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Colorectal cancer (CRC), a common digestive tract tumor that contains colon and rectal cancer, is one of the three most common cancers globally. circRNAs are involved in the occurrence and development of CRC, but the mechanism of how they participate in this process remains unclear. Methods. We adopted PCR for expression measure, CCK-8 for cell proliferation detection, Transwell for cell migration and invasion detection, and dual-luciferase reporter assays to detect the potential downstream targets of CCDC66 in CRC. Results. This study showed that circRNA CCDC66 was overexpressed in CRC tissues, and after knockdown, it inhibited the proliferation, migration, and invasion of CRC cells (RKO and HCT-116) in vitro. In addition, the dual-luciferase reporter assay showed that there was a binding site between circCCDC66 and miR-370, as well as between miR-370 and murine double minute 4 (MDM4). That is, circCCDC66 upregulated the expression of MDM4 through competitively binding to miR-370. The expression of circCCDC66 in CRC tissues was positively correlated with MDM4 and negatively correlated with miR-370. Conclusion. In summary, our results indicate that circCCDC66 is a key upregulation of CRC. circCCDC66 upregulates MDM4 through competitive binding to miR-370, thereby enhancing the metastatic ability of CRC cells and promoting the development of CRC.<\/jats:p>","DOI":"10.1155\/2022\/7723995","type":"journal-article","created":{"date-parts":[[2022,1,12]],"date-time":"2022-01-12T01:05:06Z","timestamp":1641949506000},"page":"1-9","source":"Crossref","is-referenced-by-count":11,"title":["Circular RNA CCDC66 Improves Murine Double Minute 4 (MDM4) Expression through Targeting miR-370 in Colorectal Cancer"],"prefix":"10.1155","volume":"2022","author":[{"given":"Yang","family":"Mo","sequence":"first","affiliation":[{"name":"Teaching and Research Section of Clinical Nursing, Xiangya Hospital of Central South University, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qin","family":"Lu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chemo\/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, Hunan University, Changsha 410082, China"},{"name":"GeneTalks Biotech Co., Ltd., Changsha, Hunan 410000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Blood Transfusion Department, Zibo Central Hospital, Zibo, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Essential Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youming","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Essential Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Essential Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ran","family":"Tao","sequence":"additional","affiliation":[{"name":"Department of Essential Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9791-4663","authenticated-orcid":true,"given":"Weidong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Essential Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9690-4682","authenticated-orcid":true,"given":"Yiming","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chemo\/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, Hunan University, Changsha 410082, China"},{"name":"GeneTalks Biotech Co., Ltd., Changsha, Hunan 410000, China"},{"name":"Xiangnan University, Chenzhou, Hunan 410008, China"},{"name":"GeneMind Biosciences Company Limited, ShenZhen 518000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2017.361"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1080\/10409238.2016.1276882"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1111\/1759-7714.13251"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.01.055"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2020.1789819"},{"issue":"7","key":"6","first-page":"3945","article-title":"Roles of circular RNA in breast cancer: present and future","volume":"11","author":"Z. 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