{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T07:22:59Z","timestamp":1778052179035,"version":"3.51.4"},"reference-count":36,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,1,27]],"date-time":"2022-01-27T00:00:00Z","timestamp":1643241600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Application for scientific research project of Hunan Provincial Health and Family Planning Commission","award":["B2017205"],"award-info":[{"award-number":["B2017205"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Computational and Mathematical Methods in Medicine"],"published-print":{"date-parts":[[2022,1,27]]},"abstract":"<jats:p>Objective. To explore the possibility of microRNA miR-31-3p as a biomarker for bone metastasis of non-small-cell lung cancer (NSCLC) and its molecular mechanism to the invasion and metastasis of NSCLC cells. Methods. Real-time quantitative PCR (RT-qPCR) was used to detect the expression levels of miR-31-3p and forkhead box 1 (FOXO1) in NSCLC tissues, serum, and cells to analyze the correlation between the expression levels of miR-31-3p and the clinicopathology of NSCLC. After interference with or overexpressing miR-31-3p, NSCLC cell proliferation, apoptosis, invasion ability, and migration ability were detected by MTT, flow cytometry, Transwell, and scratch experiment, respectively. The interaction between miR-31-3p and FOXO1 was further verified by the dual-luciferase reporter experiment. Western blot was performed to detect the protein expression of FOXO1 in tissues and FOXO1, RhoA, p-RhoA, ROCK-2, and p-ROCK-2 in cells. Results. In tissues, serum, and NSCLC cell line A549 of the NSCLC patients, the expression of FOXO1 was notably lower, and the miR-31-3p expression was significantly higher. Overexpression of miR-31-3p could distinctly improve the proliferation, invasion, and migration of A549 cells, meanwhile inhibit cell apoptosis, and activate the RhoA\/ROCK-2 signaling pathway, while interfering with the expression of miR-31-3p has the opposite function. Besides, bioinformatics analysis and luciferase reporter assay confirmed that FOXO1 was a target gene of miR-31-3p. Overexpressing FOXO1 could inhibit the proliferation and metastasis of A549 cells, but overexpressing miR-31-3p reverses the results. Conclusion. This study confirmed that miR-31-3p promotes the proliferation, invasion, and migration of NSCLC cells and inhibits apoptosis through targeted regulating FOXO1 and be a potential therapeutic targets for the treatment of NSCLC.<\/jats:p>","DOI":"10.1155\/2022\/4597087","type":"journal-article","created":{"date-parts":[[2022,1,27]],"date-time":"2022-01-27T16:50:09Z","timestamp":1643302209000},"page":"1-11","source":"Crossref","is-referenced-by-count":12,"title":["MiroRNA-31-3p Promotes the Invasion and Metastasis of Non-Small-Cell Lung Cancer Cells by Targeting Forkhead Box 1 (FOXO1)"],"prefix":"10.1155","volume":"2022","author":[{"given":"Xiaoyuan","family":"Zeng","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine, Changsha Central Hospital Affiliated to Nanhua University, Hunan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Da","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, Changsha Central Hospital Affiliated to Nanhua University, Hunan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ganlin","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, Changsha Central Hospital Affiliated to Nanhua University, Hunan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, Changsha Central Hospital Affiliated to Nanhua University, Hunan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4967-3081","authenticated-orcid":true,"given":"Hongzhong","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, Changsha Central Hospital Affiliated to Nanhua University, Hunan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.21037\/tlcr.2018.05.06"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1177\/0300060520939718"},{"key":"3","first-page":"39","article-title":"More than 590,000 people die of lung cancer in China each year","volume":"6","author":"N.C. 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