{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T18:18:39Z","timestamp":1769969919067,"version":"3.49.0"},"reference-count":72,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T00:00:00Z","timestamp":1648512000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2022,3,29]]},"abstract":"<jats:p>Background. Despite modern therapeutic armamentariums, malaria remains a 21st century public health menace. The issue of combating malaria is the ever-growing resistance to high-tech medications in which novel phytomedicines are highly demanding, a rapidly expanding research avenue. In Ethiopian folklore medicine, Urtica simensis has been used to treat malaria by drinking its juice after the dry roots have been mashed and combined with water. Hitherto, no in vivo study has been reported in the literature so far. To substantiate this folkloric claim, the present work herein was done. Methods. An acute oral toxicity study was conducted as per the standard protocol. To rule out, the extract\u2019s inherent potential effects on bodyweight, basal body To, and PCV changes were tracked for two weeks. A four-day suppressive model and a curative assay model were utilized to investigate the antimalarial activity of the plant. Percent parasitemia suppression, packed cell volume, mean survival date, bodyweight, and rectal body temperature were used to determine antimalarial activity. Result. An acute toxicity study reveals that Urtica simensis was atoxic at a dose of 2000\u2009mg\/kg. It also affirms that U. simensis is free from intrinsic potential effects of interfering with bodyweight, temperature, and packed cell volume evolution. Both crude extract and its solvent fractions at all test doses exerted significant (<jats:inline-formula>\n                     <a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                        <a:mi>P<\/a:mi>\n                        <a:mo>&lt;<\/a:mo>\n                        <a:mn>0.001<\/a:mn>\n                     <\/a:math>\n                  <\/jats:inline-formula>) inhibition of parasitemia as compared to the control group. CF400\u2009mg\/kg provided the greatest chemosuppressive effect (79.24%). In a curative experiment, crude extract and CF were able to prevent the cardinal indications of P. berghei-induced malaria, such as weight loss, hypothermia, parasitemia, and anemia. Both crude extracts and their solvent fractions prolong survival dates. Conclusion. The antimalarial activity of the crude extract and its solvent fractions was promising, confirming previous assertions. As a result, more research studies into chemical entities may be required.<\/jats:p>","DOI":"10.1155\/2022\/6702733","type":"journal-article","created":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T19:35:46Z","timestamp":1648582546000},"page":"1-16","source":"Crossref","is-referenced-by-count":4,"title":["Antimalarial Efficacy of Hydromethanolic Root Extract and Solvent Fractions of Urtica simensis Hochst. ex. A. Rich. (Urticaceae): An Experimental Study on Plasmodium berghei-Infected Mice"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2616-4014","authenticated-orcid":true,"given":"Woretaw","family":"Sisay","sequence":"first","affiliation":[{"name":"Department of Pharmacy, College of Health Sciences, Debre Tabor University, Debre Tabor, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4801-4976","authenticated-orcid":true,"given":"Yared","family":"Andargie","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, College of Health Sciences, Debre Tabor University, Debre Tabor, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7904-5823","authenticated-orcid":true,"given":"Mulugeta","family":"Molla","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, College of Health Sciences, Debre Tabor University, Debre Tabor, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/8871375"},{"key":"2","doi-asserted-by":"crossref","DOI":"10.30875\/60123dd4-en","volume-title":"World Malaria Report 2020: 20 Years of Global Progress and Challenges","author":"World Health Organization","year":"2020"},{"key":"3","volume-title":"Malaria","author":"Federal Democratic Republic of Ethiopia","year":"2016"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-021-03866-0"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)00943-0"},{"issue":"2","key":"6","first-page":"127","article-title":"A historical overview of traditional medicine practices and policy in Ethiopia","volume":"20","author":"K. D. Kassaye","year":"2006","journal-title":"Ethiop J Heal Dev"},{"key":"7","volume-title":"Study on Actual Situation of Medicinal Plants in Ethiopia: Prepared for Japan Association for International Collaboration of Agriculture and Forestry","author":"E. Bekele","year":"2007"},{"key":"8","first-page":"1","volume-title":"Non Wood Forest Products: Medicinal Plants for Forest Conservation and Health Care","author":"G. Bodeker","year":"2003"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/4538602"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.3390\/molecules14083037"},{"issue":"147\u2013150","key":"11","article-title":"Evaluation of anti-Plasmodium berghei activity of crude and column fractions of extracts from Withania somnifera","volume":"37","author":"Z. Teklemariam","year":"2013","journal-title":"Turkish Journal of Biology"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.4314\/ejhd.v24i1.62941"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2003.09.028"},{"issue":"35\u201359","key":"14","article-title":"In vitro antimicrobial activity of Iris pseudacorus and Urtica dioica","volume":"16","author":"M. Ramtin","year":"2014","journal-title":"Zahedan J Res Med Sci"},{"key":"15","first-page":"1","article-title":"Ethnobotanical study of medicinal plants used by local communities of Minjar-Shenkora district, North Shewa zone of Amhara region, Ethiopia","volume":"3","author":"K. Alemayehu","year":"2015","journal-title":"J Med Plant Stud"},{"key":"16","first-page":"454","article-title":"Nutritional profile of Samma (Urtica simensis Steudel) leaves grown in Ethiopia","volume":"366","author":"G. A. Eskedar","year":"2013","journal-title":"IJSID"},{"key":"17","doi-asserted-by":"crossref","first-page":"380","DOI":"10.11648\/j.aff.20140305.18","article-title":"Investigating the role of apiculture in watershed management and income improvement in Galessa protected area, Ethiopia","volume":"3","author":"B. K. Tura","year":"2014","journal-title":"Agriculture, Forestry and Fisheries"},{"key":"18","volume-title":"Use and Conservation of Traditional Medicinal Plants in Alelitu Woreda North Shewa","author":"G. S. Negesse","year":"2019"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1186\/s13002-015-0014-6"},{"issue":"1","key":"20","first-page":"24","article-title":"Contribution