ULK1 level increased significantly. In addition, autophagy-related genes (Beclin 1, mTOR, and P53) in CAL-27 cells were found to be significantly inhibited before chemotherapy and were obviously increased after chemotherapy. Moreover, to further determine the impacts of miR-214 and ULK1 on oral cancer cell growth after chemotherapy, the two were overexpressed or silenced in CAL-27 cells after transfection. We found that ULK1 could effectively decrease the activity and invasion of CAL-27 cells and increase their apoptosis level, while miR-214 could antagonize its antitumor effect. Therefore, miR-214 can be used as an early prognostic biomarker for oral cancer, and ULK1 is a new candidate therapeutic target.<\/jats:p>","DOI":"10.1155\/2022\/4589182","type":"journal-article","created":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T01:05:07Z","timestamp":1654218307000},"page":"1-8","source":"Crossref","is-referenced-by-count":4,"title":["miR-214 Modulates the Growth and Migration of Oral Cancer before and after Chemotherapy through Mediating ULK1"],"prefix":"10.1155","volume":"2022","author":[{"given":"Yongtao","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Oral & Maxillofacial Surgery, Shenzhen Stomatology Hospital Affiliated to Shenzhen University, Shenzhen City 518000, GuangDong Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolan","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Stomatology, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen City 518033, GuangDong Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minghua","family":"Li","sequence":"additional","affiliation":[{"name":"Central Laboratory, Peking University Shenzhen Hospital, Shenzhen City 518022, GuangDong Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Limei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Oral & Maxillofacial Surgery, Shenzhen Stomatology Hospital Affiliated to Shenzhen University, Shenzhen City 518000, GuangDong Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Oral & Maxillofacial Surgery, Shenzhen Stomatology Hospital Affiliated to Shenzhen University, Shenzhen City 518000, GuangDong Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5829-4935","authenticated-orcid":true,"given":"Yaomin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Oral & Maxillofacial Surgery, Shenzhen Stomatology Hospital Affiliated to Shenzhen University, Shenzhen University, Shenzhen City 518000, GuangDong Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0528.2012.00704.x"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.7b01402"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1186\/s40880-017-0219-2"},{"key":"4","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1016\/j.cell.2017.07.008","article-title":"Fusobacterium nucleatum promotes chemoresistance to colorectal cancer by modulating autophagy","volume":"170, article e16","author":"T. 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