{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:27:04Z","timestamp":1790645224382,"version":"4.1.0"},"reference-count":59,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,3,22]],"date-time":"2022-03-22T00:00:00Z","timestamp":1647907200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Research and Technology of the Republic of Indonesia"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Tropical Medicine"],"published-print":{"date-parts":[[2022,3,22]]},"abstract":"<jats:p>Malaria remains a public health problem and a leading cause of death worldwide. Consequently, the discovery of novel agents, including substances from medicinal plants, is urgently needed. Piper nigrum has long been used by the community in the treatment of the symptoms of malaria. In a previous study, Piper nigrum was demonstrated to exhibit promising antiplasmodial activity against Plasmodium falciparum 3D7 and INDO strains. The aim of this study was to further investigate the antimalarial activity (curative and prophylactic) of piperine (a major isolated constituent of Piper nigrum) in Plasmodium berghei ANKA-infected mice. Piperine 10, 20, and 40\u2009mg\/kg body weight (bw), artesunate 5\u2009mg\/kg bw, and DMSO were administered orally for four days to different groups of Swiss Webster mice. Then, mice were monitored for parasitaemia, body weight, rectal temperature, survival rate, and clinical parameters. Piperine 40\u2009mg\/kg bw in curative and prophylactic tests had the maximum parasitaemia chemosuppression of 79.21% and 58.8% (p\u2009&lt;\u20090.05), respectively, with a significant effect on the survival rate compared with control animals. In the curative test, piperine 40\u2009mg\/kg bw reduced the mean clinical score compared with the control group. Additionally, piperine showed an ability to protect organs (lungs, liver, spleen, and kidneys) from some damage in a dose-dependent manner. This study can be used as a basis for further discovery of novel chemotherapeutic or chemoprophylactic compounds.<\/jats:p>","DOI":"10.1155\/2022\/5721449","type":"journal-article","created":{"date-parts":[[2022,3,22]],"date-time":"2022-03-22T19:50:13Z","timestamp":1647978613000},"page":"1-11","source":"Crossref","is-referenced-by-count":15,"title":["Oral Administration of Piperine as Curative and Prophylaxis Reduces Parasitaemia in Plasmodium berghei ANKA-Infected Mice"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6225-1388","authenticated-orcid":true,"given":"Shafia","family":"Khairani","sequence":"first","affiliation":[{"name":"Doctoral Program, Medical Science, Faculty of Medicine, Padjadjaran University, Bandung, Indonesia"},{"name":"Veterinary Medicine Program, Faculty of Medicine, Padjadjaran University, Bandung, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nisa","family":"Fauziah","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Parasitology Division, Faculty of Medicine, Padjadjaran University, Bandung, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2462-6633","authenticated-orcid":true,"given":"Hesti Lina","family":"Wiraswati","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Parasitology Division, Faculty of Medicine, Padjadjaran University, Bandung, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramdan","family":"Panigoro","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Biochemistry and Molecular Biology Division, Faculty of Medicine, Padjadjaran University, Bandung, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0621-3141","authenticated-orcid":true,"given":"Endang Yuni","family":"Setyowati","sequence":"additional","affiliation":[{"name":"Veterinary Medicine Program, Faculty of Medicine, Padjadjaran University, Bandung, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8809-0810","authenticated-orcid":true,"given":"Afiat","family":"Berbudi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Parasitology Division, Faculty of Medicine, Padjadjaran University, Bandung, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","first-page":"238","article-title":"World malaria report","author":"World Health Organization"},{"issue":"11","key":"2","first-page":"951","article-title":"World malaria report 2021","volume":"6","author":"Autoridad Nacional del Servicio Civil","year":"2021","journal-title":"Angewandte Chemie International Edition"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1002\/med.21486"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.1c00659"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(19)31567-3"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1111\/febs.14127"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1039\/d1nj04198c"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.1c00034"},{"key":"9","volume-title":"Use and Efficacy of Herbal Medicines: Part 1-Historical and Traditional Use","author":"S. 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