{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T14:03:27Z","timestamp":1787580207092,"version":"build-2736575974"},"reference-count":45,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,12,14]],"date-time":"2022-12-14T00:00:00Z","timestamp":1670976000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Parasitology Research"],"published-print":{"date-parts":[[2022,12,14]]},"abstract":"<jats:p>Background. The genetic variation of Plasmodium falciparum has been studied to assess local malaria transmission genetic profile using evidence-based intervention measures. However, there are no known previous reports of P. falciparum polymorphism in Badewacho and Boset districts, Southern Ethiopia. The purpose of this study was to determine the genetic diversity of the merozoite surface protein-1 and -2 (msp-1 and msp-2) allelic families in P. falciparum isolates from an asymptomatic populations. Methods. This study was conducted from finger-prick blood samples spotted on 3\u2009mm Whatman filter paper collected during a community-based cross-sectional study. Nested polymerase chain reaction amplification was used to type the allelic variants of msp-1 and msp-2. Results. From 669 asymptomatic study participants, a total of 50 samples positive for P. falciparum were included for molecular analysis. Of 50 positive samples, 43 P. falciparum isolates were successfully amplified for the msp-1 and msp-2 allelic families. A total of twelve different allele sizes (75\u2013250\u2009bp) were identified within the three allelic families of msp-1, whereas ten different allele sizes (250\u2013500\u2009bp) were detected within the two allelic families of msp-2. MAD20 had a higher allelic proportion, 65% among allelic families of msp-1, whereas the 3D7 allelic family 90.7% was higher in msp-2. A slightly higher frequency of polyclonal infection 53.5% was found in msp-2 allelic family, whereas a low proportion polyclonal infection 46.5% was found in msp-1 allelic family. The overall mean multiplicity of infection (MOI) for msp-1 and msp-2 was identical (MOI\u2009=\u20091.56). Correspondingly, the expected heterozygosity (He) value for msp-1 (He\u2009=\u20090.23) and msp-2 (He\u2009=\u20090.22) was almost similar. Conclusions. The findings of this study revealed low genetic diversity of the msp-1 and msp-2 allelic families in P. falciparum isolates. However, continued monitoring status of the local genetic diversity profile in the P. falciparum population is required to support current malaria control and elimination strategies.<\/jats:p>","DOI":"10.1155\/2022\/7728975","type":"journal-article","created":{"date-parts":[[2022,12,14]],"date-time":"2022-12-14T18:20:08Z","timestamp":1671042008000},"page":"1-8","source":"Crossref","is-referenced-by-count":7,"title":["Genetic Diversity of Merozoite Surface Protein-1 and -2 Genes in Plasmodium falciparum Isolates among Asymptomatic Population in Boset and Badewacho Districts, Southern Ethiopia"],"prefix":"10.1155","volume":"2022","author":[{"given":"Tsegaye","family":"Chekol","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory Sciences, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1460-5716","authenticated-orcid":true,"given":"Gezahegn Solomon","family":"Alemayehu","sequence":"additional","affiliation":[{"name":"Research and Community Service Center, College of Health Science Defense University, Bishoftu, Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weynshet","family":"Tafesse","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science, College of Medicine and Health Sciences, Wachamo University, Hossna, Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gudeta","family":"Legesse","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science, College of Medicine and Health Sciences, Arsi University, Assela, Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2406-5971","authenticated-orcid":true,"given":"Biruk","family":"Zerfu","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2126-5617","authenticated-orcid":true,"given":"Temesgen","family":"File","sequence":"additional","affiliation":[{"name":"Department of Applied Biology, Adama Science and Technology University, Adama, Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mistire","family":"Wolde","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1216-8711","authenticated-orcid":true,"given":"Lemu","family":"Golassa","sequence":"additional","affiliation":[{"name":"Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","volume-title":"World Malaria Report 2021","author":"WHO","year":"2021"},{"key":"2","volume-title":"Ethiopia National Malaria Indicator Survey 2015","author":"Federal Ministry of Health","year":"2016"},{"issue":"1","key":"3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12936-019-2844-5","article-title":"Genetic diversity and drug resistance surveillance of Plasmodium falciparum for malaria elimination: is there an ideal tool for resource-limited sub-Saharan Africa?","volume":"18","author":"T. 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