{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T06:19:15Z","timestamp":1767853155479,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,3,26]],"date-time":"2022-03-26T00:00:00Z","timestamp":1648252800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81774141"],"award-info":[{"award-number":["81774141"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81904046"],"award-info":[{"award-number":["81904046"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2022,3,26]]},"abstract":"<jats:p>Background. Tetramethylpyrazine (TMP) is an alkaloid in Chinese herbal medicine, which possesses antiplatelet activity. TMP inhibits platelet activation in many ways. The platelet P2Y12 receptor for adenosine 5\u2032 diphosphate (ADP) plays a central role in platelet function, hemostasis, and thrombosis. Here, we investigated the inhibitory effect of TMP on P2Y12 receptor-related platelet function. Methods. The inhibitory potential of TMP was assessed using agonist-induced platelet aggregation, flow cytometric analysis of CD62p expression, PAC-1 activation, and fibrin clot retraction. After the P2Y12 receptor-related signaling pathway was inhibited using the blocker, platelet activation was studied by platelet aggregation, CD62p expression, and PAC-1 activation. The secretion of cyclic adenosine monophosphate (cAMP) was measured using enzyme-linked immunosorbent assay (ELISA), and the expression of signaling pathway protein, phosphorylation of vasodilator-stimulated phosphoprotein, and phosphorylation of Akt were investigated using western blotting. The release of platelet inflammatory mediators was measured using ELISA. Results. TMP had an antiplatelet effect by inhibiting ADP-induced aggregation, P-selectin secretion, and glycoprotein (GP) IIb\/IIIa expression and reducing the release of atherosclerotic-related inflammatory mediators (sCD40L and IL-1\u03b2). TMP decreased the area of clot retraction, reflecting inhibition of GPIIb\/IIIa activation. TMP inhibited adenosine diphosphate-induced platelet activation via increased cAMP production, VASPser157 phosphorylation, and Akt dephosphorylation. Conclusion. TMP selectively inhibits ADP-induced platelet activation via P2Y12 receptor-related signaling pathways.<\/jats:p>","DOI":"10.1155\/2022\/7941039","type":"journal-article","created":{"date-parts":[[2022,3,26]],"date-time":"2022-03-26T18:20:07Z","timestamp":1648318807000},"page":"1-11","source":"Crossref","is-referenced-by-count":6,"title":["Antiplatelet Activity of Tetramethylpyrazine via Regulation of the P2Y12 Receptor Downstream Signaling Pathway"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6582-990X","authenticated-orcid":true,"given":"Baoyi","family":"Guan","sequence":"first","affiliation":[{"name":"China Heart Institute of Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing 100091, China"},{"name":"Cardiovascular Diseases Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China"},{"name":"National Clinical Research Center for Chinese Medicine Cardiology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Gao","sequence":"additional","affiliation":[{"name":"China Heart Institute of Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing 100091, China"},{"name":"Cardiovascular Diseases Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China"},{"name":"National Clinical Research Center for Chinese Medicine Cardiology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Tan","sequence":"additional","affiliation":[{"name":"China Heart Institute of Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing 100091, China"},{"name":"Cardiovascular Diseases Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China"},{"name":"National Clinical Research Center for Chinese 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