{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T15:55:50Z","timestamp":1768838150435,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,3,20]],"date-time":"2022-03-20T00:00:00Z","timestamp":1647734400000},"content-version":"vor","delay-in-days":78,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32172801"],"award-info":[{"award-number":["32172801"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31873000"],"award-info":[{"award-number":["31873000"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005089","name":"Beijing Municipal Natural Science Foundation","doi-asserted-by":"publisher","award":["6222019"],"award-info":[{"award-number":["6222019"]}],"id":[{"id":"10.13039\/501100005089","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. Our previous studies demonstrated that melatonin could effectively ameliorate sleep deprivation\u2010 (SD\u2010) caused oxidative stress\u2010mediated gut microbiota disorder and colitis. The research further clarified the mechanism of melatonin in improving colitis from the perspective of the interaction between <jats:italic>Aeromonas<\/jats:italic> and goblet cells. <jats:italic>Methods<\/jats:italic>. A seventy\u2010two hours SD mouse model with or without melatonin intervention and fecal microbiota transplantation (FMT) to explore the vital position of <jats:italic>Aeromonas<\/jats:italic>\u2010goblet cell interactions in melatonin improving SD\u2010induced colitis. Moreover, <jats:italic>Aeromonas<\/jats:italic> or LPS\u2010supplied mice were assessed, and the influence of melatonin on <jats:italic>Aeromonas<\/jats:italic>\u2010goblet cell interactions\u2010mediated oxidative stress caused colitis. Furthermore, in vitro experiment investigated the regulation mechanism of melatonin.<jats:italic>Results<\/jats:italic>. Our study showed that SD induced colitis, with upregulation of <jats:italic>Aeromonas<\/jats:italic> and LPS levels and reductions in goblet cells number and MUC2 protein. Similarly, FMT from SD mice, <jats:italic>Aeromonas<\/jats:italic> veronii colonization, and LPS treatment restored the SD\u2010like goblet cells number and MUC2 protein decrease and colitis. Moreover, LPS treatment downregulated the colonic antioxidant capacity. Yet, melatonin intervention reversed all consequence in SD, <jats:italic>A.<\/jats:italic>veronii colonization, and LPS\u2010treated mice. In vitro, melatonin reversed <jats:italic>A.<\/jats:italic> veronii\u2010 or LPS\u2010induced MUC2 depletion in mucus\u2010secreting human HT\u201029 cells via increasing the expression level of Villin, Tff3, p\u2010GSK\u20103<jats:italic>\u03b2<\/jats:italic>, <jats:italic>\u03b2<\/jats:italic>\u2010catenin, and melatonin receptor 2 (MT2) and decreasing the level of p\u2010I<jats:italic>\u03ba<\/jats:italic>B, p\u2010P65, ROS, TLR4, and MyD88 proteins, while the improvement effect was blocked with pretreatment with a MT2 antagonist but were mimicked by TLR4 and GSK\u20103<jats:italic>\u03b2<\/jats:italic> antagonists and ROS scavengers. <jats:italic>Conclusions<\/jats:italic>. Our results demonstrated that melatonin\u2010mediated MT2 inhibits <jats:italic>Aeromonas<\/jats:italic>\u2010goblet cell interactions to restore the level of MUC2 production via LPS\/TLR4\/MyD88\/GSK\u20103<jats:italic>\u03b2<\/jats:italic>\/ROS\/NF\u2010<jats:italic>\u03ba<\/jats:italic>B loop, further improving colitis in SD mice.<\/jats:p>","DOI":"10.1155\/2022\/8133310","type":"journal-article","created":{"date-parts":[[2022,3,21]],"date-time":"2022-03-21T03:16:27Z","timestamp":1647832587000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["The Role of <i>Aeromonas<\/i>\u2010Goblet Cell Interactions in Melatonin\u2010Mediated Improvements in Sleep Deprivation\u2010Induced Colitis"],"prefix":"10.1155","volume":"2022","author":[{"given":"Ting","family":"Gao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zixu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yulan","family":"Dong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2576-5977","authenticated-orcid":false,"given":"Yaoxing","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,3,20]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/9981480"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2021.107779"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222111894"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/ibd\/izy334"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1586\/eci.10.83"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2014-308323"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms15069594"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1237910"},{"key":"e_1_2_9_9_2","first-page":"211","volume-title":"The Epithelial Mucins: Structure\/Function Roles in Cancer and Inflammatory Diseases","author":"Renes I. 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