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Diabetic cardiomyopathy (DbCM) is the main complication and the cause of high mortality of diabetes. Exploring the transcriptomics and proteomics of DbCM is of great significance for understanding the biology of the disease and for guiding new therapeutic targets for the potential therapeutic effect of spermine (SPM). <jats:italic>Methods and Results<\/jats:italic>. By using a mouse DbCM model, we analyzed the overall transcriptome and proteome of the myocardium, before\/after treatment with SPM. The general state and cardiac structure and function changes of each group were also compared. Diabetes induced an increased blood glucose and serum triglyceride content, a decreased body weight, serum insulin level, and cardiac function\u2010related indexes, accompanied by disrupted myocardial tissue morphology and ultrastructure damage. Using RNA sequencing (RNA\u2010seq), we identified thousands of differentially expressed genes (DEGs) in DbCM with or without SPM treatment. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis demonstrated that the DEGs were significantly enriched in lipid metabolism and amino acid metabolism pathways. Specifically, quantitative real\u2010time PCR (qRT\u2010PCR) confirmed that SPM protected DbCM by reversing the expressions of lipid metabolism and amino acid metabolism\u2010related genes, including Alox15, Gm13033, pla2g12a, Ptges, Pnpla2, and Acot1. To further reveal the pathogenesis of DbCM, we used proteome\u2010based data\u2010independent acquisition (DIA) and identified 139 differentially expressed proteins (DEPs) with 67 being upregulated and 72 being downregulated in DbCM. Venn intersection analysis showed 37 coexpressed genes and proteins in DbCM, including 29 upregulation and 8 downregulation in DbCM. In the protein\u2010protein interaction (PPI) network constructed by the STRING database, the metabolism\u2010related coexpressed genes and proteins, such as Acot2, Ephx2, Cyp1a1, Comt, Acox1, Hadhb, Hmgcs2, Acot1, Inmt, and Cat, can interact with the identified DEGs and DEPs. <jats:italic>Conclusion<\/jats:italic>. The biomarkers and canonical pathways identified in this study may hold the key to understand the mechanisms of DbCM pathobiology and provide new targets for the therapeutic effect of SPM against DbCM by targeting lipid and amino acid metabolism pathways.<\/jats:p>","DOI":"10.1155\/2022\/5909378","type":"journal-article","created":{"date-parts":[[2022,4,9]],"date-time":"2022-04-09T19:50:08Z","timestamp":1649533808000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Transcriptomics Coupled to Proteomics Reveals Novel Targets for the Protective Role of Spermine in Diabetic Cardiomyopathy"],"prefix":"10.1155","volume":"2022","author":[{"given":"Can","family":"Wei","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoxue","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinying","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5936-8430","authenticated-orcid":false,"given":"Ying","family":"Fan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,4,9]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-017-4390-4"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pcad.2019.03.003"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-014-3171-6"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)31320-5"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/1177932218759292"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/bies.200900127"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.1042\/BCJ20160383"},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/iub.230"},{"key":"e_1_2_11_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku837"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2020.101514"},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2017.193"},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.1159\/000438648"},{"key":"e_1_2_11_13_2","doi-asserted-by":"publisher","DOI":"10.1177\/1535370216643417"},{"key":"e_1_2_11_14_2","doi-asserted-by":"publisher","DOI":"10.1159\/000366350"},{"key":"e_1_2_11_15_2","first-page":"85","article-title":"Hypertension in normotensive and hypertensive rats by spermine ingestion","volume":"106","author":"Tipnis U. 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