{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T15:57:09Z","timestamp":1787587029573,"version":"build-2736575974"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T00:00:00Z","timestamp":1650844800000},"content-version":"vor","delay-in-days":114,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007225","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","award":["2018 YFC1315100"],"award-info":[{"award-number":["2018 YFC1315100"]}],"id":[{"id":"10.13039\/100007225","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p>\n                    In the present study, we investigated effects of Puerarin on the early oxidative and inflammatory responses in the lung triggered by acute cigarette smoking (ACS). C57BL\/6 mice were exposed to ACS for 1\u2009hr in the presence or absence of Puerarin and harvested at 2, 6, and 24 hours. ACS induced significant increases in superoxide production in mouse lungs at 2 and 6 hours; and superoxide production was also elevated in a time and concentration dependent manner in cigarette smoke extract (CSE) stimulated human small airway epithelial cells (HSAECs), which was dose\u2010dependently abrogated by Puerarin. ACS exposure upregulated NOX1, NOX2, and NOX4 protein expression in mouse lungs. Likewise, NOX1 and NOX4 were upregulated in CSE\u2010stimulated HSAECs. These responses were significantly or completely attenuated by Puerarin. ACS induced significant infiltrations of neutrophils and macrophages in mouse lung parenchyma and BAL fluid, which were completely or significantly abrogated by Puerarin, so was the activation of the NF\u2010\n                    <jats:italic>\u043a<\/jats:italic>\n                    B pathway and the upregulation in inflammatory mediators including TNF\u2010\n                    <jats:italic>\u03b1<\/jats:italic>\n                    , KC (murine homolog of IL\u20108), COX\u20102, IL\u20106 and MCP\u20101. Nuclear translocation of p65, IL\u20108 secretion, and upregulation of COX\u20102 in CSE stimulated HSAECs were also markedly attenuated by Puerarin. Moreover, ACS or CSE stimulated upregulation in reactive oxygen species (ROS) production and expression of inflammatory mediators were alleviated by ROS scavenger TEMPO in vivo and vitro, with no synergy combining with Puerarin, indicating that the effects of Puerarin are redox\u2010sensitive following activation of NOX. In summary, our data for the first time demonstrate that Puerarin robustly attenuates NOX isoform\u2010dependent ROS production and inflammatory activation in ACS exposed mice and CSE treated HSAECs, indicating that Puerarin might be used as a robust therapeutic agent for early or early stage COPD.\n                  <\/jats:p>","DOI":"10.1155\/2022\/5595781","type":"journal-article","created":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T18:20:09Z","timestamp":1650910809000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Reversal of NADPH Oxidase\u2010Dependent Early Oxidative and Inflammatory Responses in Chronic Obstructive Pulmonary Disease by Puerarin"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3536-6032","authenticated-orcid":false,"given":"Pan","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7317-7235","authenticated-orcid":false,"given":"Yixuan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4787-1164","authenticated-orcid":false,"given":"Lu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2743-2319","authenticated-orcid":false,"given":"Xinjing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4896-2930","authenticated-orcid":false,"given":"Shiqing","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7096-8792","authenticated-orcid":false,"given":"Zhenguo","family":"Zhai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7857-5435","authenticated-orcid":false,"given":"Chen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8747-6156","authenticated-orcid":false,"given":"Hua","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4,25]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(17)31222-9"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)31678-6"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(15)60647-X"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.12-2664"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2003.012468"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.87.10.840"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2005.06.021"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000163631.07205.fb"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41569-019-0260-8"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2016.05.011"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2009-0220TR"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.161.2.9811063"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.155.5.9154890"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa032158"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16900-6"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(18)30196-6"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201511-2219OC"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X19500733"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2014.12.002"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2018.03.033"},{"key":"e_1_2_8_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(02)00742-6"},{"key":"e_1_2_8_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4021"},{"key":"e_1_2_8_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2018.10.013"},{"key":"e_1_2_8_24_2","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.19.5.3091"},{"key":"e_1_2_8_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-7-132"},{"key":"e_1_2_8_26_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200202-097OC"},{"key":"e_1_2_8_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2157"},{"key":"e_1_2_8_28_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00008707"},{"key":"e_1_2_8_29_2","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.01448-2018"},{"key":"e_1_2_8_30_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1994.03520190043033"},{"key":"e_1_2_8_31_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1700228"},{"key":"e_1_2_8_32_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201710-2028PP"},{"key":"e_1_2_8_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)30841-9"},{"key":"e_1_2_8_34_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1411532"},{"key":"e_1_2_8_35_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2018.01817"},{"key":"e_1_2_8_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.4423"},{"key":"e_1_2_8_37_2","doi-asserted-by":"publisher","DOI":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