{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T08:12:54Z","timestamp":1767168774092,"version":"build-2238731810"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,9,10]],"date-time":"2022-09-10T00:00:00Z","timestamp":1662768000000},"content-version":"vor","delay-in-days":252,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Contrast Media &amp; Molecular Imaging"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p>\n                    Most studies have focused on the protective effects of ginkgolide A against ischemia\/reperfusion\u2010induced cardiomyopathy and injury of the brain, liver, and other organs, but there are few reports about the protection of lung tissues. This study was designed to clarify the protection of ginkgolide A against lipopolysaccharide (LPS)\u2010induced pulmonary microvascular endothelial cell (PMVEC) injury. PMVECs were extracted and fell into control, LPS, and ginkgolide A groups. Next, we delved into the growth activity and apoptosis rate of cells\n                    <jats:italic>via<\/jats:italic>\n                    the CCK\u20108 assay and Hoechst staining, independently. Beyond that, western blotting (WB) was implemented for measurement of the expressions of cyclin D1, cyclin\u2010dependent kinase 4 (CDK4), and CDK inhibitor (p21) that pertained to the cell cycle. The target sites of ginkgolide A were confirmed by miRNA array and real\u2010time quantitative PCR. The relationship between miR\u2010224 and p21 was analyzed using dual\u2010luciferase reporter gene assay. Compared with the control group, the LPS group and ginkgolide A group had significantly decreased cell growth activity and relative expressions of cyclin D1 and CDK4 and elevated apoptosis rate and p21 expression. Pronounced elevations were observable in the cell growth activity and expressions of cyclin D1, CDK4, and p21, while the ginkgolide A group presented with a reduced apoptosis rate in comparison with the LPS group (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05). MiR\u2010224 was the target of ginkgolide A, which had targeted regulatory effects on p21. Ginkgolide A can modulate miR\u2010224 expression and regulate p21 expression in a targeted manner to enhance the resistance of PMVECs to LPS\u2010induced cell apoptosis.\n                  <\/jats:p>","DOI":"10.1155\/2022\/6384334","type":"journal-article","created":{"date-parts":[[2022,9,10]],"date-time":"2022-09-10T20:50:05Z","timestamp":1662843005000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["[Retracted] Ginkgolide A Participates in LPS\u2010Induced PMVEC Injury by Regulating miR\u2010224 and Inhibiting p21 in a Targeted Manner"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4206-1605","authenticated-orcid":false,"given":"Zhonglin","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4112-8137","authenticated-orcid":false,"given":"Yan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,9,10]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.purol.2018.06.006"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/ddr.21607"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-18768-2_2"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2020.9097"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.2131\/jts.45.77"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1080\/21655979.2021.2021065"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.26859"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2019.11.101"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.110908"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1691\/ph.2019.9615"},{"key":"e_1_2_8_11_2","first-page":"163","article-title":"[Effect of Ginkgo biloba extract on TLR4\/NF-\u03baB pathway in alleviating cerebral ischemia in mice with acute cerebral ischemia]","volume":"656","author":"Liu L.","year":"2019","journal-title":"Chinese Traditional and Herbal Drugs"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2018.02.009"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcmd.2018.11.003"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14690"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-019-0391-9"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2019.11.167"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.ra119.010903"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/acssensors.9b00927"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00430.2019"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13577-019-00289-7"},{"key":"e_1_2_8_21_2","doi-asserted-by":"publisher","DOI":"10.1097\/shk.0000000000001333"},{"key":"e_1_2_8_22_2","doi-asserted-by":"publisher","DOI":"10.3906\/sag-1804-173"},{"key":"e_1_2_8_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.8b04514"},{"key":"e_1_2_8_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.01.114"},{"key":"e_1_2_8_25_2","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2017.4090"},{"key":"e_1_2_8_26_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00489.2020"},{"key":"e_1_2_8_27_2","doi-asserted-by":"publisher","DOI":"10.1080\/21691401.2019.1623233"}],"updated-by":[{"DOI":"10.1155\/2023\/9825951","type":"retraction","label":"Retraction","source":"retraction-watch","updated":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T00:00:00Z","timestamp":1695772800000},"record-id":"56563"},{"DOI":"10.1155\/2023\/9825951","type":"retraction","label":"Retraction","source":"publisher","updated":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T00:00:00Z","timestamp":1695772800000}}],"container-title":["Contrast Media &amp; Molecular Imaging"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/cmmi\/2022\/6384334.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/cmmi\/2022\/6384334.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2022\/6384334","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T03:14:59Z","timestamp":1721790899000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2022\/6384334"}},"subtitle":[],"editor":[{"given":"Yuvaraja","family":"Teekaraman","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2022,1]]},"references-count":27,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["10.1155\/2022\/6384334"],"URL":"https:\/\/doi.org\/10.1155\/2022\/6384334","archive":["Portico"],"relation":{},"ISSN":["1555-4309","1555-4317"],"issn-type":[{"value":"1555-4309","type":"print"},{"value":"1555-4317","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1]]},"assertion":[{"value":"2022-06-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"6384334"}}