of traditional farmers for medicinal plant conservation on the farming site in Gozamin District, Amhara Region, Ethiopia","volume":"4","author":"H. Reta","year":"2015","journal-title":"International Journal of Life Sciences"},{"issue":"7","key":"21","first-page":"42","article-title":"An ethnobotanical study of medicinal plants of the Kembatta ethnic group in Enset-based agricultural landscape of Kembatta Tembaro (KT) Zone, Southern Ethiopia","volume":"5","author":"M. Maryo","year":"2015","journal-title":"Asian Journal of Plant Science & Research"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.19026\/crjbs.6.5515"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.15406\/ppij.2019.07.00266"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.4314\/epj.v36i2.3"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.4314\/epj.v27i2.58265"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.2147\/JEP.S270038"},{"key":"27","volume-title":"Transparency and Communication on Animal Research","author":"Declaration B Over I Basel Declaration a Call for More Trust","year":"2010"},{"key":"28","first-page":"5","volume-title":"ICLAS Ethical Guideline for Researchers","author":"International Council for Laboratory Animal Science","year":"2015"},{"key":"29","first-page":"1","volume-title":"Legislation for the Protection of Animals Used for Scientific Purposes","author":"European Union","year":"2010"},{"key":"30","first-page":"269","volume-title":"Methods in Biothechnology: Natural Products Isolation","author":"S. D. Sarker","year":"2006","edition":"Second"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.2147\/JEP.S258586"},{"key":"32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1787\/9789264071049-en","volume-title":"Organization for Economic Cooperation and Development Guidline Test No. 425: Acute Oral Toxicity: Up-And-Down Procedure(UDP)","author":"OECD","year":"2008"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.2147\/JEP.S130491"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.329.7475.1156"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-4758(98)01248-4"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2005.02.017"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.3411"},{"key":"38","first-page":"155","article-title":"The four-day suppressive in vivo antimalarial test","volume":"69","author":"W. Peter","year":"1995","journal-title":"Annals of Tropical Medicine and Parasitology"},{"key":"39","first-page":"209","article-title":"The antimalarial activity of some quinoline esters","volume":"84","author":"J. F. Ryley","year":"1995","journal-title":"Annals of Tropical Medicine and Parasitology"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/8703197"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-016-1529-7"},{"issue":"2","key":"42","first-page":"173","article-title":"Phytochemical screening and anti-oxidant activity of Psidium guajava","volume":"24","author":"N. A. Jani","year":"2020","journal-title":"Malaysian J Anal Sci"},{"issue":"4","key":"43","first-page":"3338","article-title":"Phytochemical screening and qualitative analysis of Cymbopogon citratus","volume":"8","author":"K. Gupta","year":"2019","journal-title":"Journal of Pharmacognosy and Phytochemistry"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.22271\/chemi.2020.v8.i2i.8834"},{"issue":"3","key":"45","first-page":"293","article-title":"Effect of solvents extraction on total phenolics and antioxidant activity of extracts from flaxseed (Linum usitatissimum L.)","volume":"11","author":"F. Anwar","year":"2012","journal-title":"Acta Sci Pol Technol Aliment"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/5384804"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.21315\/mjms2020.27.4.4"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/9749410"},{"key":"49","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.8b01639"},{"key":"50","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/6591070"},{"key":"51","doi-asserted-by":"publisher","DOI":"10.1159\/000058845"},{"key":"52","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/4580526"},{"key":"53","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/7605730"},{"key":"54","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/7347532"},{"key":"55","doi-asserted-by":"publisher","DOI":"10.3390\/molecules22111965"},{"key":"56","doi-asserted-by":"publisher","DOI":"10.1177\/2515690X20984287"},{"key":"57","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2020.112694"},{"key":"58","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/8846067"},{"key":"59","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/9932891"},{"key":"60","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/6165950"},{"key":"61","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/8324596"},{"key":"62","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/2089114"},{"key":"63","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/8833865"},{"key":"64","doi-asserted-by":"publisher","DOI":"10.3906\/biy-1203-62"},{"key":"65","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/8832724"},{"key":"66","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/8980212"},{"key":"67","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/6651922"},{"key":"68","doi-asserted-by":"publisher","DOI":"10.13057\/asianjethnobiol\/y040105"},{"issue":"5","key":"69","first-page":"204","article-title":"In vivo antimalarial activity of solvent fractions of Echinops kebericho roots against Plasmodium berghei infected mice","volume":"12","author":"A. Toma","year":"2017","journal-title":"EC Microbiol"},{"key":"70","doi-asserted-by":"publisher","DOI":"10.52292\/j.laar.2017.295"},{"key":"71","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/6674002"},{"key":"72","doi-asserted-by":"publisher","DOI":"10.21203\/rs.2.19327\/v1"}],"container-title":["Evidence-Based Complementary and Alternative Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2022\/6702733.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2022\/6702733.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2022\/6702733.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T19:36:12Z","timestamp":1648582572000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/ecam\/2022\/6702733\/"}},"subtitle":[],"editor":[{"given":"Abimbola","family":"Oluyori","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2022,3,29]]},"references-count":72,"alternative-id":["6702733","6702733"],"URL":"https:\/\/doi.org\/10.1155\/2022\/6702733","relation":{},"ISSN":["1741-4288","1741-427X"],"issn-type":[{"value":"1741-4288","type":"electronic"},{"value":"1741-427X","type":"print"}],"subject":[],"published":{"date-parts":[[2022,3,29]]}}}