{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T15:34:54Z","timestamp":1764084894691,"version":"3.40.5"},"reference-count":28,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,7,15]],"date-time":"2022-07-15T00:00:00Z","timestamp":1657843200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Construction of Information Follow-up Management Platform for Accurate Diagnosis and Treatment of Common and Difficult Skin Diseases in Xinjiang","award":["202109736"],"award-info":[{"award-number":["202109736"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2022,7,15]]},"abstract":"<jats:p>This work was to investigate mechanism by which mir-22 targeting nod-like receptor protein 3 (NLRP3) inflammasome affected activity of human skin malignant melanoma (MM) A375 cells. Twenty-four mice were rolled into a control group (Group X) and an experimental group (Group Y) randomly. Without treatment in Group X, Group Y established MM model. After cell transfection, the mice were divided into group A (blank group), group B (negative group), group C (miR-22 mimics group), group D (miR-22 inhibitor group), and group E (miR-22 inhibitor+siNLRP3 group). The results were summarized as follows. The level of miR-22 mRNA in Group Y was obviously lower than that in Group X, and levels of NLRP3 and caspase-1 mRNA and NLRP3 and caspase-1 protein in Group Y were greatly higher than those in Group X (<jats:inline-formula>\n                     <a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                        <a:mi>P<\/a:mi>\n                        <a:mo>&lt;<\/a:mo>\n                        <a:mn>0.05<\/a:mn>\n                     <\/a:math>\n                  <\/jats:inline-formula>). The mRNA levels of miR-22 mRNA in group C were much higher in contrast to those in group A, and the mRNA levels of NLRP3 and caspase-1 were lower. The contrast results in group D and group A were the opposite, <jats:inline-formula>\n                     <c:math xmlns:c=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\">\n                        <c:mi>P<\/c:mi>\n                        <c:mo>&lt;<\/c:mo>\n                        <c:mn>0.05<\/c:mn>\n                     <\/c:math>\n                  <\/jats:inline-formula>. The levels of NLRP3 and caspase-1 proteins in group C were greatly elevated, and those in group D were decreased compared with those in group A (<jats:inline-formula>\n                     <e:math xmlns:e=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\">\n                        <e:mi>P<\/e:mi>\n                        <e:mo>&lt;<\/e:mo>\n                        <e:mn>0.05<\/e:mn>\n                     <\/e:math>\n                  <\/jats:inline-formula>). Therefore, miR-22 may target and inhibit the activation of the NLRP3 inflammasome to reduce the activity of cutaneous malignant melanoma A375 cells.<\/jats:p>","DOI":"10.1155\/2022\/7420330","type":"journal-article","created":{"date-parts":[[2022,7,16]],"date-time":"2022-07-16T03:50:11Z","timestamp":1657943411000},"page":"1-8","source":"Crossref","is-referenced-by-count":3,"title":["Influencing Mechanism of Nod-Like Receptor Protein 3 Inflammasome Activation in A375 Cell Activity in Human Cutaneous Malignant Melanoma"],"prefix":"10.1155","volume":"2022","author":[{"given":"Akebaier","family":"Sulaiman","sequence":"first","affiliation":[{"name":"Department of Dermatology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uyghur Autonomous Region, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Dermatology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uyghur Autonomous Region, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junwei","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Dermatology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uyghur Autonomous Region, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reyila","family":"Abuliezi","sequence":"additional","affiliation":[{"name":"Department of Dermatology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uyghur Autonomous Region, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Dermatology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uyghur Autonomous Region, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4027-9897","authenticated-orcid":true,"given":"Xuefeng","family":"Wan","sequence":"additional","affiliation":[{"name":"Department of Dermatology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uyghur Autonomous Region, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"issue":"3","key":"1","doi-asserted-by":"crossref","first-page":"292","DOI":"10.4103\/ijc.IJC_612_17","article-title":"Clinicopathological study of malignant melanoma in a regional cancer center","volume":"55","author":"S. Panda","year":"2018","journal-title":"Indian Journal of Cancer"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1007\/s12325-021-01917-1"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.32471\/exp-oncology.2312-8852.vol-42-no-4.15334"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1038\/s41374-019-0358-y"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1111\/ijd.14818.Epub"},{"issue":"11, article 604179","key":"6","article-title":"The gut-brain axis: how microbiota and host inflammasome influence brain physiology and pathology","volume":"10","author":"A. Rutsch","year":"2020","journal-title":"Frontiers in Immunology"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2020.07.016"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1295-z"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2018100376"},{"key":"10","first-page":"8151836","article-title":"Lipopolysaccharide (LPS) aggravates high glucose- and hypoxia\/reoxygenation-induced injury through activating ROS-dependent NLRP3 inflammasome-mediated pyroptosis in H9C2 cardiomyocytes","volume":"2019","author":"Z. 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Ralli","year":"2020","journal-title":"Journal of Immunology Research"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.1001.Epub"},{"issue":"10","key":"21","doi-asserted-by":"crossref","first-page":"990","DOI":"10.3389\/fimmu.2019.00990","article-title":"Combination of immunotherapy with targeted therapy: theory and practice in metastatic melanoma","volume":"7","author":"C. Yu","year":"2019","journal-title":"Frontiers in Immunology"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1111\/pcmr.12591.Epub"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18081647"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1002\/JLB.3MR0720-472RR.Epub"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.3390\/biom9070275.Retraction"},{"issue":"1","key":"26","first-page":"218","article-title":"Anticancer effects of kaempferol in A375 human malignant melanoma cells are mediated via induction of apoptosis, cell cycle arrest, inhibition of cell migration and downregulation of m-TOR\/PI3K\/AKT pathway","volume":"23","author":"J. Yang","year":"2018","journal-title":"Journal of BUON"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-89792-2"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2021.102763.Epub"}],"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2022\/7420330.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2022\/7420330.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2022\/7420330.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,16]],"date-time":"2022-07-16T03:50:19Z","timestamp":1657943419000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/jir\/2022\/7420330\/"}},"subtitle":[],"editor":[{"given":"Fu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2022,7,15]]},"references-count":28,"alternative-id":["7420330","7420330"],"URL":"https:\/\/doi.org\/10.1155\/2022\/7420330","relation":{},"ISSN":["2314-7156","2314-8861"],"issn-type":[{"type":"electronic","value":"2314-7156"},{"type":"print","value":"2314-8861"}],"subject":[],"published":{"date-parts":[[2022,7,15]]}}}