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Leu\u2010Pro\u2010Phe (LPF) is an antioxidative tripeptide isolated from hydrolysates of corn protein. However, the biological activity of LPF <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic> remains unclear. This study is aimed at investigating the protective effects of tripeptide LPF against NTD in the high glucose exposure condition and delineate the underlying biological mechanism. We found that LPF alleviated NTD in the high glucose\u2010exposed chicken embryo model. In addition, DF\u20101 chicken embryo fibroblast was loaded with high glucose for induction of oxidative stress and abnormal <jats:italic>O<\/jats:italic>\u2010GlcNAcylation <jats:italic>in vitro<\/jats:italic>. LPF significantly decreased accumulation of reactive oxygen species and content of malondialdehyde in DF\u20101 cells but increased the ratio of reduced glutathione and oxidized glutathione in chick embryo. Oxygen radical absorbance capacity results showed that LPF itself had good free radical scavenging capacity and could enhance antioxidant activity of the cell content. Mechanistic studies suggested that the resistance of LPF to oxidative damage may be related to promotion of NRF2 expression and nuclear translocation. LPF alleviated the overall <jats:italic>O<\/jats:italic>\u2010GlcNAcylation level of cellular proteins under high glucose conditions and restored the level of Pax3 protein. Collectively, our findings indicate that LPF peptide could act as a nutritional supplement for the protection of development of embryonic neural tube affected by GDM.<\/jats:p>","DOI":"10.1155\/2022\/4932304","type":"journal-article","created":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T01:35:14Z","timestamp":1661823314000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Tripeptide Leu\u2010Pro\u2010Phe from Corn Protein Hydrolysates Attenuates Hyperglycemia\u2010Induced Neural Tube Defect in Chicken 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