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Alopecia has become an exceedingly prevalent dermatological disorder. Etiologically, infection (bacterial and fungal infection), inflammation, and immune dysregulation are the main causes of immune-mediated hair loss. Treating hair loss has remained challenging as the available therapies are limited. Exosomes from adipose-derived stem cells (ADSC-Exos) have been used for treating neurodegenerative diseases and autoimmune diseases and in wound-healing treatments. However, the function and mechanism of ADSC-Exos in alopecia treatment remain unclear. This study is aimed at investigating the effects of ADSC-Exos on hair growth in vitro and in vivo for potentially treating immune-mediated alopecia and further exploring the underlying mechanism. Methods. Cell proliferation, migration, and apoptosis of dermal papilla cells (DPCs) that were treated with ADSC-Exos were detected using the cell counting kit-8 (CCK-8) assay, scratch wound-healing assay, and flow cytometry assay, respectively. A C57BL\/6 hair-depilated mouse model was established in vivo; then, ADSC-Exos were subcutaneously injected alone or in combined with minoxidil. The effects of ADSC-Exos on hair growth, pathological changes, and the related mechanism were investigated by HE staining, quantitative real\u2010time PCR (qRT-PCR), western blotting, and RNA sequencing (RNA-seq). Results. ADSC-Exos significantly promoted DPC proliferation and migration while also reducing apoptosis. In addition, compared with the control group, ADSC-Exos-treated mice had better hair growth, more hair follicles (HFs) and thicker dermis. RNA-seq revealed that the miR-22 and TNF-\u03b1 signaling pathways were markedly downregulated in DPCs after ADSC-Exos treatment. In addition, according to qRT-PCR and western blotting results, the Wnt\/\u03b2-catenin signaling pathway was activated in the skin of ADSC-Exos-treated mice. Conclusion. ADSC-Exos therapy positively affected the promotion of hair regrowth by regulating miR-22, the Wnt\/\u03b2-catenin signaling pathway, and the TNF-\u03b1 signaling pathway, implying that ADSC-Exos could be a promising cell-free therapeutic strategy for immune-mediated alopecia.<\/jats:p>","DOI":"10.1155\/2022\/7471246","type":"journal-article","created":{"date-parts":[[2022,2,3]],"date-time":"2022-02-03T07:50:18Z","timestamp":1643874618000},"page":"1-14","source":"Crossref","is-referenced-by-count":35,"title":["Exosomes Secreted from Adipose-Derived Stem Cells Are a Potential Treatment Agent for Immune-Mediated Alopecia"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1258-7676","authenticated-orcid":true,"given":"Yanqiao","family":"Li","sequence":"first","affiliation":[{"name":"Department of Dermatology, Tongji Hospital, School of Medicine, Tongji University, Shanghai 200092, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0302-7546","authenticated-orcid":true,"given":"Guangxing","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Medicine, Tongji University, Shanghai 200092, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3475-5300","authenticated-orcid":true,"given":"Qian","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Medicine, Tongji University, Shanghai 200092, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4471-0787","authenticated-orcid":true,"given":"Yun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute for Regenerative Medicine & Department of Joint Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200092, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4339-6629","authenticated-orcid":true,"given":"Lei","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery, Beijing Shijitan Hospital Affiliated to Capital Medical University, Beijing 100038, China"},{"name":"Department of Stem Cells and Regenerative Medicine, School of Medicine, Tongji University, Shanghai 200092, China"},{"name":"Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration, Ministry of Education of the People\u2019s Republic of China & Department of Orthopedics, Tongji Hospital, Tongji University School of Medicine, Shanghai 200092, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9155-2825","authenticated-orcid":true,"given":"Xin","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Tongji Hospital, School of Medicine, Tongji University, Shanghai 200092, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.13935"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines9030236"},{"issue":"4","key":"3","first-page":"356","article-title":"Diagnosing and treating hair loss","volume":"80","author":"A. 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