{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T21:53:09Z","timestamp":1788385989976,"version":"build-2803163510"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,10,14]],"date-time":"2022-10-14T00:00:00Z","timestamp":1665705600000},"content-version":"vor","delay-in-days":286,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"General Program Foundation from the Hangzhou City Health Bureau","award":["20210250"],"award-info":[{"award-number":["20210250"]}]},{"name":"General Program Foundation from the Hangzhou City Health Bureau","award":["20171226Y17"],"award-info":[{"award-number":["20171226Y17"]}]},{"name":"General Program Foundation from the Hangzhou City Health Bureau","award":["LGF20H030002"],"award-info":[{"award-number":["LGF20H030002"]}]},{"name":"General Program Foundation from the Science and Technology Bureau of Hangzhou City","award":["20210250"],"award-info":[{"award-number":["20210250"]}]},{"name":"General Program Foundation from the Science and Technology Bureau of Hangzhou City","award":["20171226Y17"],"award-info":[{"award-number":["20171226Y17"]}]},{"name":"General Program Foundation from the Science and Technology Bureau of Hangzhou City","award":["LGF20H030002"],"award-info":[{"award-number":["LGF20H030002"]}]},{"name":"Public Welfare Program Foundation from the Science and Technology Bureau of Zhejiang Province","award":["20210250"],"award-info":[{"award-number":["20210250"]}]},{"name":"Public Welfare Program Foundation from the Science and Technology Bureau of Zhejiang Province","award":["20171226Y17"],"award-info":[{"award-number":["20171226Y17"]}]},{"name":"Public Welfare Program Foundation from the Science and Technology Bureau of Zhejiang Province","award":["LGF20H030002"],"award-info":[{"award-number":["LGF20H030002"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p>\n                    Recent evidence suggested that autologous concentrated growth factor (CGF), a new bioactive compound from autologous blood is used widely as an ingenious biomaterial in tissue regeneration with anti\u2010inflammatory properties. This study investigated whether CGF could be involved in the treatment of fistula healing in the anal fistula. For this purpose, the porcine anal fistula model was conducted using the rubber band ligation method and collected pig autogenic CGF to treat the fistulas. CGF treatment promoted fistula healing, which was reflected in the downregulation of inflammatory factors, upregulation of growth factors, and promoted epithelial\u2010mesenchymal transition with increased collagen synthesis. Besides, 16S rRNA gene sequencing analysis of fistula tissues between the control and CGF groups showed that the microbial populations exhibiting significant differences were\n                    <jats:italic>VadinCA02<\/jats:italic>\n                    ,\n                    <jats:italic>Blastomonas<\/jats:italic>\n                    ,\n                    <jats:italic>Deinococcus<\/jats:italic>\n                    ,\n                    <jats:italic>Devosia<\/jats:italic>\n                    ,\n                    <jats:italic>Sphingomonas<\/jats:italic>\n                    ,\n                    <jats:italic>Rubrobacteria<\/jats:italic>\n                    , and\n                    <jats:italic>GW_34<\/jats:italic>\n                    . CGF of volunteers were collected to process small interfering RNA\u2010 (siRNA\u2010) ERK or siRNA\u2010negative control transfected human skin fibroblasts (HSF). The results showed that CGF also promoted the proliferation and extracellular matrix\u2010related functions in HSF, as well as activated the MEK\/ERK pathway\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Finally, knockdown ERK reversed the effects of CGF in promoting wound healing in HSF. Collectively, our results suggest that the CGF as the bioactive compound from autologous blood exhibited great potential for repairing fistulas as well as promoting the proliferation and migration of human skin fibroblasts by triggering MEK\/ERK signaling. These findings provided a fresh perspective for understanding the role of CGF in the management of fistulas.\n                  <\/jats:p>","DOI":"10.1155\/2022\/7660118","type":"journal-article","created":{"date-parts":[[2022,10,14]],"date-time":"2022-10-14T18:50:24Z","timestamp":1665773424000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Autologous Bioactive Compound Concentrated Growth Factor Ameliorates Fistula Healing of Anal Fistula in a Pig Model and Promotes Proliferation and Migration of Human Skin Fibroblasts via Regulating the MEK\/ERK Pathway"],"prefix":"10.1155","volume":"2022","author":[{"given":"Xiufeng","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianming","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Houdong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenfeng","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuxian","family":"Shao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2884-7743","authenticated-orcid":false,"given":"Zhong","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,10,14]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1080\/17474124.2018.1500175"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijsu.2019.08.003"},{"key":"e_1_2_9_3_2","volume-title":"Anal Fistula and Abscess. 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