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The current study analyzed genetic divergence and drug resistance by <jats:italic>S. aureus<\/jats:italic> and therapeutic efficacy of novel antibiotic combinations. The study revealed that 42.30% (minimum 20%\u2010maximum 70%) of milk samples are positive for <jats:italic>S. aureus<\/jats:italic>. Study also revealed seven SNPs in the <jats:italic>S. aureus nuc<\/jats:italic> gene (c.53A&gt;G, c.61A&gt;G, c.73T&gt;C, c.93C&gt;A, c.217C&gt;T, c.280T&gt;C, and c.331T&gt;A). Local isolates Staph\u20102 and Staph\u20103 were closely related to <jats:italic>Bos taurus nuc<\/jats:italic> gene (bovine <jats:italic>S. aureus<\/jats:italic>), while Staph\u20101 was closely related to <jats:italic>Homo sapiens<\/jats:italic> (human <jats:italic>S. aureus<\/jats:italic>) indicating shifting of host. Change of two amino acids and staphylococcal nuclease conserved domain was observed in all local isolates of <jats:italic>S. aureus<\/jats:italic>. The isoelectric points predicted by protParam of Staph\u20101, Staph\u20102, and Staph\u20103 proteins were 9.30, 9.20, and 9.20, respectively. The antibiotic susceptibility profile of <jats:italic>S. aureus<\/jats:italic> presented highest resistance against penicillin (46.67%) and glycopeptide (43.33%). When a single antibiotic regimen was adopted in a field trial, the highest efficacy was reported in the case of oxytetracycline (80%) while lowest was presented by azithromycin. Among antibiotics\u2019 combined regimen, the highest efficacy (80%) was presented by gentamicin with oxytetracycline: cefotaxime with vancomycin; and ciprofloxacin with vancomycin. The current study concluded rising percentages of <jats:italic>S. aureus<\/jats:italic> from dairy milk, proofs of genetic host shifts, and altered responses of in on field therapeutics.<\/jats:p>","DOI":"10.1155\/2022\/5313654","type":"journal-article","created":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T01:50:10Z","timestamp":1655776210000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["[Retracted] Divergent Analyses of Genetic Relatedness and Evidence\u2010Based Assessment of Therapeutics of Staphylococcus aureus from Semi\u2010intensive Dairy Systems"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9610-5429","authenticated-orcid":false,"given":"Sidra","family":"Aziz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1187-0688","authenticated-orcid":false,"given":"Nahla Muhammad","family":"Saeed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9227-4281","authenticated-orcid":false,"given":"Hiewa Othman","family":"Dyary","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7930-7782","authenticated-orcid":false,"given":"Muhammad Muddassir","family":"Ali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7344-6212","authenticated-orcid":false,"given":"Rao Zahid","family":"Abbas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4665-781X","authenticated-orcid":false,"given":"Aziz ur","family":"Rehman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9579-811X","authenticated-orcid":false,"given":"Sammina","family":"Mahmood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3428-4155","authenticated-orcid":false,"given":"Laiba","family":"Shafique","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2579-3038","authenticated-orcid":false,"given":"Zaeem","family":"Sarwar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9748-7059","authenticated-orcid":false,"given":"Fakhara","family":"Khanum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8248-6451","authenticated-orcid":false,"given":"Tean","family":"Zaheer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6140-3909","authenticated-orcid":false,"given":"Khurram","family":"Ashfaq","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8800-2953","authenticated-orcid":false,"given":"Mughees Aizaz","family":"Alvi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8374-8553","authenticated-orcid":false,"given":"Muhammad","family":"Shafeeq","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5176-0403","authenticated-orcid":false,"given":"Arslan","family":"Saleem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7618-3948","authenticated-orcid":false,"given":"Amjad Islam","family":"Aqib","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,6,20]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-019-01922-1"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2009.11.002"},{"key":"e_1_2_9_3_2","volume-title":"The Collected Works of William Morris: With Introductions by His Daughter May Morris","author":"Morris W.","year":"2012"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00015-09"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.12.044"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2015-9587"},{"key":"e_1_2_9_7_2","article-title":"Bovine mastitis as an evolving disease and its impact on the dairy industry","volume":"7","author":"Reshi A. 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