{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T13:13:39Z","timestamp":1792502019864,"version":"4.5.0"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,4,15]],"date-time":"2022-04-15T00:00:00Z","timestamp":1649980800000},"content-version":"vor","delay-in-days":104,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p>Extracellular vesicles (EVs) derived from bone marrow mesenchymal stem cells (BMSCs) possess potentials in modulation of the biological process in various diseases. However, an extensive investigation of the mechanism of BMSC\u2010derived EVs (BMSC\u2010EVs) in osteoarthritis (OA) remains unknown. Thus, we focused on the mechanism behind BMSC\u2010EVs in OA. Cartilage tissues were harvested from OA patients, in which the microRNA (miR)\u2010122\u20105p and Sesn2 expression were determined. BMSCs and their EVs were extracted. Chondrocytes were cocultured with BMSC\u2010EVs overexpressing NEAT1, followed by gain\u2010 or loss\u2010of\u2010function assays for studying their effect on cell proliferation, apoptosis, and autophagy. Relationship among NEAT1, miR\u2010122\u20105p, and Sesn2 was assessed. OA mouse model was established by the destabilization of medial meniscus method to elucidate the effect of NEAT1 in vivo. NEAT1 could be transferred from BMSC\u2010EVs into the chondrocytes. miR\u2010122\u20105p was highly expressed but Sesn2 was poorly expressed in cartilage tissues of OA patients. Mechanically, NEAT1 bound to miR\u2010122\u20105p to limit miR\u2010122\u20105p expression which targeted Sesn2, thus activating the Nrf2 pathway. In chondrocytes, NEAT1 delivered by BMSC\u2010EVs, miR\u2010122\u20105p downregulation, or Sesn2 overexpression induced the proliferation and autophagy of chondrocytes but inhibited their apoptosis. Meanwhile, NEAT1 delivered by BMSC\u2010EVs relieved OA by regulating the miR\u2010122\u20105p\/Sesn2\/Nrf2 axis in vivo. Taken altogether, BMSC\u2010EVs containing NEAT1 activated the Sesn2\/Nrf2 axis via binding to miR\u2010122\u20105p for protection against OA.<\/jats:p>","DOI":"10.1155\/2022\/5517648","type":"journal-article","created":{"date-parts":[[2022,4,15]],"date-time":"2022-04-15T21:20:08Z","timestamp":1650057608000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Bone Mesenchymal Stem Cell\u2010Derived Extracellular Vesicles Containing Long Noncoding RNA NEAT1 Relieve Osteoarthritis"],"prefix":"10.1155","volume":"2022","author":[{"given":"Shuai","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7428-7540","authenticated-orcid":false,"given":"Zhe","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4,15]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/boneres.2016.44"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(19)30417-9"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-014-9917-9"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2018.11.001"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cger.2010.03.001"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcna.2019.10.007"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2019.02.036"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-019-1309-7"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevEukaryotGeneExpr.2018023572"},{"key":"e_1_2_10_10_2","article-title":"Extracellular vesicles from mesenchymal stem cells as novel treatments for musculoskeletal diseases","volume":"9","author":"Alcaraz M. 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