{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T10:11:43Z","timestamp":1769163103647,"version":"3.49.0"},"reference-count":58,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T00:00:00Z","timestamp":1668038400000},"content-version":"vor","delay-in-days":313,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Lam Kin Chung. Jet King-Shing Ho Glaucoma Treatment and Research Centre"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p>Primary open\u2010angle glaucoma (POAG) is the most common form of glaucoma, for which elevated intraocular pressure (IOP) is a major risk factor. IOP is mainly regulated by dynamic balance of aqueous humor (AH) production and outflow via the conventional trabecular meshwork\/Schlemm\u2019s canal (TM\/SC) pathway. Dysfunctions of TM cells due to endoplasmic reticulum (ER) stress have been demonstrated to increase the resistance of AH outflow, resulting in IOP elevation. Epigallocatechin\u20103\u2010gallate (EGCG), the most abundant polyphenolic component in green tea, has been shown to alleviate ER stress in several diseases while its potential roles in alleviating ER stress in TM cells have not been determined. In this study, we investigate the mitigation of tunicamycin\u2010induced ER stress in TM cells by EGCG. MTT assay was used to measure the cell viability of human TM (HTM) cells and primary porcine TM (PTM) cells. ER stress levels in both HTM cells and primary PTM cells were detected by quantitative real\u2010time PCR. The primary PTM cells isolated from porcine TM tissues were characterized by immunostaining. We found that 40\u2009<jats:italic>\u03bc<\/jats:italic>M and 80\u2009<jats:italic>\u03bc<\/jats:italic>M EGCG pretreatment substantially promoted HTM cell survival under 3\u2009<jats:italic>\u03bc<\/jats:italic>M tunicamycin\u2010induced ER stress. Pretreatment of 40\u2009<jats:italic>\u03bc<\/jats:italic>M EGCG markedly reduced the expression of ER stress markers <jats:italic>ATF4<\/jats:italic>, <jats:italic>HSPA5<\/jats:italic>, and <jats:italic>DDIT3,<\/jats:italic> evoked by 3\u2009<jats:italic>\u03bc<\/jats:italic>M tunicamycin in HTM cells. Furthermore, 40\u2009<jats:italic>\u03bc<\/jats:italic>M EGCG pretreatment significantly decreased the expressions of <jats:italic>ATF4<\/jats:italic>, <jats:italic>HSPA5<\/jats:italic>, and <jats:italic>DDIT3<\/jats:italic> at the mRNA level induced by 3\u2009<jats:italic>\u03bc<\/jats:italic>M tunicamycin and improved cell viability in primary PTM cells. Our results show that EGCG is capable of protecting TM cells from ER stress. EGCG provides a promising therapeutic option for POAG treatment.<\/jats:p>","DOI":"10.1155\/2022\/7435754","type":"journal-article","created":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T15:50:09Z","timestamp":1668095409000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Epigallocatechin\u20103\u2010Gallate Protects Trabecular Meshwork Cells from Endoplasmic Reticulum Stress"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1572-336X","authenticated-orcid":false,"given":"Linbin","family":"Zhou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4792-3668","authenticated-orcid":false,"given":"Jing Na","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1690-4963","authenticated-orcid":false,"given":"Lin","family":"Du","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7386-3868","authenticated-orcid":false,"given":"Bo Man","family":"Ho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6566-1019","authenticated-orcid":false,"given":"Danny Siu-Chun","family":"Ng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4298-8806","authenticated-orcid":false,"given":"Poemen P.","family":"Chan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4407-6907","authenticated-orcid":false,"given":"Clement C.","family":"Tham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7764-6382","authenticated-orcid":false,"given":"Chi Pui","family":"Pang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2903-3247","authenticated-orcid":false,"given":"Wai Kit","family":"Chu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,11,10]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2005.081224"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra0804630"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1350-9462(98)00014-7"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2014.05.013"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(17)31469-1"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c140014"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2019.10.002"},{"key":"e_1_2_9_8_2","first-page":"205","article-title":"Understanding trabecular meshwork physiology: a key to the control of intraocular pressure?","volume":"18","author":"Llobet A.","year":"2003","journal-title":"News in Physiological