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The pathogenesis of slow transit constipation (STC) is associated with exosomal miR-34c-5p. Electroacupuncture (EA) improves gastrointestinal motility in gastrointestinal disorders, especially STC. Our study aimed to explore the mechanism by which EA improves intestinal motility by modulating the release of exosomes and the transmission of exosomal miR-34c-5p. Methods. Fifty rats were randomly divided into five groups. STC model rats were induced, and GW4869, the exosome release inhibitor, was used to inhibit the release of exosome. The serum exosomes were authenticated under a transmission electron microscope and nanoparticle tracking analysis. RT-qPCR detected the expression of miR-34c-5p in serum exosomes and colonic tissues. The fecal number in 24 hours, Bristol scores, and intestinal transit rates were used to assess intestinal motility. Subsequently, hematoxylin and eosin (H&amp;E) staining was used to examine the colonic mucosal histology. Finally, the expression of stem cell factor (SCF) and receptor tyrosine kinase (c-Kit) protein was measured using immunohistochemistry staining. Results. We found that EA upregulated exosomal miR-34c-5p in serum and downregulated miR-34c-5p in colonic tissues (<jats:inline-formula>\n                     <a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                        <a:mi>P<\/a:mi>\n                        <a:mo>&lt;<\/a:mo>\n                        <a:mn>0.01<\/a:mn>\n                     <\/a:math>\n                  <\/jats:inline-formula>). EA improved fecal numbers in 24 hours, Bristol scores, and intestinal transit rates in STC rats (<jats:inline-formula>\n                     <c:math xmlns:c=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\">\n                        <c:mi>P<\/c:mi>\n                        <c:mo>&lt;<\/c:mo>\n                        <c:mn>0.01<\/c:mn>\n                     <\/c:math>\n                  <\/jats:inline-formula>). EA recovered the colonic histological structure and enhanced the expression of SCF and c-Kit protein (<jats:inline-formula>\n                     <e:math xmlns:e=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\">\n                        <e:mi>P<\/e:mi>\n                        <e:mo>&lt;<\/e:mo>\n                        <e:mn>0.01<\/e:mn>\n                     <\/e:math>\n                  <\/jats:inline-formula>). The therapeutic effect of EA was attenuated after inhibiting the release of the exosome. Conclusion. Our results indicated that EA improves intestinal motility in STC rats by transporting of exosomal miR-34c-5p targeting the SCF\/c-Kit signaling pathway.<\/jats:p>","DOI":"10.1155\/2022\/8043841","type":"journal-article","created":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T19:50:08Z","timestamp":1663012208000},"page":"1-10","source":"Crossref","is-referenced-by-count":8,"title":["Electroacupuncture Improves Intestinal Motility through Exosomal miR-34c-5p Targeting SCF\/c-Kit Signaling Pathway in Slow Transit Constipation Model Rats"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7877-6325","authenticated-orcid":true,"given":"Hongjun","family":"Kuang","sequence":"first","affiliation":[{"name":"The First Hospital of Hunan University of Chinese Medicine, Changsha 410007, Hunan Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengshun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Acupuncture and Tuina School-Third Teaching Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Sichuan Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"The First Hospital of Hunan University of Chinese Medicine, Changsha 410007, Hunan Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huzhi","family":"Cai","sequence":"additional","affiliation":[{"name":"The First Hospital of Hunan University of Chinese Medicine, Changsha 410007, Hunan Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Layuan","family":"Yang","sequence":"additional","affiliation":[{"name":"The First Hospital of Hunan University of Chinese Medicine, Changsha 410007, Hunan Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Yuan","sequence":"additional","affiliation":[{"name":"The First Hospital of Hunan University of Chinese Medicine, Changsha 410007, Hunan Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangyang","family":"Yuan","sequence":"additional","affiliation":[{"name":"The First Hospital of Hunan University of Chinese Medicine, Changsha 410007, Hunan Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutao","family":"Yang","sequence":"additional","affiliation":[{"name":"The First Hospital of Hunan University of Chinese Medicine, Changsha 410007, Hunan Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanyi","family":"Zuo","sequence":"additional","affiliation":[{"name":"Department of Acupuncture, Chongqing Traditional Chinese Medicine Hospital, Chongqing 400021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6490-3116","authenticated-orcid":true,"given":"Feng","family":"Zhong","sequence":"additional","affiliation":[{"name":"The First Hospital of Hunan University of Chinese Medicine, Changsha 410007, Hunan Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2012.10.028"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0036-1593436"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1007\/s40265-020-01305-z"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/j.cger.2013.10.001"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2007.10.020"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.3390\/jcm10081738"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1097\/mcg.0000000000001049"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21124540"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2021.12331"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.23.090506.123406"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-060308-103103"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21217893"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.7150\/thno.41580"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/7530102"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/7543049"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2022.115075"},{"issue":"2","key":"17","first-page":"141","article-title":"Effect of electroacupuncture on gastrointestinal motility in rats with slow transit constipation based on GDNF methylation modification","volume":"47","author":"Y. 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