{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T20:41:53Z","timestamp":1792442513455,"version":"4.5.0"},"reference-count":27,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,4,21]],"date-time":"2022-04-21T00:00:00Z","timestamp":1650499200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Computational and Mathematical Methods in Medicine"],"published-print":{"date-parts":[[2022,4,21]]},"abstract":"<jats:p>Optic neuritis (ON) is a common neurological disease, and the transplant of retinal ganglion cells (RGCs) has been thought as a promising strategy for improving the injury of the optic nerve system. Bone mesenchymal stem cells (BMSCs) have the potential to differentiate into neural cells. Several studies have indicated that GAP-43 is related with the regeneration of nerve cells, while the effect of GAP-43 on inducing BMSC differentiation remains unclear. In this study, the BMSCs were separated from the rats and identified with flow cytometry assay. The GAP-43 expressed vectors were transfected into the BMSCs, and the biomarkers of RGCs such as PAX6, LHX2, and ATOH7 were used to observe by qRT-PCR. Moreover, the effect of GAP-43-induced BMSCs (G-BMSCs) on ON improvement was also verified with rat models, and the activity of MAPK pathway was measured with western blot. Here, it was found that GAP-43 could obviously promote the differentiation of BMSCs, and increased PAX6, LHX2, ATOH7, BRN3A, and BRN3B were observed in the process of cell differentiation. Moreover, it was also found that G-BMSCs significantly increased the abundances of NFL and NFM in G-BMSCs, and GAP-43 could also enhance the activity of MAPK pathways in BMSCs. Therefore, this study suggested that GAP-43 could induce the differentiation of bone marrow-derived mesenchymal stem cells into retinal ganglial cells.<\/jats:p>","DOI":"10.1155\/2022\/4949206","type":"journal-article","created":{"date-parts":[[2022,4,21]],"date-time":"2022-04-21T14:20:07Z","timestamp":1650550807000},"page":"1-7","source":"Crossref","is-referenced-by-count":3,"title":["GAP-43 Induces the Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells into Retinal Ganglial-Like Cells"],"prefix":"10.1155","volume":"2022","author":[{"given":"Xiaoke","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, The Second Hospital of Jilin University, Changchun, 130000 Jilin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, The First Hospital of Jilin University, Changchun, 130021 Jilin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4710-3894","authenticated-orcid":true,"given":"Lili","family":"Nie","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The Second Hospital of Jilin University, Changchun, 130000 Jilin, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2019.06.001"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1007\/s11910-019-1014-z"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1097\/WCO.0000000000000414"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/2817252"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2021.05.050"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-018-0913-3"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-68745-1"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2016.10.015"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2019.1710916"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1080\/1061186X.2018.1486405"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1219256110"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2016.2532"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2019.107699"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1002\/sctm.16-0428"},{"issue":"1","key":"15","first-page":"277","article-title":"Repairing effect of Schwann cells combined with mesenchymal stem cells on optic nerve injury in rats","volume":"23","author":"M. 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