{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T08:13:04Z","timestamp":1769847184359,"version":"3.49.0"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,5,31]],"date-time":"2022-05-31T00:00:00Z","timestamp":1653955200000},"content-version":"vor","delay-in-days":150,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Dean of Scientific Research, Sudan University of Science and Technology, Sudan"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. Cytomegalovirus (CMV) is the most common opportunistic pathogen among renal transplants with significant morbidity and mortality. This study was designed to detect CMV DNA and to determine the frequency of different glycoprotein B (UL55) genotypes among Sudanese renal transplant recipients. <jats:italic>Methods<\/jats:italic>. One hundred and four renal transplant recipients were included in this study. A blood specimen was collected from each recipient. DNA was extracted from plasma using the QIAamp DNA mini kit. CMV amplification and quantification were performed using CMV Real\u2010RT Quant kits. Genotyping of human CMV <jats:italic>gB<\/jats:italic> was carried out by nested PCR and sequencing of the highly diverse region of <jats:italic>gB<\/jats:italic>. <jats:italic>Results<\/jats:italic>. CMV DNA was detected in 40\/104 (38.5%) of renal transplant recipients. The average of the CMV DNA viral load was 358 \u00d7 10<jats:sup>4<\/jats:sup> copies\/ml (6.5 log<jats:sub>10<\/jats:sub>) ranging from 62 copies\/ml (1.8 log<jats:sub>10<\/jats:sub>) to 1.43 \u00d7 10<jats:sup>8<\/jats:sup> copies\/ml (9 log<jats:sub>10<\/jats:sub>). CMV viremia was detected in 60% of recipients of less than 1\u201312 months, 17% of 13\u201324, 10% of 25\u201336, 5% of 37\u201348, and 8% in more than 48 months posttransplantation with no association (<jats:italic>p<\/jats:italic> = 0.296) between CMV viremia and postrenal transplantation time. The association between the type of immunosuppressive drugs and high viral loads (&gt;1000 copies\/ml) showed a significant difference (<jats:italic>p<\/jats:italic> = 0.05). The association between CMV loads of &gt;1000 copies\/ml and symptoms of CMV disease was highly significant (<jats:italic>p<\/jats:italic> \u2264 0.001). Fever 7 (41%), fever and leucopenia 6 (35%), and gastrointestinal disease 4 (24%) were the most common symptoms of CMV disease. CMV genotyping revealed 8 cases (80%) for <jats:italic>gB<\/jats:italic>3 and 2 cases (20%) for <jats:italic>gB<\/jats:italic>4 genotypes. The most frequent genotype among Sudanese renal transplant recipients was <jats:italic>gB<\/jats:italic>3. <jats:italic>Conclusions<\/jats:italic>. The frequency of CMV DNA is high among Sudanese renal transplant recipients. CMV <jats:italic>gB<\/jats:italic>3 is the most predominant glycoprotein B genotype in Sudanese renal transplant recipients.<\/jats:p>","DOI":"10.1155\/2022\/5403694","type":"journal-article","created":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T01:20:14Z","timestamp":1654046414000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Molecular Detection and Glycoprotein B (UL55) Genotyping of Cytomegalovirus among Sudanese Renal Transplant Recipients"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7747-515X","authenticated-orcid":false,"given":"Hind Haidar","family":"Ahmed","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2635-9383","authenticated-orcid":false,"given":"Hisham N.","family":"Altyab","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2588-0636","authenticated-orcid":false,"given":"Samar M.","family":"Saeed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0845-7537","authenticated-orcid":false,"given":"Wafaa Mohammed","family":"Abdalla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfadil Elobeid","family":"Omer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,5,31]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.5272\/jimab.2017234.1834"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2011.04.010"},{"key":"e_1_2_12_3_2","volume-title":"Medical Microbiology","author":"Murray P. 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