{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:34:37Z","timestamp":1790703277725,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T00:00:00Z","timestamp":1648512000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fundamental Research Funds for the Central Universities of Central South University","award":["2019zzts341"],"award-info":[{"award-number":["2019zzts341"]}]},{"name":"Fundamental Research Funds for the Central Universities of Central South University","award":["2021JJ31106"],"award-info":[{"award-number":["2021JJ31106"]}]},{"name":"Fundamental Research Funds for the Central Universities of Central South University","award":["2020LNJJ11"],"award-info":[{"award-number":["2020LNJJ11"]}]},{"name":"Fundamental Research Funds for the Central Universities of Central South University","award":["82073323"],"award-info":[{"award-number":["82073323"]}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2019zzts341"],"award-info":[{"award-number":["2019zzts341"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2021JJ31106"],"award-info":[{"award-number":["2021JJ31106"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2020LNJJ11"],"award-info":[{"award-number":["2020LNJJ11"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["82073323"],"award-info":[{"award-number":["82073323"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Clinical Research Foundation of Xiangya Hospital","award":["2019zzts341"],"award-info":[{"award-number":["2019zzts341"]}]},{"name":"Clinical Research Foundation of Xiangya Hospital","award":["2021JJ31106"],"award-info":[{"award-number":["2021JJ31106"]}]},{"name":"Clinical Research Foundation of Xiangya Hospital","award":["2020LNJJ11"],"award-info":[{"award-number":["2020LNJJ11"]}]},{"name":"Clinical Research Foundation of Xiangya Hospital","award":["82073323"],"award-info":[{"award-number":["82073323"]}]},{"name":"National Science and Nature Foundation","award":["2019zzts341"],"award-info":[{"award-number":["2019zzts341"]}]},{"name":"National Science and Nature Foundation","award":["2021JJ31106"],"award-info":[{"award-number":["2021JJ31106"]}]},{"name":"National Science and Nature Foundation","award":["2020LNJJ11"],"award-info":[{"award-number":["2020LNJJ11"]}]},{"name":"National Science and Nature Foundation","award":["82073323"],"award-info":[{"award-number":["82073323"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Oncology"],"published-print":{"date-parts":[[2022,3,29]]},"abstract":"<jats:p>Nucleotide excision repair (NER) is an important mediator for responsiveness of platinum-based chemotherapy. Our study is aimed at investigating the NER-related genes expression in ascites tumor cells and its application in the prediction of chemoresponse in high-grade serous ovarian cancer (HGSC) patients. The relationship between 16 NER-related genes and the prognosis of ovarian cancer was analyzed in the TCGA database. NER-related genes including HELQ and XAB2 expressions were determined via immunocytochemistry in ascites cell samples from 92 ovarian cancer patients prior to primary cytoreduction surgery. Kaplan-Meier analysis and Cox model were used to investigate the association between NER-related gene expression and prognosis\/chemotherapeutic response. Predicting models were constructed using a training cohort of 60 patients and validated in a validation cohort of 32 patients. We found that high expression of HELQ and XAB2 in the training cohort was associated with poor prognosis (for HELQ, <jats:inline-formula>\n                     <a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                        <a:mi>P<\/a:mi>\n                        <a:mo>=<\/a:mo>\n                        <a:mn>0.001<\/a:mn>\n                     <\/a:math>\n                  <\/jats:inline-formula>, <jats:inline-formula>\n                     <c:math xmlns:c=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\">\n                        <c:mtext>HR<\/c:mtext>\n                        <c:mo>=<\/c:mo>\n                        <c:mn>2.83<\/c:mn>\n                     <\/c:math>\n                  <\/jats:inline-formula>, 95% CI: 1.46-5.49; for XAB2, <jats:inline-formula>\n                     <e:math xmlns:e=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\">\n                        <e:mi>P<\/e:mi>\n                        <e:mo>=<\/e:mo>\n                        <e:mn>0.008<\/e:mn>\n                     <\/e:math>\n                  <\/jats:inline-formula>, <jats:inline-formula>\n                     <g:math xmlns:g=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\">\n                        <g:mtext>HR<\/g:mtext>\n                        <g:mo>=<\/g:mo>\n                        <g:mn>2.38<\/g:mn>\n                     <\/g:math>\n                  <\/jats:inline-formula>, 95% CI: 1.23-4.63) and platinum resistance (for HELQ, <jats:inline-formula>\n                     <i:math xmlns:i=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\">\n                        <i:mi>P<\/i:mi>\n                        <i:mo>&lt;<\/i:mo>\n                        <i:mn>0.001<\/i:mn>\n                     <\/i:math>\n                  <\/jats:inline-formula>; for XAB2, <jats:inline-formula>\n                     <k:math xmlns:k=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\">\n                        <k:mi>P<\/k:mi>\n                        <k:mo>=<\/k:mo>\n                        <k:mn>0.006<\/k:mn>\n                     <\/k:math>\n                  <\/jats:inline-formula>). In the validation cohort, the combination of HELQ and XAB2 (<jats:inline-formula>\n                     <m:math xmlns:m=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M7\">\n                        <m:mtext>AUC<\/m:mtext>\n                        <m:mo>=<\/m:mo>\n                        <m:mn>0.863<\/m:mn>\n                     <\/m:math>\n                  <\/jats:inline-formula>) showed the highest AUC. The expression levels of HELQ (RR 5.7, 95% CI 1.7-19.2) and XAB2 (RR 3.2, 95% CI 0.9-10.8) in ascites tumor cells were positively correlated to the risk of platinum resistance. In summary, we revealed that the expression levels of HELQ and XAB2 are candidate predictors for primary chemotherapy responsiveness and prognosis in HGSC. Ascites cytology is applicable as a promising method for chemosensitivity prediction in HGSC.<\/jats:p>","DOI":"10.1155\/2022\/7521934","type":"journal-article","created":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T00:20:10Z","timestamp":1648599610000},"page":"1-13","source":"Crossref","is-referenced-by-count":7,"title":["DNA Repair Protein HELQ and XAB2 as Chemoresponse and Prognosis Biomarkers in Ascites Tumor Cells of High-Grade Serous Ovarian Cancer"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3990-2346","authenticated-orcid":true,"given":"Fang","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Gynecology, Xiangya Hospital, Central South University, Changsha, Hunan, China"},{"name":"Gynecological Oncology Research and Engineering Center of Hunan Province, Changsha, Hunan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0566-9972","authenticated-orcid":true,"given":"Siyu","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Gynecology, Xiangya Hospital, Central South University, Changsha, Hunan, China"},{"name":"Gynecological Oncology Research and Engineering Center of Hunan Province, Changsha, Hunan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3962-6604","authenticated-orcid":true,"given":"Ming","family":"Lei","sequence":"additional","affiliation":[{"name":"Department of Gynecology, Xiangya Hospital, Central South University, Changsha, Hunan, China"},{"name":"Gynecological Oncology Research and Engineering Center of Hunan Province, Changsha, Hunan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3564-3893","authenticated-orcid":true,"given":"Qiongqiong","family":"He","sequence":"additional","affiliation":[{"name":"Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan, China"},{"name":"Department of Pathology, School of Basic Medicine, Central South University, Changsha, Hunan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2847-1982","authenticated-orcid":true,"given":"Lisha","family":"Wu","sequence":"additional","affiliation":[{"name":"Gynecological Oncology Research and Engineering Center of Hunan Province, Changsha, Hunan, China"},{"name":"Institute of Medical Science, Xiangya Hospital, Central South University, Changsha, Hunan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3474-422X","authenticated-orcid":true,"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Gynecology, Xiangya Hospital, Central South University, Changsha, Hunan, China"},{"name":"Gynecological Oncology Research and Engineering Center of Hunan Province, Changsha, Hunan, China"},{"name":"National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/S0889-8588(03)00058-3"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20040952"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24315"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2015.00240"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2014.00034"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63708-X"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1038\/nature08467"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-med-081313-121208"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1038\/35094000"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(00)00441-X"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1123"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1097\/GME.0000000000001595"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.7727\/wimj.2014.230"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-04-2236"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.4061\/2010\/201367"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1007\/s13318-019-00550-2"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1007\/s12072-016-9759-9"},{"key":"19","volume-title":"The NCCN Ovarian Cancer\/Fallopian Tube Cancer\/Primary Peritoneal Cancer clinical practice guidelines in oncology (Version 1.2021)[EB\/OL]","author":"K. 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