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There are around fifty species of Legionella, at least twenty\u2010four of which are directly linked to infections in humans. <jats:italic>L. pneumophila<\/jats:italic> is the cause of Legionnaires\u2019 disease, a potentially lethal form of pneumonia. By blocking phagosome\u2010lysosome fusion, <jats:italic>L. pneumophila<\/jats:italic> lives and proliferates inside macrophages. For this disease, there is presently no authorized multiepitope vaccine available. For the multi\u2010epitope\u2010based vaccine (MEBV), the best antigenic candidates were identified using immunoinformatics and subtractive proteomic techniques. Several immunoinformatics methods were utilized to predict B and T cell epitopes from vaccine candidate proteins. To construct an <jats:italic>in silico<\/jats:italic> vaccine, epitopes (07 CTL, 03 HTL, and 07 LBL) were carefully selected and docked with MHC molecules (MHC\u2010I and MHC\u2010II) and human TLR4 molecules. To increase the immunological response, the vaccine was combined with a 50S ribosomal adjuvant. To maximize vaccine protein expression, MEBV was cloned and reverse\u2010translated in <jats:italic>Escherichia coli<\/jats:italic>. To prove the MEBV\u2019s efficacy, more experimental validation is required. After its development, the resulting vaccine is greatly hoped to aid in the prevention of <jats:italic>L. pneumophila<\/jats:italic> infections.<\/jats:p>","DOI":"10.1155\/2022\/4975721","type":"journal-article","created":{"date-parts":[[2022,9,17]],"date-time":"2022-09-17T18:20:06Z","timestamp":1663438806000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Vaccinomics to Design a Multiepitope Vaccine against <i>Legionella pneumophila<\/i>"],"prefix":"10.1155","volume":"2022","author":[{"given":"Ahitsham","family":"Umar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sadia","family":"Liaquat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Israr","family":"Fatima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdur","family":"Rehman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danish","family":"Rasool","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdulrahman","family":"Alshammari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Metab","family":"Alharbi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0137-4584","authenticated-orcid":false,"given":"Muhammad Shahid Riaz","family":"Rajoka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3196-2857","authenticated-orcid":false,"given":"Mohsin","family":"Khurshid","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5495-805X","authenticated-orcid":false,"given":"Usman Ali","family":"Ashfaq","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7810-1026","authenticated-orcid":false,"given":"Asma","family":"Haque","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,9,17]]},"reference":[{"key":"e_1_2_10_1_2","article-title":"Introducing an SPRi-based titration assay using aptamers for the detection of Legionella pneumophila","volume":"351","author":"Saad M.","year":"2021","journal-title":"Sensors and Actuators B: Chemical"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03626.x"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.7.3714-3723.2003"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1094226"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197712012972201"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.3.506-526.2002"},{"key":"e_1_2_10_7_2","unstructured":"Gon\u00e7alvesI. 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