{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T07:25:24Z","timestamp":1767165924144,"version":"build-2238731810"},"reference-count":22,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,2,26]],"date-time":"2022-02-26T00:00:00Z","timestamp":1645833600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81860123"],"award-info":[{"award-number":["81860123"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"First Affiliated Hospital of Nanchang University","award":["81860123"],"award-info":[{"award-number":["81860123"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Disease Markers"],"published-print":{"date-parts":[[2022,2,26]]},"abstract":"<jats:p>Objective. This study focused on investigating the effects of microRNA551b-5p (miR-551b-5p) on severe acute pancreatitis. Methods. Initially, quantitative real-time polymerase chain reaction (qPCR) is employed to determine the expression of miR-551b-5p in differentiated human umbilical vein endothelial cells (HUVECs). Further, the effects of aberrantly expressed miR-551b-5p in HUVECs Transwell assay. The expressions of proteins associated with severe acute pancreatitis capillary leakage syndrome are determined by Western blot, FITC-phalloidin, and immunofluorescence stainings. Finally, the correlative factor and the target genes of miR-551b-5p, as well as their contributions, are assessed. Results. We observed that overexpression of miR-551b-5p distinctly promoted the expression of EGFR, AKT3, and AQP5, while it suppressed the expression of JAM3, AQP1, and occludin. Functionally, the cytoskeleton of the miR-551b-5p overexpression was relatively loose with apparent vacuoles, and overexpression of miR-551b-5p increased the permeability of HUVECs. Conclusion. miR-551b-5p overexpression promoted changes in vascular endothelial permeability via upregulation of the EGFR\/AKT3 pathway and downregulation of occludin and JAM3.<\/jats:p>","DOI":"10.1155\/2022\/6373757","type":"journal-article","created":{"date-parts":[[2022,2,26]],"date-time":"2022-02-26T21:35:08Z","timestamp":1645911308000},"page":"1-6","source":"Crossref","is-referenced-by-count":3,"title":["Study on the Mechanism of MicroRNA551b-5p in Severe Acute Pancreatitis Capillary Leakage Syndrome"],"prefix":"10.1155","volume":"2022","author":[{"given":"Qian","family":"Yao","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua-Jing","family":"Ke","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 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