{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T06:40:57Z","timestamp":1792651257613,"version":"4.5.0"},"reference-count":53,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T00:00:00Z","timestamp":1655769600000},"content-version":"vor","delay-in-days":171,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018557","name":"Science and Technology Project of Nantong City","doi-asserted-by":"publisher","award":["MS12021014"],"award-info":[{"award-number":["MS12021014"]}],"id":[{"id":"10.13039\/501100018557","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81703099"],"award-info":[{"award-number":["81703099"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p>Keloid is a common dermatofibrotic disease with excessive skin fibroblast proliferation. Hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) as the third gasotransmitter improves fibrosis of various organs and tissues. Our study is aimed at investigating the effects and possible mechanisms of H<jats:sub>2<\/jats:sub>S on skin fibroblast proliferation. Scar tissues from six patients with keloid and discarded skin tissue from six normal control patients were collected after surgery, respectively. Plasma H<jats:sub>2<\/jats:sub>S content and skin H<jats:sub>2<\/jats:sub>S production in patients with keloid were measured. Keloid fibroblasts and transforming growth factor\u2010<jats:italic>\u03b2<\/jats:italic><jats:sub>1<\/jats:sub>\u2010 (TGF\u2010<jats:italic>\u03b2<\/jats:italic><jats:sub>1<\/jats:sub>, 10\u2009ng\/mL) stimulated normal skin fibroblasts were pretreated with H<jats:sub>2<\/jats:sub>S donor as NaHS (50\u2009<jats:italic>\u03bc<\/jats:italic>M) for 4\u2009h. Cell migration after scratch was assessed. The expressions of <jats:italic>\u03b1<\/jats:italic>\u2010smooth muscle actin (<jats:italic>\u03b1<\/jats:italic>\u2010SMA), proliferating cell nuclear antigen (PCNA), collagen I, and collagen III were detected by immunofluorescence, real\u2010time PCR, and\/or Western blot. Intracellular superoxide anion and mitochondrial superoxide were evaluated by dihydroethidium (DHE) and MitoSOX staining, respectively. Mitochondrial membrane potential was detected by JC\u20101 staining. Apoptotic cells were detected by TDT\u2010mediated dUTP nick end labeling (TUNEL). The expressions of receptor interacting protein kinase 1 (RIPK1), RIPK3, and mixed lineage kinase domain\u2010like protein (MLKL) were measured by Western blot. We found that H<jats:sub>2<\/jats:sub>S production was impaired in both the plasma and skin of patients with keloid. In keloid fibroblasts and TGF\u2010<jats:italic>\u03b2<\/jats:italic><jats:sub>1<\/jats:sub>\u2010stimulated normal skin fibroblasts, exogenous H<jats:sub>2<\/jats:sub>S supplementation suppressed the expressions of <jats:italic>\u03b1<\/jats:italic>\u2010SMA, PCNA, collagen I, and collagen III, reduced intracellular superoxide anion and mitochondrial superoxide, improved the mitochondrial membrane potential, decreased the positive rate of TUNEL staining, and inhibited RIPK1 and RIPK3 expression as well as MLKL phosphorylation. Overall, H<jats:sub>2<\/jats:sub>S suppressed skin fibroblast proliferation <jats:italic>via<\/jats:italic> oxidative stress alleviation and necroptosis inhibition.<\/jats:p>","DOI":"10.1155\/2022\/7434733","type":"journal-article","created":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T22:35:15Z","timestamp":1655850915000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Hydrogen Sulfide Suppresses Skin Fibroblast Proliferation <i>via<\/i> Oxidative Stress Alleviation and Necroptosis 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