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To investigate the effect of 3-methyladenine (3-MA) and starvation on the expression of matrix metalloproteinase (MMP-2) in patients with primary open-angle glaucoma. Methods. Primary TM cells were cultured and divided into three groups. The control group was treated with a normal medium, the 3-MA group was stimulated with 3-MA, and the starvation group received nutrient depletion by replacing the normal media with Earle\u2019s balanced salt solution. Cellular mRNA and protein were measured at different 3-MA concentrations and starvation time periods. The level of autophagy was accessed by monodansylcadaverine fluorescent staining and expression of specific autophagy-related genes, light chain 3 (LC3), and Beclin1. The effects of 3-MA and starvation on cell proliferation were determined with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay kit. The mRNA and protein expression of LC3-II, Beclin1, and MMP-2 were measured by reverse transcription-polymerase chain reaction and western blot, respectively. Results. Compared to the control group, starvation significantly upregulated LC3-II and Beclin1 in TM cells after 3\u2009h of stimulation, which peaked at 6\u2009h and 9\u2009h, respectively. Increased MDC-labeled cells were also observed. Starvation downregulated the expression of MMP-2. On the contrary, 3-MA suppressed the activation of autophagy, as shown by the marked downregulation of LC3-II and Beclin1. The expressions of MMP-2 were higher in the 3-MA group compared to the control group, reaching a peak at a concentration of 5\u2009mM. Conclusion. Autophagy may be involved in the pathogenesis of POAG via regulating the expression of MMP-2 and, subsequently, the deposition of the extracellular matrix.<\/jats:p>","DOI":"10.1155\/2022\/6026464","type":"journal-article","created":{"date-parts":[[2022,9,10]],"date-time":"2022-09-10T18:05:09Z","timestamp":1662833109000},"page":"1-9","source":"Crossref","is-referenced-by-count":4,"title":["Autophagy Mediates MMP-2 Expression in Glaucomatous Trabecular Meshwork Cells"],"prefix":"10.1155","volume":"2022","author":[{"given":"Yan-Ling","family":"Xiao","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Rui","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kejing","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wan-Zhu","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing-Ru","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Hong","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5302-547X","authenticated-orcid":true,"given":"Yu-Yu","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)61423-7"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-87844-1"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1089\/jop.2016.0005"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2014.11.021"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2005.06.005"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.07-0984"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.10.026"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.4161\/auto.5.1.7304"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2008.01.013"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2005.10.011"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1002\/bies.950140705"},{"key":"12","first-page":"3","article-title":"The juxtacanalicular region of ocular trabecular meshwork: a tissue with a unique extracellular matrix and specialized function","volume":"1","author":"K. 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