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The present study aimed to investigate the chemopreventive effects of water-soluble polysaccharides from Ganoderma resinaceum on HCC. Two G. resinaceum polysaccharide-rich fractions (GRP I and GRP II) were obtained following hot water and alcohol precipitation. Their proteins, phenol compounds, and total neutral sugar content were assayed. The in vitro antiproliferative effect was assessed in MDA-MB 231, Hela, and HepG2 using the MTT assay. Further, for the in vivo study, seven groups of nine rats each received N-diethylnitrosamine (100\u2009mg\/kg BW), vehicle (NaCl 0.9%), doxorubicin (10\u2009mg\/kg BW), or G. resinaceum polysaccharides (125 and 250\u2009mg\/kg BW). Liver cancer initiation and progression was assessed by evaluating histomorphology of liver section, hepatic injury markers, hematology, cytokines\/chemokines levels, and stress oxidative markers. GRP II presented higher protein and sugar and lower phenol compound content than GRP I. GRP exhibited CC50 of 340 and 261.7 in HepG2 cells after 48\u2009h. Moreover, GRP I and GRP II (125 and 250\u2009mg\/kg) prevented the alteration of the histoarchitecture of the liver induced by the DEN. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alpha-fetoprotein (AFP), proinflammatory cytokines (G-CSF, IFN\u03b3, and TNF\u03b1), and chemokines (eotaxin and fractalkine) levels were significantly decreased in the GRP I- and GRP II-treated groups, while anti-inflammatory cytokines (IL-10 and IL-12p70) levels were increased. The antioxidant defense was also stimulated by reducing malondialdehyde (MDA) and nitric oxide (NO2) levels, increasing catalase (CAT) and superoxide dismutase (SOD) activities, and reducing glutathione (GSH) levels. Our results indicate that GRP I exhibits chemopreventive effects by inhibiting cell proliferation and restoring liver architecture, antioxidant enzymes, and cytokines\/chemokines balance.<\/jats:p>","DOI":"10.1155\/2022\/8198859","type":"journal-article","created":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T18:26:07Z","timestamp":1649787967000},"page":"1-15","source":"Crossref","is-referenced-by-count":12,"title":["Polysaccharide-Rich Fractions from Ganoderma resinaceum (Ganodermataceae) as Chemopreventive Agents in N-Diethylnitrosamine-Induced Hepatocellular Carcinoma in Wistar Rats"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2386-3020","authenticated-orcid":true,"given":"Marius Tr\u00e9sor Kemegne","family":"Sipping","sequence":"first","affiliation":[{"name":"Laboratory of Phytoprotection and Valorization of Genetic Resources, Biotechnology Centre, Nkolbisson, University of Yaound\u00e9 1, P.O. Box 17673, Etetak, Yaound\u00e9, Cameroon"},{"name":"Department of Animal Biology and Physiology, Faculty of Science, University of Yaound\u00e9 1, P.O. Box 812, Yaound\u00e9, Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7191-4876","authenticated-orcid":true,"given":"Francine Kengne","family":"Mediesse","sequence":"additional","affiliation":[{"name":"Laboratory of Phytoprotection and Valorization of Genetic Resources, Biotechnology Centre, Nkolbisson, University of Yaound\u00e9 1, P.O. Box 17673, Etetak, Yaound\u00e9, Cameroon"},{"name":"Centre for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plant Studies, P.O. Box 13033, Yaound\u00e9, Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3556-5541","authenticated-orcid":true,"given":"Larissa V.","family":"Kenmogne","sequence":"additional","affiliation":[{"name":"Laboratory of Phytoprotection and Valorization of Genetic Resources, Biotechnology Centre, Nkolbisson, University of Yaound\u00e9 1, P.O. Box 17673, Etetak, Yaound\u00e9, Cameroon"},{"name":"Department of Surgery, Faculty of Health Sciences, University of Witwatersrand, Johannesburg 2193, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8648-5026","authenticated-orcid":true,"given":"Judith Emery Ngomoyogoli","family":"Kanemoto","sequence":"additional","affiliation":[{"name":"Laboratory of Phytoprotection and Valorization of Genetic Resources, Biotechnology Centre, Nkolbisson, University of Yaound\u00e9 1, P.O. Box 17673, Etetak, Yaound\u00e9, Cameroon"},{"name":"Genomics Laboratory, Department of Biological Sciences, Birla Institute of Technology & Science, Pilani, Hyderabad Campus, Jawahar Nagar, Shamirpet Mandal, Hyderabad, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1358-8221","authenticated-orcid":true,"given":"Dieudonn\u00e9","family":"Njamen","sequence":"additional","affiliation":[{"name":"Department of Animal Biology and Physiology, Faculty of Science, University of Yaound\u00e9 1, P.O. Box 812, Yaound\u00e9, Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1205-4255","authenticated-orcid":true,"given":"Thadd\u00e9e","family":"Boudjeko","sequence":"additional","affiliation":[{"name":"Laboratory of Phytoprotection and Valorization of Genetic Resources, Biotechnology Centre, Nkolbisson, University of Yaound\u00e9 1, P.O. 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