{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T01:26:41Z","timestamp":1788830801037,"version":"build-2803163510"},"reference-count":39,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,3,7]],"date-time":"2022-03-07T00:00:00Z","timestamp":1646611200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16H05840"],"award-info":[{"award-number":["16H05840"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["16H05840"],"award-info":[{"award-number":["16H05840"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004156","name":"Mahidol University","doi-asserted-by":"publisher","award":["16H05840"],"award-info":[{"award-number":["16H05840"]}],"id":[{"id":"10.13039\/501100004156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2022,3,7]]},"abstract":"<jats:p>Objectives. This study is aimed at determining the role of T cells by assessing the numbers of IFN-\u03b3- and IL-2-secreting T cells following stimulation with peptides derived from DNA topoisomerase-I protein in Thai SSc patients. Methods. Fifty Thai SSc patients and 50 healthy controls (HC) joined this study. IFN-\u03b3 and IL-2 levels upon stimulation of T cells with 6 peptides derived from DNA topoisomerase-I protein were determined. Anti-nuclear antibodies (ANA) and anti-Scl-70 antibodies were determined by using the ELISA method. Results. In SSc patients, we detected a significantly higher number of IFN-\u03b3- and IL-2-secreting CD8+ T cells than IFN-\u03b3- and IL-2-secreting CD4+ T cells after stimulation with pooled peptides derived from DNA topoisomerase-I protein. A similar percentage of CD4+IL-2+, CD4+IFN-\u03b3+, and CD8+IL-2+ were detected following stimulation with DNA topoisomerase-I protein -in SSc patients with anti-Scl-70 antibody (SSc\/anti-Scl-70+) and those without. In contrast, the amount of CD8+IFN-\u03b3+ cells was significantly higher in SSc\/anti-Scl-70+ than those without. Stimulation with individual peptides showed that CSLRVEHINLHPELD (sPep3; 15 amino acids; position 505-519 of DNA topoisomerase-I protein) was the optimal epitope that induced T cells secreting the highest levels of IFN-\u03b3 and IL-2. A higher percentage of IFN-\u03b3+CD4+ T cells was detected in SSc\/anti-Scl-70+ than those without the following stimulation with peptides 2 (amino acid position 475-486 [RAVALYFIDKLA] of protein DNA topoisomerase). Conclusion. The results from this study emphasize the critical role of DNA topoisomerase-I peptides on the activation of T cells in SSc patients. The findings provide a better understanding of SSc\u2019s immunopathogenesis and may lead to the development of diagnostic tools and specific treatments for SSc in the future.<\/jats:p>","DOI":"10.1155\/2022\/5072154","type":"journal-article","created":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T03:35:05Z","timestamp":1646710505000},"page":"1-10","source":"Crossref","is-referenced-by-count":2,"title":["Determination of T Cell Responses in Thai Systemic Sclerosis Patients"],"prefix":"10.1155","volume":"2022","author":[{"given":"Oranit","family":"Likhit","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Worawit","family":"Louthrenoo","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sa-nga","family":"Pattanakitsakul","sequence":"additional","affiliation":[{"name":"Division of Molecular Medicine, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aroonroong","family":"Suttitheptumrong","sequence":"additional","affiliation":[{"name":"Division of Molecular Medicine, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Supot","family":"Hannongbua","sequence":"additional","affiliation":[{"name":"The Center of Excellence in Computational Chemistry, Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thanyada","family":"Rungrotmongkol","sequence":"additional","affiliation":[{"name":"Biocatalyst and Environmental Biotechnology Research Unit, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand"},{"name":"Program in Bioinformatics and Computational Biology, Graduate School, Chulalongkorn University, Bangkok 10330, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Noguchi","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Nihon Pharmaceutical University, Saitama 361-0806, Japan"},{"name":"School of Pharmaceutical Science, University of Shizuoka, Shizuoka 422-8526, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fujio","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Science, University of Shizuoka, Shizuoka 422-8526, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7536-8964","authenticated-orcid":true,"given":"Kobporn","family":"Boonnak","sequence":"additional","affiliation":[{"name":"Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/37.2.201"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.17219\/acem\/64334"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200005)43:5<1074::AID-ANR18>3.0.CO;2-E"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/40.10.1135"},{"issue":"2","key":"5","first-page":"202","article-title":"Scleroderma (systemic sclerosis): classification, subsets and pathogenesis","volume":"15","author":"E. 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