{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T22:19:31Z","timestamp":1791843571320,"version":"4.3.4"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,4,5]],"date-time":"2022-04-05T00:00:00Z","timestamp":1649116800000},"content-version":"vor","delay-in-days":94,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013494","name":"West Light Foundation, Chinese Academy of Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013494","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002338","name":"Ministry of Education of the People's Republic of China","doi-asserted-by":"publisher","award":["Z2012083"],"award-info":[{"award-number":["Z2012083"]}],"id":[{"id":"10.13039\/501100002338","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81360687"],"award-info":[{"award-number":["81360687"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p><jats:italic>Objective<\/jats:italic>. This study was aimed at investigating the potential mechanism of Grubthobrildkr (GTB) on systemic hypoxia\u2010induced gastric ulcers in rats and at detecting the chemical profile of GTB. <jats:italic>Methods<\/jats:italic>. Male Sprague\u2010Dawley rats were separated into control, hypoxia, hypoxia+omeprazole, and hypoxia+GTBs (0.25, 0.5, and 1.0\u2009g\u00b7kg<jats:sup>-1<\/jats:sup>\u00b7d<jats:sup>-1<\/jats:sup>) groups. Systemic hypoxia was created in a hypobaric chamber to simulate 5000\u2009m high altitude by adjusting the inner pressure and oxygen content for 6 days. After that, the ulcer index, pH, and volume of gastric juice were assessed. The levels of endothelin\u20101 (ET\u20101), gastrin (GAS), motilin (MTL), phospholipase A<jats:sub>2<\/jats:sub> (PLA<jats:sub>2<\/jats:sub>), and prostaglandin E<jats:sub>2<\/jats:sub> (PGE<jats:sub>2<\/jats:sub>) were detected by ELISA. The expression level of hydrogen potassium ATPase (H<jats:sup>+<\/jats:sup>\u2010K<jats:sup>+<\/jats:sup>\u2010ATPase), cyclooxygenase\u20101 (COX\u20101), and cyclooxygenase\u20102 (COX\u20102) was tested by western blotting. Chemical profile of GTB was revealed by UHPLC\u2010Q\u2010exactive hybrid quadrupole\u2010orbitrap mass (UHPLC\u2010Q\u2010Orbitrap MS). <jats:italic>Results<\/jats:italic>. GTB decreased the ulcer index in rats under hypoxia for six days, which was related to increased pH and volume of gastric juice, enhanced MTL and PGE<jats:sub>2<\/jats:sub> levels, and decreased ET\u20101 and PLA<jats:sub>2<\/jats:sub> levels of gastric mucosa. Furthermore, GTB decreased the level of H<jats:sup>+<\/jats:sup>\u2010K<jats:sup>+<\/jats:sup>\u2010ATPase and COX\u20102 while increased COX\u20101 levels in gastric mucosal tissue. 44 constituents were identified by UHPLC\u2010Q\u2010Orbitrap MS in GTB. <jats:italic>Conclusion<\/jats:italic>. GTB exerted a gastroprotective effect to alleviate gastric ulceration induced by acute systemic hypoxia in rats. The effect of GTB increasing the volume and pH of gastric juice in rats under acute systemic hypoxia could be regulated by gastrointestinal hormones, including MTL and ET\u20101. Mechanically, gastrointestinal protection of GTB was based on inhibition of the protons pumping H<jats:sup>+<\/jats:sup>\u2010K<jats:sup>+<\/jats:sup>\u2010ATPase and regulation of prostaglandin family in rats.<\/jats:p>","DOI":"10.1155\/2022\/4803956","type":"journal-article","created":{"date-parts":[[2022,4,5]],"date-time":"2022-04-05T20:05:07Z","timestamp":1649189107000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Mechanism of Traditional Tibetan Medicine Grubthobrildkr Alleviated Gastric Ulcer Induced by Acute Systemic Hypoxia in Rats"],"prefix":"10.1155","volume":"2022","author":[{"given":"Mei","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhanting","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongfang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6482-3255","authenticated-orcid":false,"given":"Zhanqiang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5677-2833","authenticated-orcid":false,"given":"Dianxiang","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4,5]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v13.i5.774"},{"key":"e_1_2_13_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e3181f17adf"},{"key":"e_1_2_13_3_2","first-page":"243","article-title":"Gastric ulcers induced by systemic hypoxia","volume":"43","author":"Syam A. 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