{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:34:47Z","timestamp":1790793287265,"version":"4.1.0"},"reference-count":64,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,6,16]],"date-time":"2022-06-16T00:00:00Z","timestamp":1655337600000},"content-version":"vor","delay-in-days":166,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81572117"],"award-info":[{"award-number":["81572117"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871837"],"award-info":[{"award-number":["81871837"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p>\n                    Adipose mesenchymal stromal cell\u2010derived exosomes (ADSC\u2010Exos) have shown great potential in the treatment of oxidative stress induced by ischemia\u2010reperfusion injury. However, alleviation of testicular torsion injury by ADSC\u2010Exos has not been reported. Therefore, we investigated the protective effect of ADSC\u2010Exos against testicular torsion\u2010detorsion injury. ADSC\u2010Exos were isolated by ultracentrifugation and injected into torsion\u2010detorsion\u2010affected testes of rats. H&amp;E staining and sperm quality were used to evaluate the therapeutic effects of ADSC\u2010Exos, and tissue oxidative stress was measured by determining MDA and SOD levels. In addition, TUNEL staining and immunohistological analysis (Ki67, Cleaved Caspase\u20103, IL\u20106, IL\u201010, CCR7, and CD163) were used to clarify the effects of ADSC\u2010Exos on spermatogenic cell proliferation, apoptosis, and the inflammatory microenvironment\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Possible signaling pathways were predicted using sequencing technology and bioinformatics analysis. The predicted signaling pathways were validated\n                    <jats:italic>in vitro<\/jats:italic>\n                    by assessing the proliferation (EdU assay), migration (transwell assay and scratch test), and apoptosis (flow cytometry, TUNEL staining, and western blotting) of spermatogenic cells. The results showed that ADSC\u2010Exos alleviated testicular torsion\u2010detorsion injury by attenuating oxidative stress and the inflammatory response. In addition, ADSC\u2010Exos promoted the proliferation and migration of spermatogenic cells and inhibited their apoptosis by activating the PI3K\/AKT and MAPK\/ERK1\/2 signaling pathways.\n                  <\/jats:p>","DOI":"10.1155\/2022\/8065771","type":"journal-article","created":{"date-parts":[[2022,6,16]],"date-time":"2022-06-16T19:50:53Z","timestamp":1655409053000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Adipose Mesenchymal Stromal Cell\u2010Derived Exosomes Prevent Testicular Torsion Injury\n                    <i>via<\/i>\n                    Activating PI3K\/AKT and MAPK\/ERK1\/2 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