{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:50:58Z","timestamp":1747191058669,"version":"3.40.5"},"reference-count":27,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,2,28]],"date-time":"2022-02-28T00:00:00Z","timestamp":1646006400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Healthcare Engineering"],"published-print":{"date-parts":[[2022,2,28]]},"abstract":"<jats:p>Ectopic pregnancy (EP) is associated with significant morbidity and mortality, but the molecular mechanism of this condition is still unclear. miR-196b, a hot research direction for the past few years, participates in the occurrence of various diseases but whether it plays a regulatory role in EP is still unclear. This research was set to investigate the expression and potential value of miR-196b in EP. qRT-PCR was utilized to determine the relative expression of miR-196b in peripheral blood of EP patients and to observe the expression changes of miR-196b before and after treatment. Correlation analysis of miR-196b with HCG and progesterone was performed. Logistic regression analysis was applied to independent risk factors affecting EP patients. TargetScan was utilized to predict the downstream target genes of miR-196b, and GO and KEGG analysis was carried out using the R language pack. qRT-PCR showed that miR-196b expression in peripheral blood of EP patients was lower than that of normal people. miR-196b expression in patients after treatment was notably higher than that before treatment. In addition, correlation analysis showed that miR-196b was positively correlated with the expression of HCG, progesterone, and estradiol. Risk factor analysis revealed that abortion history, pelvic inflammatory disease history, lower abdominal surgery history, and miR-196b were independent risk factors for EP, and the AUC of the combined ROC curve was 0.899. GO function enrichment and KEGG signal pathway enrichment found 10 potential functions and 2 potential signal pathways of miR-196b. miR-196b is expressed in EP patients, is differentially expressed according to the change in EP condition, and is expected to become a promising index for clinical diagnosis of EP.<\/jats:p>","DOI":"10.1155\/2022\/7797484","type":"journal-article","created":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T03:50:06Z","timestamp":1646106606000},"page":"1-8","source":"Crossref","is-referenced-by-count":3,"title":["Effect and Role of miR-196b in Ectopic Pregnancy"],"prefix":"10.1155","volume":"2022","author":[{"given":"Shan","family":"Deng","sequence":"first","affiliation":[{"name":"Department of Gynaecology and Obstetrics, The Second Affiliated Hospital of Guangdong Medical University, Zhanjiang 524000, Guangdong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaofeng","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Gynaecology and Obstetrics, The Second Affiliated Hospital of Guangdong Medical University, Zhanjiang 524000, Guangdong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoling","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Gynaecology and Obstetrics, The Second Affiliated Hospital of Guangdong Medical University, Zhanjiang 524000, Guangdong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaomiao","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Gynaecology and Obstetrics, The Second Affiliated Hospital of Guangdong Medical University, Zhanjiang 524000, Guangdong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengru","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Gynaecology and Obstetrics, The Second Affiliated Hospital of Guangdong Medical University, Zhanjiang 524000, Guangdong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoman","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Gynaecology and Obstetrics, The Second Affiliated Hospital of Guangdong Medical University, Zhanjiang 524000, Guangdong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3471-8139","authenticated-orcid":true,"given":"Mushi","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Urological Surgery, The Affiliated Hospital of Guangdong Medical University, Zhanjiang 524000, Guangdong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ogc.2019.02.002"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1097\/grf.0b013e3182516d7b"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1556\/oh.2014.29933"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2015.01.041"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2011.179283"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2017.08.034"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1139\/bcb-2018-0354"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/6121696"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2018.5998"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20171312"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2018.8671"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0102185"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0000000000002464"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1097\/gco.0b013e3283481212"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmp057"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1088\/1478-3975\/aa6f21"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.2174\/1570159X15666170703102410"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-15-3367"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.05.111"},{"issue":"1","key":"21","first-page":"42","article-title":"Influence of hCG glycosylation on its functions in female reproduction","volume":"82","author":"I. 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