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Herein, we report the mechanism of action of the traditional Chinese medicine extract Forsythiaside A in the treatment of male infertility and experimental verification. We first obtained 95 intersection genes between the target protein of Forsythiaside A and the target genes of male infertility and screened 13 key genes. In molecular docking, Forsythiaside A can each have a higher total docking score with 12 key genes and have a better combination. These 95 intersection genes are mainly related to biological processes such as response to peptide hormone, response to oxidative stress, and participation in the oxidative stress of the forkhead box O (FoxO) signaling pathway. Therefore, we use ornidazole to induce an experimental model of oligoasthenospermia in rats and use different concentrations of Forsythiaside A to intervene. We proved that the semen quality and superoxide dismutase (SOD) activities of model group rats were significantly lower than those of the blank group, and semen quality and SOD activities of the low-dose group and high-dose group were significantly higher than those of the model group. The malondialdehyde (MDA) level of model group rats was significantly higher than that of blank group, while the MDA levels of the low-dose group and high-dose group were significantly lower than that of the model group. Forsythoside A is a potential drug substance for male infertility and improves the semen quality, MDA levels, and SOD activities of rats with oligoasthenospermia.<\/jats:p>","DOI":"10.1155\/2022\/7723358","type":"journal-article","created":{"date-parts":[[2022,10,6]],"date-time":"2022-10-06T19:35:29Z","timestamp":1665084929000},"page":"1-14","source":"Crossref","is-referenced-by-count":2,"title":["The Mechanism and Experimental Validation of Forsythoside A in the Treatment of Male Infertility Were Analyzed Based on Network Pharmacology and Molecular Docking"],"prefix":"10.1155","volume":"2022","author":[{"given":"Zhen","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Medical Ultrasound, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China"},{"name":"Department of Medical Ultrasound, Guangxi International Zhuang Medical Hospital, Nanning, Guangxi Zhuang Autonomous Region, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueling","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Medical Ultrasound, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, Guangxi Zhuang Autonomous Region, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiwang","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Andrology, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, Guangxi Zhuang Autonomous Region, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haoming","family":"Li","sequence":"additional","affiliation":[{"name":"Guangxi University of Chinese Medicine, Nanning, Guangxi Zhuang Autonomous Region, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruizhi","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Medical Ultrasound, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rong","family":"Wen","sequence":"additional","affiliation":[{"name":"Department of Medical Ultrasound, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiping","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Medical Ultrasound, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haihui","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Medical Ultrasound, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun","family":"He","sequence":"additional","affiliation":[{"name":"Department of Medical Ultrasound, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9873-1631","authenticated-orcid":true,"given":"Hong","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Medical Ultrasound, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1007\/s11745-014-3922-7"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-0659-8_2"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmaa022"},{"issue":"4","key":"4","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41374-019-0353-3","article-title":"Chondrial citrate synthase initiates calcium oscillation and suppresses age-dependent sperm dysfunction","volume":"100","year":"2020","journal-title":"Laboratory Investigation"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1126\/science.1108190"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.2741\/e223"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1007\/s40620-016-0286-5"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1038\/nrurol.2017.98"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2013.04.003"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2019.11.008"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1055\/s-0033-1345277"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21207796"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.3390\/jcm7090239"},{"issue":"10","key":"14","first-page":"56","article-title":"Effects of forsythiaside on the expression of IFN-\u03b1and Mx1","volume":"2012","author":"Y. 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