{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T04:00:50Z","timestamp":1779163250266,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,9,30]],"date-time":"2022-09-30T00:00:00Z","timestamp":1664496000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003550","name":"Queensland Government","doi-asserted-by":"publisher","award":["1180736\/1022752"],"award-info":[{"award-number":["1180736\/1022752"]}],"id":[{"id":"10.13039\/501100003550","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1180736\/1022752"],"award-info":[{"award-number":["1180736\/1022752"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cardiovascular Therapeutics"],"published-print":{"date-parts":[[2022,9,30]]},"abstract":"<jats:p>Background and Aims. The nacht domain, leucine-rich repeat, and pyrin domain-containing protein 3 (NLRP3) inflammasome is upregulated in human abdominal aortic aneurysm (AAA), but its pathogenic role is unclear. The aims of this study were firstly to examine whether the inflammasome was upregulated in a mouse model of AAA and secondly to test whether the inflammasome inhibitor colchicine limited AAA growth. Methods. AAA was induced in eight-week-old male C57BL6\/J mice with topical application of elastase to the infrarenal aorta and oral 3-aminopropionitrile (E-BAPN). For aim one, inflammasome activation, abdominal aortic diameter, and rupture were compared between mice with AAA and sham controls. For aim two, 3 weeks after AAA induction, mice were randomly allocated to receive colchicine (<jats:inline-formula>\n                     <a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                        <a:mi>n<\/a:mi>\n                        <a:mo>=<\/a:mo>\n                        <a:mn>28<\/a:mn>\n                     <\/a:math>\n                  <\/jats:inline-formula>, 0.2\u2009mg\/kg\/d) or vehicle control (<jats:inline-formula>\n                     <c:math xmlns:c=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\">\n                        <c:mi>n<\/c:mi>\n                        <c:mo>=<\/c:mo>\n                        <c:mn>29<\/c:mn>\n                     <\/c:math>\n                  <\/jats:inline-formula>). The primary outcome was the rate of maximum aortic diameter increase measured by ultrasound over 13 weeks. Results. There was upregulation of NLRP3 markers interleukin- (IL-) 1\u03b2 (median, IQR; 15.67, 7.11-22.60\u2009pg\/mg protein versus 6.87, 4.54-11.60\u2009pg\/mg protein, <jats:inline-formula>\n                     <e:math xmlns:e=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\">\n                        <e:mi>p<\/e:mi>\n                        <e:mo>=<\/e:mo>\n                        <e:mn>.048<\/e:mn>\n                     <\/e:math>\n                  <\/jats:inline-formula>) and caspase-1 (109, 83-155 relative luminosity units (RLU) versus 45, 38-65 RLU, <jats:inline-formula>\n                     <g:math xmlns:g=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\">\n                        <g:mi>p<\/g:mi>\n                        <g:mo>&lt;<\/g:mo>\n                        <g:mn>.001<\/g:mn>\n                     <\/g:math>\n                  <\/jats:inline-formula>) in AAA samples compared to controls. Aortic diameter increase over 80 days (mean difference, MD, 4.3\u2009mm, 95% CI 3.3, 5.3, <jats:inline-formula>\n                     <i:math xmlns:i=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\">\n                        <i:mi>p<\/i:mi>\n                        <i:mo>&lt;<\/i:mo>\n                        <i:mn>.001<\/i:mn>\n                     <\/i:math>\n                  <\/jats:inline-formula>) was significantly greater in mice in which aneurysms were induced compared to sham controls. Colchicine did not significantly limit aortic diameter increase over 80 days (MD -0.1\u2009mm, 95% CI -1.1, 0.86, <jats:inline-formula>\n                     <k:math xmlns:k=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\">\n                        <k:mi>p<\/k:mi>\n                        <k:mo>=<\/k:mo>\n                        <k:mn>.922<\/k:mn>\n                     <\/k:math>\n                  <\/jats:inline-formula>). Conclusions. The inflammasome was activated in this mouse model of AAA; however, daily oral administration of colchicine did not limit AAA growth.<\/jats:p>","DOI":"10.1155\/2022\/5299370","type":"journal-article","created":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T02:35:14Z","timestamp":1664591714000},"page":"1-10","source":"Crossref","is-referenced-by-count":13,"title":["Colchicine Does Not Reduce Abdominal Aortic Aneurysm Growth in a Mouse Model"],"prefix":"10.1155","volume":"2022","author":[{"given":"James","family":"Phie","sequence":"first","affiliation":[{"name":"The Vascular Biology Unit, Queensland Research Centre for Peripheral Vascular Disease, James Cook University, Townsville, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0322-6147","authenticated-orcid":true,"given":"Shivshankar","family":"Thanigaimani","sequence":"additional","affiliation":[{"name":"The Vascular Biology Unit, Queensland Research Centre for Peripheral Vascular Disease, James Cook University, Townsville, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pacific","family":"Huynh","sequence":"additional","affiliation":[{"name":"The Vascular Biology Unit, Queensland Research Centre for Peripheral Vascular Disease, James Cook University, Townsville, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raghuveeran","family":"Anbalagan","sequence":"additional","affiliation":[{"name":"The Vascular Biology Unit, Queensland Research Centre for Peripheral Vascular Disease, James Cook University, Townsville, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Corey S.","family":"Moran","sequence":"additional","affiliation":[{"name":"The Vascular Biology Unit, Queensland Research Centre for Peripheral Vascular Disease, James Cook University, Townsville, Queensland, Australia"},{"name":"School of Dentistry, The University of Queensland, Herston Campus, Brisbane, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Kinobe","sequence":"additional","affiliation":[{"name":"College of Public Health, Medical & Veterinary Sciences, James Cook University, Townsville, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph V.","family":"Moxon","sequence":"additional","affiliation":[{"name":"The Vascular Biology Unit, Queensland Research Centre for Peripheral Vascular Disease, James Cook University, Townsville, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matt A.","family":"Field","sequence":"additional","affiliation":[{"name":"Australian Institute of Tropical Health & Medicine and Centre for Tropical Bioinformatics and Molecular Biology, James Cook University, Townsville, Queensland, Australia"},{"name":"Immunogenomics Lab, Garvan Institute of Medical Research, Darlinghurst, NSW, Australia"},{"name":"Menzies School of Health Research, Darwin, NT, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Smriti M.","family":"Krishna","sequence":"additional","affiliation":[{"name":"The Vascular Biology Unit, Queensland Research Centre for Peripheral Vascular Disease, James Cook University, Townsville, Queensland, Australia"},{"name":"Baker Department of Cardiometabolic Health, University of Melbourne, Melbourne, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5779-8848","authenticated-orcid":true,"given":"Jonathan","family":"Golledge","sequence":"additional","affiliation":[{"name":"The Vascular Biology Unit, Queensland Research Centre for Peripheral Vascular Disease, James Cook University, Townsville, Queensland, Australia"},{"name":"Department of Vascular and Endovascular Surgery, Townsville University Hospital, Townsville, Queensland, 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