{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T15:42:23Z","timestamp":1784043743620,"version":"3.55.0"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,4,30]],"date-time":"2022-04-30T00:00:00Z","timestamp":1651276800000},"content-version":"vor","delay-in-days":119,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p><jats:italic>Staphylococcus aureus<\/jats:italic> is a major human pathogen that is sometimes resistant to vancomycin. In this study, the prevalence of vancomycin\u2010resistant <jats:italic>Staphylococcus aureus<\/jats:italic> (VRSA) was studied. 100 isolates of <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic> were identified based on biochemical and molecular evidence. The antibiotic susceptibility of the studied isolates was tested against 13 antibiotics by the disc diffusion method that showed 24 vancomycin\u2010resistant isolates. The minimum inhibitory concentrations (MICs) were estimated by the agar dilution method to determine vancomycin intermediate\u2010resistant <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic> (VISA) and VRSA. The resistance gene cluster (<jats:italic>vanA<\/jats:italic>, <jats:italic>vanR<\/jats:italic>, <jats:italic>vanH<\/jats:italic>, and <jats:italic>vanY<\/jats:italic>) was amplified by PCR and then sequenced. Amplification of <jats:italic>vanA<\/jats:italic> and <jats:italic>vanR<\/jats:italic> genes showed that they are present in 21.4% and 14.3% of VRSA isolates, respectively, whereas none of the studied genes has been detected in VISA strains. A significant antimicrobial effect toward VRSA isolates using silver nanoparticles (AgNPs) synthesized from <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic> and rosemary leaves was recorded. This study confirmed the existence of VRSA strains in Egypt. Furthermore, the use of silver nanoparticles inhibits these vancomycin\u2010resistant <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic> strains <jats:italic>in vitro<\/jats:italic>.<\/jats:p>","DOI":"10.1155\/2022\/7380147","type":"journal-article","created":{"date-parts":[[2022,4,30]],"date-time":"2022-04-30T22:35:07Z","timestamp":1651358107000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Inhibition of the Vancomycin Resistance in <i>Staphylococcus aureus<\/i> in Egypt Using Silver Nanoparticles"],"prefix":"10.1155","volume":"2022","author":[{"given":"Nouran M.","family":"Salah","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal E.","family":"Saafan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman H.","family":"Salem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4347-6864","authenticated-orcid":false,"given":"Haddad A.","family":"El Rabey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed A.","family":"Alsieni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuad A.","family":"Alatawi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adel I.","family":"Alalawy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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