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The ADAM9 gene plays a crucial role in the occurrence and development of various liver diseases, but its role in acute alcoholic liver injury remains ambiguous. In this study, a chimeric single\u2010guide RNA targeting the genomic regions of mouse ADAM9 was designed using the clustered regularly interspaced short palindromic repeats (CRISPR)\/CRISPR\u2010associated protein 9 (Cas9) technology. Next, the role of ADAM9 in acute alcoholic liver injury <jats:italic>in vitro<\/jats:italic> in cultured mouse cells and <jats:italic>in vivo<\/jats:italic> in a hydrodynamic injection\u2010based alcoholic liver injury mouse model was documented. The findings of this study suggest that ADAM9 induces by regulating cell proliferation, apoptosis, and stress metabolism in mice. Thus, inhibiting the expression of ADAM9 gene using CRISPR\/Cas9 can attenuate alcohol\u2010induced acute liver injury in mice.<\/jats:p>","DOI":"10.1155\/2022\/5110161","type":"journal-article","created":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T05:35:47Z","timestamp":1654580147000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Silencing the ADAM9 Gene through CRISPR\/Cas9 Protects Mice from Alcohol\u2010Induced Acute Liver 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