{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T17:30:04Z","timestamp":1769275804622,"version":"3.49.0"},"reference-count":45,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,3,15]],"date-time":"2022-03-15T00:00:00Z","timestamp":1647302400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870637"],"award-info":[{"award-number":["81870637"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Stem Cells International"],"published-print":{"date-parts":[[2022,3,15]]},"abstract":"<jats:p>Seeking for suitable conjunctival reconstruction substitutes to overcome the limitations of current substitutes, such as amniotic membrane, is urgent. Decellularized tissues have become a promising strategy for tissue engineering. In this study, we prepared decellularized porcine pericardium (DPP) scaffolds by the phospholipase A2 method and crosslinked them with aspartic acid (Asp) and human endothelial growth factor (hEGF) to enhance biological performance on the DPP, obtaining DPP-Asp-hEGF scaffolds. In vitro DPP showed lower apoptosis, highly desirable, well preservation of extracellular matrix components, and favorable macro-microstructure, which was confirmed by histology, immunofluorescence, electron microscopy, collagen and DNA quantification, and cytotoxicity assay, compared to the native porcine pericardium (NPP). The crosslinked efficacy of the DPP-Asp-hEGF was furtherer verified by in vitro experiments with Fourier transform infrared (FTIR) and X-ray diffraction (XRD). Through animal models of conjunctiva defect model, the DPP-Asp-hEGF revealed a closed, multilayer epithelium with an equal amount of goblet cells and no indication for conjunctival scarring after 28 days, compared to amniotic membrane (AM) groups and sham groups. These results suggested that DPP-Asp-hEGF can offer a good conjunctival reconstructive substitute both in structure and in function.<\/jats:p>","DOI":"10.1155\/2022\/7571146","type":"journal-article","created":{"date-parts":[[2022,3,16]],"date-time":"2022-03-16T04:35:14Z","timestamp":1647405314000},"page":"1-11","source":"Crossref","is-referenced-by-count":9,"title":["Crosslinked Decellularized Porcine Pericardium as a Substrate for Conjunctival Reconstruction"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6410-8123","authenticated-orcid":true,"given":"Fangyuan","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2347-7760","authenticated-orcid":true,"given":"Jingyue","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China"},{"name":"The Fifth Affiliated Hospital of Southern Medical University, Guangzhou 510900, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4265-6396","authenticated-orcid":true,"given":"Lishi","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China"},{"name":"Shenzhen Eye Hospital, Affiliated Hospital of Jinan University, Shenzhen 518040, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1101-6158","authenticated-orcid":true,"given":"Ying","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7474-9637","authenticated-orcid":true,"given":"Yuying","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7163-3436","authenticated-orcid":true,"given":"Yuanting","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2670-5514","authenticated-orcid":true,"given":"Rijia","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0184-4072","authenticated-orcid":true,"given":"Jian","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5234-6383","authenticated-orcid":true,"given":"Qing","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"issue":"5","key":"1","article-title":"The conjunctival extracellular matrix, related disorders and development of substrates for conjunctival restoration","volume":"6","author":"A. K. Makuloluwa","year":"2021","journal-title":"The Ocular Surface"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtos.2020.08.009"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.8b00015"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2018.0190"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0111846"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2019.07.018"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2019.08.027"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/j.jeurceramsoc.2017.11.001"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1002\/term.3103"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201902491"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtos.2020.07.020"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2019.11.006"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2017.11.054"},{"issue":"7","key":"14","first-page":"310","article-title":"Modified collagen gel-reinforced decellularized amniotic membrane improves limbal stem cell transplantation in a rabbit model of corneal epithelial defect","volume":"61","author":"D. Long","year":"2020","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1007\/s00417-015-3201-1"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2021.111938"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1007\/s10561-017-9675-9"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1007\/s10561-019-09792-5"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1093\/rb\/rbv005"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1177\/0885328220979273"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2018.03.052"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-016-5750-1"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1177\/2041731419827791"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1177\/2041731417724011"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2016.11.210"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1940.00860130586006"},{"issue":"2","key":"27","first-page":"363","article-title":"Histological, immunohistochemical and clinical considerations on amniotic membrane transplant for ocular surface reconstruction","volume":"58","author":"E. L. E. N. A. \u015eapte","year":"2017","journal-title":"Revue Roumaine De Morphologie Et Embryologie"},{"key":"28","article-title":"Artificial polymeric scaffolds as extracellular matrix substitutes for autologous","volume":"57","author":"M. He","year":"2016","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"29","article-title":"A composite tissue engineered bone material consisting of bone mesenchymal stem","volume":"26, article e9246661","author":"C. Zhou","year":"2020","journal-title":"Medical Science Monitor: International Medical Journal of Experimental and Clinical Research"},{"key":"30","doi-asserted-by":"crossref","DOI":"10.1080\/02713683.2020.1775258","article-title":"Eye socket regeneration and reconstruction","volume":"45","author":"M. Borrelli","year":"2020","journal-title":"Current Eye Research"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1093\/rb\/rbaa011"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0105964"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2017.5073"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.02.014"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.01.057"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1111\/xen.12564"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2009.03.003"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25184166"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.3390\/genes11090959"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1038\/eye.2000.141"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21124404"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1016\/S1084952102000940"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-2489-x"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.4238\/2015.October.29.2"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202100100"}],"container-title":["Stem Cells International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/sci\/2022\/7571146.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/sci\/2022\/7571146.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/sci\/2022\/7571146.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,16]],"date-time":"2022-03-16T04:35:22Z","timestamp":1647405322000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/sci\/2022\/7571146\/"}},"subtitle":[],"editor":[{"given":"Cinzia","family":"Marchese","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2022,3,15]]},"references-count":45,"alternative-id":["7571146","7571146"],"URL":"https:\/\/doi.org\/10.1155\/2022\/7571146","relation":{},"ISSN":["1687-9678","1687-966X"],"issn-type":[{"value":"1687-9678","type":"electronic"},{"value":"1687-966X","type":"print"}],"subject":[],"published":{"date-parts":[[2022,3,15]]}}}