{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T22:43:49Z","timestamp":1793227429632,"version":"4.5.0"},"reference-count":42,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,4,14]],"date-time":"2022-04-14T00:00:00Z","timestamp":1649894400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2022,4,14]]},"abstract":"<jats:p>Introduction. Natural products are among the most useful sources for the discovery of new drugs against various diseases. Keeping in view the ethnobotanical relevance ethnopharmacological significance of Polygonaceae family in diabetes, the current study was designed to isolate pure compounds from Persicaria hydropiper L. leaves and evaluate their in vitro and in silico antidiabetic potentials. Methods. Six compounds were isolated from the chloroform-ethyl acetate fractions using gravity column chromatography and were subjected to structure elucidation process. Structures were confirmed using 1H-NMR, 13C-NMR, and mass spectrometry techniques. Isolated phytochemicals were subjected to in vitro antidiabetic studies, including \u03b1-glucosidase, \u03b1-amylase inhibition, and DPPH, and ABTS antioxidant studies. Furthermore, the in silico binding mode of these compounds in the target enzymes was elucidated via MOE-Dock software. Results. The isolated compounds revealed concentration-dependent inhibitions against \u03b1-glucosidase enzyme. Ph-1 and Ph-2 were most potent with 81.84 and 78.79% enzyme inhibitions at 1000\u2009\u00b5g\u00b7mL\u22121, respectively. Ph-1 and Ph-2 exhibited IC50s of 85 and 170\u2009\u00b5g\u00b7mL\u22121 correspondingly. Likewise, test compounds showed considerable \u03b1-amylase inhibitions with Ph-1 and Ph-2 being the most potent. Tested compounds exhibited considerable antioxidant potentials in both DPPH and ABTS assays. Molecular simulation studies also revealed top-ranked confirmations for the majority of the compounds in the target enzymes. Highest observed potent compound was Ph-1 with docking score of \u221212.4286 and formed eight hydrogen bonds and three H-pi linkages with the Asp 68, Phe 157, Phe 177, Asn 241, Glu 276, His 279, Phe 300, Glu 304, Ser 308, Pro 309, Phe 310, Asp 349, and Arg 439 residues of \u03b1-glucosidase binding packets. Asp 68, Glu 276, Asp 349, and Arg 439 formed polar bonds with the 3-ethyl-2-methylpentane moiety of the ligand. Conclusions. The isolated compounds exhibited considerable antioxidant and inhibitory potentials against vital enzymes implicated in T2DM. The docking scores of the compounds revealed that they exhibit affinity for binding with target ligands. The enzyme inhibition and antioxidant potential of the compounds might contribute to the hypoglycemic effects of the plant and need further studies.<\/jats:p>","DOI":"10.1155\/2022\/6705810","type":"journal-article","created":{"date-parts":[[2022,4,14]],"date-time":"2022-04-14T18:20:09Z","timestamp":1649960409000},"page":"1-13","source":"Crossref","is-referenced-by-count":15,"title":["Antioxidant, Enzyme Inhibitory, and Molecular Docking Approaches to the Antidiabetic Potentials of Bioactive Compounds from Persicaria hydropiper L."],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4299-2445","authenticated-orcid":true,"given":"Muhammad","family":"Ayaz","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Faculty of Biological Sciences, University of Malakand, Chakdara 18000, Dir (L), KP, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0154-7863","authenticated-orcid":true,"given":"Abdul","family":"Sadiq","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Biological Sciences, University of Malakand, Chakdara 18000, Dir (L), KP, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5052-7116","authenticated-orcid":true,"given":"Osama F.","family":"Mosa","sequence":"additional","affiliation":[{"name":"Public Health Department Health Sciences College at Lieth, Umm Al Qura University, Makkah, Saudi Arabia"},{"name":"Biochemistry Department, Bukhara State Medical Institute Named after Abu Ali Ibn Sino, Bukhara, Uzbekistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tariq Abdalla","family":"Zafar","sequence":"additional","affiliation":[{"name":"Public Health Department Health Sciences College at Lieth, Umm Al Qura University, Makkah, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9068-3818","authenticated-orcid":true,"given":"Alashary Adam","family":"Eisa Hamdoon","sequence":"additional","affiliation":[{"name":"Public Health Department Health Sciences College at Lieth, Umm Al Qura University, Makkah, Saudi Arabia"},{"name":"Faculty of Public and Environmental Health, University of Khartoum, Khartoum, Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9274-7026","authenticated-orcid":true,"given":"Modawy Elnour Modawy","family":"Elkhalifa","sequence":"additional","affiliation":[{"name":"Public Health Department Health Sciences College at Lieth, Umm Al Qura University, Makkah, Saudi Arabia"},{"name":"Faculty of Public and Environmental Health, University of Khartoum, Khartoum, Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6060-5533","authenticated-orcid":true,"given":"Mohammed Ahmed","family":"Elawad","sequence":"additional","affiliation":[{"name":"Public Health Department Health Sciences College at Lieth, Umm Al Qura University, Makkah, Saudi Arabia"},{"name":"Faculty of Public and Environmental Health, University of Khartoum, Khartoum, Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8083-9145","authenticated-orcid":true,"given":"Alshebli","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Public Health Department Health Sciences College at Lieth, Umm Al Qura University, Makkah, Saudi Arabia"},{"name":"Faculty of Public and Environmental Health, University of Khartoum, Khartoum, Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7907-0842","authenticated-orcid":true,"given":"Farhat","family":"Ullah","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Biological Sciences, University of Malakand, Chakdara 18000, Dir (L), KP, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1620-8610","authenticated-orcid":true,"given":"Mehreen","family":"Ghufran","sequence":"additional","affiliation":[{"name":"Department of Pathology, MTI Bacha Khan Medical College, Mardan, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2890-1575","authenticated-orcid":true,"given":"Atul","family":"Kabra","sequence":"additional","affiliation":[{"name":"University Institute of Pharma Sciences, Chandigarh University, Gharuan, Mohali-140413, Punjab, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2020.558641"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.21.9.1414"},{"issue":"1","key":"3","first-page":"128","article-title":"In vitro studies on alpha amylase and alpha glucosidase inhibitory activities of selected plant extracts","volume":"3","author":"S. 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