{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T08:35:58Z","timestamp":1779266158086,"version":"3.51.4"},"reference-count":61,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,12,22]],"date-time":"2022-12-22T00:00:00Z","timestamp":1671667200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100015027","name":"Vice Chancellor for Research and Technology, Hamadan University of Medical Sciences","doi-asserted-by":"publisher","award":["9812139500"],"award-info":[{"award-number":["9812139500"]}],"id":[{"id":"10.13039\/501100015027","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Inflammation"],"published-print":{"date-parts":[[2022,12,22]]},"abstract":"<jats:p>Objective. Pulmonary toxicity induced by CCl4, a model of idiopathic pulmonary fibrosis (IPF), leads to tissue remodeling and inflammation. Human umbilical cord mesenchymal cell-conditioned medium (hMSC-CM) is a potent anti-inflammatory, antioxidative, and antifibrotic agent. Methods. Forty male Wistar rats were assigned to the control (C), olive oil control (C.O) (hMSC-CM), control (C.Ms), fibrosis (fb), and fibrosis with hMSC-CM (f.Ms) treatment groups. The groups C, C.O, and C.Ms received PBS (200\u2009\u00b5l), olive oil (1\u2009ml\/kg), and hMSC-CM (100\u2009\u03bcg protein\/kg), respectively. The fibrosis group was administered with only CCl4 (1\u2009ml\/kg). The last group, f.Ms was treated with CCl4 (1\u2009ml\/kg) and 100\u2009\u03bcg protein\/kg IV hMSC-CM. While the treatment with olive oil and CCl4 was performed for 2 days\/week from the first week for 12 weeks, the treatment with PBS and hMSC-CM was carried out 2 days\/week from week 4th to week 12th. The effect of the UC-MSC culture medium treatment on the lung was evaluated by assessing lysyl oxidase (LOX), tumor necrosis factor-alpha (TNF-\u03b1), and transforming growth factor-\u03b21 (TGF-\u03b21) genes, and proteins expression by real-time RCR and western blotting, respectively. Results. Lysyl oxidase (LOX), tumor necrosis factor-alpha (TNF-\u03b1), transforming growth factor-b1 (TGF-\u03b21), malondialdehyde (MDA), and oxidative stress levels were markedly higher in the fibrosis group than in the control groups (<jats:inline-formula>\n                     <a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                        <a:mi>p<\/a:mi>\n                        <a:mo>\u2264<\/a:mo>\n                        <a:mn>0.001<\/a:mn>\n                     <\/a:math>\n                  <\/jats:inline-formula>). Additionally, glutathione (GSH) in the fibrosis group was markedly lower than those in the control groups (<jats:inline-formula>\n                     <c:math xmlns:c=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\">\n                        <c:mi>p<\/c:mi>\n                        <c:mo>\u2264<\/c:mo>\n                        <c:mn>0.001<\/c:mn>\n                     <\/c:math>\n                  <\/jats:inline-formula>). Fibrosis in the UC-MSC treatment group had milder histopathological injuries than in the fibrosis group. Conclusion. hMSC-MSC as a strong anti-inflammatory, antioxidative, and antifibrotic decreases the level of oxidative stress, proinflammatory cytokines, and MDA causing a restoring effect against CCl4-induced pulmonary fibrosis.<\/jats:p>","DOI":"10.1155\/2022\/7179766","type":"journal-article","created":{"date-parts":[[2022,12,22]],"date-time":"2022-12-22T21:50:07Z","timestamp":1671745807000},"page":"1-10","source":"Crossref","is-referenced-by-count":4,"title":["The Restoring Effect of Human Umbilical Cord-Derived Mesenchymal Cell-Conditioned Medium (hMSC-CM) against Carbon Tetrachloride-Induced Pulmonary Fibrosis in Male Wistar Rats"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6344-7289","authenticated-orcid":true,"given":"Maryam","family":"Khajvand-Abedini","sequence":"first","affiliation":[{"name":"Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2762-0958","authenticated-orcid":true,"given":"Mahdi","family":"Bahmani","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7473-9823","authenticated-orcid":true,"given":"Nasrin","family":"Ziamajidi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3567-8677","authenticated-orcid":true,"given":"Alireza","family":"Nourian","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Veterinary Medicine Clinical Biochemistry, University of Bu-Ali Sina, Hamadan, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5311-8630","authenticated-orcid":true,"given":"Parisa","family":"Habibi","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4425-1074","authenticated-orcid":true,"given":"Shirin","family":"Heidarisasan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6345-1410","authenticated-orcid":true,"given":"Roghayeh","family":"Abbasalipourkabir","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201312-2221pp"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1093\/jmcb\/mju039"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1007\/s13770-016-0007-0"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1177\/0363546509348047"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1002\/term.214"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v21.i18.5473"},{"issue":"6","key":"7","first-page":"685","article-title":"The protective effect of infliximab against carbon tetrachloride-induced acute lung injury","volume":"19","author":"A. 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