{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:48:40Z","timestamp":1747190920910,"version":"3.40.5"},"reference-count":37,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,2,7]],"date-time":"2022-02-07T00:00:00Z","timestamp":1644192000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institute of Respiratory Diseases Ismael Cos\u00edo Villegas"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Disease Markers"],"published-print":{"date-parts":[[2022,2,7]]},"abstract":"<jats:p>Lung transplantation requires optimization of donor\u2019s organ use through ex vivo lung perfusion (EVLP) to avoid primary graft dysfunction. Biomarkers can aid in organ selection by providing early evidence of suboptimal lungs during EVLP and thus avoid high-risk transplantations. However, predictive biomarkers of pulmonary graft function such as endothelin-converting enzyme (ECE-1) and vascular endothelial growth factor (VEGF) have not been described under EVLP with standard prolonged hypothermic preservation, which are relevant in situations where lung procurement is difficult or far from the transplantation site. Therefore, this study is aimed at quantifying ECE-1 and VEGF, as well as determining their association with hemodynamic, gasometric, and mechanical ventilatory parameters in a swine model of EVLP with standard prolonged hypothermic preservation. Using a protocol with either immediate (I-) or delayed (D-) initiation of EVLP, ECE-1 levels over time were found to remain constant in both study groups (<jats:inline-formula>\n                     <a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                        <a:mi>p<\/a:mi>\n                        <a:mo>&gt;<\/a:mo>\n                        <a:mn>0.05<\/a:mn>\n                     <\/a:math>\n                  <\/jats:inline-formula> RM-ANOVA), while the VEGF protein was higher after prolonged preservation, but it decreased throughout EVLP (<jats:inline-formula>\n                     <c:math xmlns:c=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\">\n                        <c:mi>p<\/c:mi>\n                        <c:mo>&gt;<\/c:mo>\n                        <c:mn>0.05<\/c:mn>\n                     <\/c:math>\n                  <\/jats:inline-formula> RM-ANOVA). Likewise, hemodynamic, gasometric, mechanical ventilatory, and histological parameters had a tendency to better results after 12 hours of hypothermic preservation in the delayed infusion group.<\/jats:p>","DOI":"10.1155\/2022\/6412238","type":"journal-article","created":{"date-parts":[[2022,2,8]],"date-time":"2022-02-08T00:05:15Z","timestamp":1644278715000},"page":"1-8","source":"Crossref","is-referenced-by-count":4,"title":["Endothelin-Converting Enzyme 1 and Vascular Endothelial Growth Factor as Potential Biomarkers during Ex Vivo Lung Perfusion with Prolonged Hypothermic Lung-Sparing"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7974-9078","authenticated-orcid":true,"given":"Claudia","family":"Hern\u00e1ndez-Jim\u00e9nez","sequence":"first","affiliation":[{"name":"Department of Surgical Research, National Institute of Respiratory Diseases Ismael Cos\u00edo Villegas, Mexico City, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2832-1717","authenticated-orcid":true,"given":"J. Ra\u00fal","family":"Olmos-Z\u00fa\u00f1iga","sequence":"additional","affiliation":[{"name":"Experimental Lung Transplant Unit, National Institute of Respiratory Diseases Ismael Cos\u00edo Villegas, Mexico City, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9141-1096","authenticated-orcid":true,"given":"Matilde","family":"Baltazares-Lipp","sequence":"additional","affiliation":[{"name":"Department of Surgical Research, National Institute of Respiratory Diseases Ismael Cos\u00edo Villegas, Mexico City, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8693-1978","authenticated-orcid":true,"given":"Rogelio","family":"Jasso-Victoria","sequence":"additional","affiliation":[{"name":"Department of Surgical Research, National Institute of Respiratory Diseases Ismael Cos\u00edo Villegas, Mexico City, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0139-864X","authenticated-orcid":true,"given":"Adri\u00e1n","family":"Polo-Jerez","sequence":"additional","affiliation":[{"name":"Department of Surgical Research, National Institute of Respiratory Diseases Ismael Cos\u00edo Villegas, Mexico City, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Teresa","family":"P\u00e9rez-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Nursing Research Coordination, National Institute of Respiratory Diseases Ismael Cos\u00edo Villegas, Mexico City, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis Florentino","family":"V\u00e1zquez-Justiniano","sequence":"additional","affiliation":[{"name":"Department of Surgical Research, National Institute of Respiratory Diseases Ismael Cos\u00edo Villegas, Mexico City, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6596-050X","authenticated-orcid":true,"given":"N\u00e9stor Emmanuel","family":"D\u00edaz-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Reprogramming and Tissue Engineering, Department of Medical and Pharmaceutical Biotechnology, Center for Research and Assistance in Technology and Design of the State of Jalisco, A.C, Mexico City, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2945-9793","authenticated-orcid":true,"given":"Miguel","family":"Gaxiola-Gaxiola","sequence":"additional","affiliation":[{"name":"Laboratory of Morphology, National Institute of Respiratory Diseases Ismael Cos\u00edo Villegas, Mexico City, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5158-8129","authenticated-orcid":true,"given":"Laura","family":"Romero-Romero","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Veterinary Medicine and Zootechnics, UNAM, Mexico City, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6575-014X","authenticated-orcid":true,"given":"Axel Edmundo","family":"Guzm\u00e1n-Cedillo","sequence":"additional","affiliation":[{"name":"Department of Surgical Research, National Institute of Respiratory Diseases Ismael Cos\u00edo Villegas, Mexico City, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3547-2142","authenticated-orcid":true,"given":"Mario Enrique","family":"Baltazares-Lipp","sequence":"additional","affiliation":[{"name":"Hemodynamics and Echocardiography Service, National Institute of Respiratory 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