{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T08:15:22Z","timestamp":1767168922985,"version":"build-2238731810"},"reference-count":35,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,4,15]],"date-time":"2022-04-15T00:00:00Z","timestamp":1649980800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Special Scientific Research Projects of Military Health","award":["16BJZ05"],"award-info":[{"award-number":["16BJZ05"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Healthcare Engineering"],"published-print":{"date-parts":[[2022,4,15]]},"abstract":"<jats:p>Objective. The detection of Helicobacter pylori mutations that result in antimicrobial resistance can serve as a guideline of antimicrobial therapeutics and probably prevent the failure of clinical treatments. Evaluating the potential of Sanger sequencing to identify genetically resistant determinants in Helicobacter pylori clinical isolates will be important. Methods. 180 cultured strains have been tested using agar dilution for antibiotic susceptibility. NCBI BLAST was used to perform genotypic analysis on the sequencing data. Sanger sequencing was evaluated as an alternative method to detect resistant genotypes and susceptibility. Results. By the conventional E-test, resistance to levofloxacin, amoxicillin, metronidazole, and clarithromycin was 67.3%, 15.1%, 96.4%, and 25.5%, respectively. In contrast, tetracycline had no resistance. Resistance to multiple drugs was observed in 8.12% of the strains. The genetic determinants of resistance to CLA was 23s rRNA, the determinants of resistance to amoxicillin was Pbp1, the determinants of resistance to metronidazole was rdxA, and the determinants of resistance to levofloxacin were GyrA and GyrB. However, there was no association of resistance in tetracycline. Conclusion. We found increased rates of metronidazole antibiotic resistance, highlighting the necessity for alternative therapies and periodic evaluation. Sanger sequencing has proved to be highly effective and holds the potential to be implemented in policies catering to local treatments.<\/jats:p>","DOI":"10.1155\/2022\/6436256","type":"journal-article","created":{"date-parts":[[2022,4,15]],"date-time":"2022-04-15T19:05:07Z","timestamp":1650049507000},"page":"1-6","source":"Crossref","is-referenced-by-count":9,"title":["Direct Detection of Antibiotic Resistance in Chinese Helicobacter pylori Clinical Isolates by Sequencing-Based Approach"],"prefix":"10.1155","volume":"2022","author":[{"given":"Lixia","family":"Tian","sequence":"first","affiliation":[{"name":"Department of Emergency Medicine, The Eighth Medical Center, Chinese PLA General Hospital, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, The Eighth Medical Center, Chinese PLA General Hospital, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Yin","sequence":"additional","affiliation":[{"name":"Western Medical District of Chinese PLA General Hospital, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lanxiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Xiangshan Road Clinic, The Eighth Medical Center, Chinese PLA General Hospital, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ze","family":"An","sequence":"additional","affiliation":[{"name":"Department of Xiangshan Road Clinic, The Eighth Medical Center, Chinese PLA General Hospital, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7318-3093","authenticated-orcid":true,"given":"Lin","family":"Kang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7857-0404","authenticated-orcid":true,"given":"Chenglin","family":"Ru","sequence":"additional","affiliation":[{"name":"Department of Ultrasound, The Eighth Medical Center, Chinese PLA General Hospital, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4891-922X","authenticated-orcid":true,"given":"Jinping","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Xiangshan Road Clinic, The Eighth Medical Center, Chinese PLA General Hospital, Beijing, China"},{"name":"Department of Gastroenterology, The First Medical Center, Chinese PLA General Hospital, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.1408555"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1111\/evo.13895"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S290835"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1186\/s12941-019-0343-8"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000016254"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2021.121209"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12165"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30753-3"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1097\/mog.0000000000000497"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2018.03.013"},{"issue":"Suppl 6","key":"11","first-page":"487","article-title":"Abstracts from the 3rd international genomic medicine conference (3rd IGMC 2015): jeddah, kingdom of Saudi arabia","volume":"17","year":"2015","journal-title":"BMC Genomics"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2017.11.005"},{"issue":"Suppl 11","key":"13","first-page":"245","article-title":"[Drug sensitivity test for Helicobacter pylori]","volume":"63","author":"K. 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