{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T21:30:20Z","timestamp":1789075820189,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,4,21]],"date-time":"2022-04-21T00:00:00Z","timestamp":1650499200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Eiken Chemical Co., Ltd."}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Canadian Journal of Gastroenterology and Hepatology"],"published-print":{"date-parts":[[2022,4,21]]},"abstract":"<jats:p>Background. BLEIA \u2122 \u201cEIKEN\u201d Helicobacter pylori antigen (B[EIA]) is based on the bioluminescent enzyme immunoassay (BLEIA) method that was newly developed with high sensitivity in detecting Helicobacter pylori (H. pylori) antigen in feces. Methods. In the project for H. pylori screening and treatment in Saga Prefecture in 2019, 141 students received the stool H. pylori antigen test as a secondary test. For 141 students, a comparative test was conducted between B (EIA) and extracorporeal diagnostic agents that were marketed in Japan as of 2019. The detection performance of H. pylori ATCC43504 standard strain and H. pylori antigen in commercial human fecal specimens were conducted. Results. The comparison of B (EIA) with Quick Chaser TMH. pylori (Q [IC]) revealed positive and negative concordance ratios of B (EIA) to Q (IC) of 100.0% (110\/110) and 71.0% (22\/31), respectively. A comparative test was conducted between B (EIA) and extracorporeal diagnostic agents that were marketed in Japan as of 2019, and B (EIA) was most sensitive on \u201cdetecting H. pylori antigen of ATCC43504 standard strain\u201d and \u201cdetecting H. pylori antigen in commercial human fecal specimens,\u201d compared with other kits. Nine dissociated specimens that were negative for Q (IC) and positive for B (EIA) were confirmed. The measured value of B (EIA) in the dissociation samples were 1.3\u201387.4 cutoff index in the range that can be evaluated as negative by other fecal H. pylori antigen test kits, all the dissociation samples were H. pylori antigen-positive cases, and finally the cause of result divergence was presumed as false negative due to insufficient sensitivity of Q (IC). Conclusion. B (EIA) that is based on the BLEIA method, which applies firefly luciferase luminescence, is more sensitive than stool antigen test kits that are currently marketed in Japan and is very useful in diagnosing H. pylori infection, especially in situations where noninvasive tests are preferred, such as in children.<\/jats:p>","DOI":"10.1155\/2022\/5571542","type":"journal-article","created":{"date-parts":[[2022,4,22]],"date-time":"2022-04-22T03:20:08Z","timestamp":1650597608000},"page":"1-8","source":"Crossref","is-referenced-by-count":8,"title":["Clinical Evaluation of a Novel Stool Antigen Test Using Bioluminescent Enzyme Immunoassay for Detecting Helicobacter pylori"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9995-5522","authenticated-orcid":true,"given":"Toshihiko","family":"Kakiuchi","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Faculty of Medicine, Saga University, Saga 849-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muneaki","family":"Matsuo","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Faculty of Medicine, Saga University, Saga 849-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhisa","family":"Sakata","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Medicine, Saga University, Saga 849-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuma","family":"Fujimoto","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, International University of Health and Welfare, Ohkawa, Fukuoka 831-0016, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19950615)75:12<2789::aid-cncr2820751202>3.0.co;2-4"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1056\/nejmoa001999"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-017-1407-1"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.02.008"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1159\/000339176"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-5378.2011.00889.x"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.l5016"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.g3174"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(08)61159-9"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12146"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1093\/jjco\/hyx128"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-011-0450-6"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1007\/s10120-017-0688-7"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-019-01559-9"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.40.2.649-653.2002"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-200x.2009.02915.x"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1016\/j.gtc.2015.05.008"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1002\/(sici)1522-7243(200001\/02)15:1<21::aid-bio579>3.0.co;2-8"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.58.1170"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1996.0498"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12597"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1007\/s42770-021-00589-x"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01825-18"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-020-04137-7"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfma.2019.12.003"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/7189519"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.3904\/kjim.2016.353"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics11081458"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12184"},{"key":"30","doi-asserted-by":"crossref","DOI":"10.1111\/hel.12696","article-title":"Helicobacter pylori test-and-treat strategy for second-year junior high school students aimed at the prevention of gastric cancer in Takatsuki city","volume":"25","author":"E. Kaji","year":"2020","journal-title":"Helicobacter"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1186\/s12876-020-01549-9"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12396"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(84)91816-6"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.3390\/gastroent12020011"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.080507-0"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1016\/s2468-1253(17)30219-4"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12776"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-004-1398-6"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1111\/ped.13038"},{"issue":"3","key":"40","doi-asserted-by":"crossref","DOI":"10.1111\/hel.12690","article-title":"Effect of probiotics during vonoprazan-containing triple therapy on gut microbiota in Helicobacter pylori infection: a randomized controlled trial","volume":"25","author":"T. Kakiuchi","year":"2020","journal-title":"Helicobacter"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v18.i1.44"},{"issue":"3","key":"42","doi-asserted-by":"crossref","first-page":"225","DOI":"10.7883\/yoken.JJID.2009.225","article-title":"Applicability of a monoclonal antibody-based stool antigen test to evaluate the results of Helicobacter pylori eradication therapy","volume":"62","author":"T. Shimoyama","year":"2009","journal-title":"Japanese Journal of Infectious Diseases"}],"container-title":["Canadian Journal of Gastroenterology and Hepatology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/cjgh\/2022\/5571542.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/cjgh\/2022\/5571542.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/cjgh\/2022\/5571542.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T10:29:23Z","timestamp":1700476163000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/cjgh\/2022\/5571542\/"}},"subtitle":[],"editor":[{"given":"Alessandro","family":"Granito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,4,21]]},"references-count":42,"alternative-id":["5571542","5571542"],"URL":"https:\/\/doi.org\/10.1155\/2022\/5571542","relation":{},"ISSN":["2291-2797","2291-2789"],"issn-type":[{"value":"2291-2797","type":"electronic"},{"value":"2291-2789","type":"print"}],"subject":[],"published":{"date-parts":[[2022,4,21]]}}}