{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:50:51Z","timestamp":1747191051580,"version":"3.40.5"},"reference-count":37,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,7,6]],"date-time":"2022-07-06T00:00:00Z","timestamp":1657065600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Computational Intelligence and Neuroscience"],"published-print":{"date-parts":[[2022,7,6]]},"abstract":"<jats:p>Middle cerebral artery occlusion (MCAO) induced brain ischemia-reperfusion model in Mice is essential for understanding the pathology of stroke and investigating potential treatments, in which a variety of methods may be employed to block the middle cerebral artery (MCA), the most common being through the insertion of a monofilament; however, in vivo ischemia-reperfusion models are associated, particularly in mice, with high variability in lesion volume and high mortality. We aimed to optimise a mouse model of cerebral ischemia-reperfusion, addressing issues of mouse survival, model reproducibility, and consistency. The model was optimised in two ways: first, insert the monofilament directly through the internal carotid artery rather than through the external or common carotid artery, and second, by extending the length of the silicone coating on the monofilament, the length of the silicone coating enables embolization of the beginning of the middle cerebral artery, as well as the anterior cerebral artery and part of the posterior communicating artery. Results: We assessed various parameters, including blood flow changes in the middle cerebral artery, stability of the infarct area, correlation between infarct volume percentages and neurological deficit scores, mortality, weight changes, and wellbeing. We found that optimisation of the surgical procedure may improve mouse wellbeing and reduce mortality, through reduced weight loss and decrease the variability. In conclusion, we suggest that the optimisation of the model is superior for the study of both short and long-term outcomes of ischemic stroke. These results have considerable implications on stroke model selection for researchers.<\/jats:p>","DOI":"10.1155\/2022\/7594969","type":"journal-article","created":{"date-parts":[[2022,7,6]],"date-time":"2022-07-06T22:50:07Z","timestamp":1657147807000},"page":"1-10","source":"Crossref","is-referenced-by-count":0,"title":["Optimisation of a Mouse Model of Cerebral Ischemia-Reperfusion to Address Issues of Survival and Model Reproducibility and Consistency"],"prefix":"10.1155","volume":"2022","author":[{"given":"Zhenqian","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Neurology, The Second Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mo","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Second Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xu","family":"Duan","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Second Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yujia","family":"Zhai","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Second Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Second Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zuowei","family":"Duan","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Second Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiang","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Second Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4439-0453","authenticated-orcid":true,"given":"Haiyan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Second Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcna.2018.10.001"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.0000441139.02102.80"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2202-14-41"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.14-0258"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-200006000-00006"},{"issue":"3","key":"6","first-page":"253","article-title":"[YAG laser-induced reperfusion of photothrombotic middle cerebral artery occlusion in rats]","volume":"53","author":"H. Yao","year":"2001","journal-title":"N\u014d to shinkei = Brain and nerve"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1007\/s12975-013-0315-9"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2014.10.001"},{"key":"9","first-page":"203","article-title":"A novel mouse model of thromboembolic stroke","volume-title":"J Neurosci Methods","author":"Y. Chen"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2012.09.006"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1161\/strokeaha.111.635250"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1212\/wnl.42.2.289"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.3791\/2879"},{"issue":"2","key":"14","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.jneumeth.2009.07.033","article-title":"Novel rat middle cerebral artery occlusion model\u2009:\u2009trans-femoral artery approach combined with preservation of the external carotid artery","volume":"184","author":"N. Shimamura","year":"2010","journal-title":"Journal of Neuroscience Methods"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.2147\/ndt.s118531"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0148503"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1227\/00006123-199603000-00021"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.029108"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.3995\/jstroke.8.1"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1161\/01.str.20.1.84"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2003.10.028"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2015.04.008"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1161\/01.str.0000083625.54851.9a"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2005.05.013"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1007\/s12975-011-0102-4"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1177\/0271678x16660986"},{"issue":"5","key":"27","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1097\/00004647-199805000-00012","article-title":"Cerebral ischemia after bilateral carotid artery occlusion and intraluminal suture occlusion in mice: evaluation of the patency of the posterior communicating artery","volume":"18","author":"K. Kaawa","year":"1998","journal-title":"Journal of Cerebral Blood Flow and Metabolism"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.3171\/2011.11.jns111167"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.3791\/2879"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1161\/hs1101.098367"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1994.88"},{"issue":"6","key":"32","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1080\/01616412.1997.11740874","article-title":"Monofilament intraluminal middle cerebral artery occlusion in the mouse","volume":"19","author":"W. M. Clark","year":"1997","journal-title":"Neurological Research"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2990.2000.00221.x"},{"issue":"3","key":"34","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1007\/s12975-012-0234-1","article-title":"Distal occlusion of the middle cerebral artery in mice: are we ready to assess long-term functional outcome","volume":"4","author":"A. Rosell","year":"2013","journal-title":"Transl Stroke Res"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1161\/strokeaha.119.026652"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2008.06.021"},{"issue":"5","key":"37","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1038\/jcbfm.2010.10","article-title":"Faber JEWide genetic variation in the native pial collateral circulation is a major determinant of variation in severity of stroke. J Cereb Blood Flow Metab 30:923-934","volume":"30","author":"Z. Hua","year":"2010","journal-title":"Journal of the International Society of Cerebral Blood Flow & Metabolism"}],"container-title":["Computational Intelligence and Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/cin\/2022\/7594969.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/cin\/2022\/7594969.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/cin\/2022\/7594969.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,6]],"date-time":"2022-07-06T22:50:15Z","timestamp":1657147815000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/cin\/2022\/7594969\/"}},"subtitle":[],"editor":[{"given":"Dong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2022,7,6]]},"references-count":37,"alternative-id":["7594969","7594969"],"URL":"https:\/\/doi.org\/10.1155\/2022\/7594969","relation":{},"ISSN":["1687-5273","1687-5265"],"issn-type":[{"type":"electronic","value":"1687-5273"},{"type":"print","value":"1687-5265"}],"subject":[],"published":{"date-parts":[[2022,7,6]]}}}