Sciences"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2014.07.014"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2016.05.012"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.15-16739"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.14-16220"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI58183"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.11-8837"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathol-012513-104649"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1209235"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2017.05.031"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.24923"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1250704"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1299.032"},{"key":"e_1_2_9_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3270"},{"key":"e_1_2_9_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2003.10.162"},{"key":"e_1_2_9_23_2","doi-asserted-by":"publisher","DOI":"10.18632\/aging.202677"},{"key":"e_1_2_9_24_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI69774"},{"key":"e_1_2_9_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2020.10.012"},{"key":"e_1_2_9_26_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2021.753955"},{"key":"e_1_2_9_27_2","doi-asserted-by":"publisher","DOI":"10.1080\/09553002.2018.1492755"},{"key":"e_1_2_9_28_2","doi-asserted-by":"publisher","DOI":"10.1177\/1535370215573394"},{"key":"e_1_2_9_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201700890"},{"key":"e_1_2_9_30_2","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2017.7388"},{"key":"e_1_2_9_31_2","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/6721530"},{"key":"e_1_2_9_32_2","first-page":"1043","article-title":"Human trabecular cells. I. Establishment in tissue culture and growth characteristics","volume":"18","author":"Polansky J. R.","year":"1979","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"e_1_2_9_33_2","first-page":"151","article-title":"In vitro localization of TIGR\/MYOC in trabecular meshwork extracellular matrix and binding to fibronectin","volume":"43","author":"Filla M. S.","year":"2002","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"e_1_2_9_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2018.03.001"},{"key":"e_1_2_9_35_2","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot095505"},{"key":"e_1_2_9_36_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_9_37_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-018-0935-8"},{"key":"e_1_2_9_38_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.24826"},{"key":"e_1_2_9_39_2","doi-asserted-by":"publisher","DOI":"10.1080\/13543776.2016.1203419"},{"key":"e_1_2_9_40_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26631"},{"key":"e_1_2_9_41_2","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/3480569"},{"key":"e_1_2_9_42_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M702390200"},{"key":"e_1_2_9_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2010.08.008"},{"key":"e_1_2_9_44_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046749"},{"key":"e_1_2_9_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2012.03.120"},{"key":"e_1_2_9_46_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgh168"},{"key":"e_1_2_9_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2017.453"},{"key":"e_1_2_9_48_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-12-280"},{"key":"e_1_2_9_49_2","doi-asserted-by":"publisher","DOI":"10.1207\/S15327914nc392_20"},{"key":"e_1_2_9_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2013.09.021"},{"key":"e_1_2_9_51_2","first-page":"1435","article-title":"(-)-Epigallocatechin gallate induced apoptosis in human adrenal cancer NCI-H295 cells through caspase-dependent and caspase-independent pathway","volume":"29","author":"Wu P. P.","year":"2009","journal-title":"Anticancer Research"},{"key":"e_1_2_9_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-19352-1"},{"key":"e_1_2_9_53_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep15270"},{"key":"e_1_2_9_54_2","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10071076"},{"key":"e_1_2_9_55_2","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2021.10669"},{"key":"e_1_2_9_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2015.06.002"},{"key":"e_1_2_9_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2017.03.005"},{"key":"e_1_2_9_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.4.1249-1259.2001"}],"container-title":["Oxidative Medicine and Cellular Longevity"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2022\/7435754.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2022\/7435754.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2022\/7435754","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T23:39:47Z","timestamp":1748907587000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2022\/7435754"}},"subtitle":[],"editor":[{"given":"Qiongming","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2022,1]]},"references-count":58,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["10.1155\/2022\/7435754"],"URL":"https:\/\/doi.org\/10.1155\/2022\/7435754","archive":["Portico"],"relation":{},"ISSN":["1942-0900","1942-0994"],"issn-type":[{"value":"1942-0900","type":"print"},{"value":"1942-0994","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1]]},"assertion":[{"value":"2022-06-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"7435754"}}