{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T02:22:53Z","timestamp":1790130173928,"version":"4.0.1"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,9,6]],"date-time":"2022-09-06T00:00:00Z","timestamp":1662422400000},"content-version":"vor","delay-in-days":248,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016804","name":"Natural Science Foundation of Shenzhen Municipality","doi-asserted-by":"publisher","award":["SUGH2020QD016"],"award-info":[{"award-number":["SUGH2020QD016"]}],"id":[{"id":"10.13039\/100016804","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Contrast Media &amp; Molecular Imaging"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p>In order to evaluate the effects of puerarin combined with poly lactic\u2010co\u2010glycolic acid (PLGA)\/tricalcium phosphate (TCP)\/puerarin on osteocalcin and sialoprotein of mandibular defects, the obtained rat jaw cells are analyzed. The surface morphology of osteoblast complex in the scaffold material group and puerarin combined scaffold material group is observed by a scanning electron microscope, and the growth and proliferation of osteoblasts are detected by the Cell Counting Kit\u20108 (CCK\u20108) method. Besides, the expression of type\u2010I collagen (COL\u2010I), osteocalcin (OC), and osteopontin (OPN) mRNA and related proteins in osteoblasts are detected by immunocytochemical staining. The results of immunocytochemical staining show that puerarin and PLGA\/TCP\/puerarin scaffold had significant effects on the expression of COL\u2010I and OC mRNA and related proteins in osteoblasts. The experimental results indicate that puerarin and PLGA\/TCP\/puerarin can synergistically affect the mRNA and protein expressions of COL\u2010I, OC, and OPN in osteoblasts and have a positive effect on promoting the proliferation activity of osteoblasts.<\/jats:p>","DOI":"10.1155\/2022\/5177419","type":"journal-article","created":{"date-parts":[[2022,9,6]],"date-time":"2022-09-06T20:50:36Z","timestamp":1662497436000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effects of Puerarin Combined with PLGA\/TCP\/Puerarin on Osteocalcin and Sialoprotein of Mandibular Defects"],"prefix":"10.1155","volume":"2022","author":[{"given":"Ying","family":"Guo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianqian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nu","family":"Mi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunmei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuechao","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8053-2629","authenticated-orcid":false,"given":"Hong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,6]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/5152761"},{"key":"e_1_2_10_2_2","first-page":"19","article-title":"Autophagy upregulates inflammatory cytokines in gingival tissue of patient with periodontitis and lipopolysaccharide-stimulated human gingival fibroblasts","volume":"3","author":"Kim W. 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