{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T02:14:56Z","timestamp":1791684896374,"version":"4.3.4"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,10,5]],"date-time":"2022-10-05T00:00:00Z","timestamp":1664928000000},"content-version":"vor","delay-in-days":277,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. The emergence of resistance to beta\u2010lactam agents in poultry results in multidrug\u2010resistant (MDR) phenotypes in <jats:italic>Escherichia coli<\/jats:italic> isolates from poultry birds. The appearance of mobile colistin resistance (<jats:italic>mcr<\/jats:italic>) genes in the poultry sector has further worsened the situation. Therefore, the current study is aimed at investigating the molecular epidemiology of <jats:italic>mcr<\/jats:italic> harboring colistin\u2010resistant <jats:italic>E. coli<\/jats:italic> among poultry. <jats:italic>Methods<\/jats:italic>. The isolation and identification of colistin\u2010resistant <jats:italic>E. coli<\/jats:italic> (CR\u2010Ec) were done from the broiler\u2019s fecal samples through culturing using selective media supplemented with colistin sulfate (4\u2009<jats:italic>\u03bc<\/jats:italic>g\/ml). The antibiogram studies of the isolates were performed using the disc diffusion method and broth microdilution method as per CLSI guidelines. The screening for the genes conferring resistance to colistin as well as beta\u2010lactam agents was performed by PCR. The genetic diversity of <jats:italic>mcr-<\/jats:italic>positive strains was assessed by multilocus sequencing typing (MLST). <jats:italic>Results<\/jats:italic>. Out of 500 fecal samples, 7% (35\/500) were found positive for the presence of colistin\u2010resistant <jats:italic>E. coli<\/jats:italic> (CR\u2010Ec). Among the CR\u2010Ec isolates, 74.28% (26\/35) were detected as ESBL producers carrying the <jats:italic>bla<\/jats:italic>CTX\u2010M\u20101 gene in 15\/35 (42.85%) isolates and <jats:italic>bla<\/jats:italic>CTX\u2010M\u201015 and <jats:italic>bla<\/jats:italic>TEM genes in 21\/35 (60%) and 35\/35 (100%) isolates, respectively. <jats:italic>E. coli<\/jats:italic> isolates were found positive for the presence of <jats:italic>mcr<\/jats:italic>\u20101, although none of the isolates exhibited the <jats:italic>mcr<\/jats:italic>\u20102 or <jats:italic>mcr<\/jats:italic>\u20103 genes. The MLST of CR\u2010Ec has shown the ST1035 as the most prevalent genotype, while 82.85% (29\/35) of CR\u2010Ec strains belonged to clonal complex (CC) 131 comprising ST1035, ST131, ST1215, ST1650, and ST2279. <jats:italic>Conclusions<\/jats:italic>. The findings suggest a continuous monitoring system in veterinary and clinical settings to avoid unnecessary antibiotics. Further studies are needed at the national level to help control the increasing resistance among Enterobacterales in poultry settings.<\/jats:p>","DOI":"10.1155\/2022\/8224883","type":"journal-article","created":{"date-parts":[[2022,10,5]],"date-time":"2022-10-05T16:06:00Z","timestamp":1664985960000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Genetic Diversity of <i>Escherichia coli<\/i> Coharboring <i>mcr<\/i>\u20101 and Extended Spectrum Beta\u2010Lactamases from Poultry"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6828-3295","authenticated-orcid":false,"given":"Arslan","family":"Jamil","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0895-7312","authenticated-orcid":false,"given":"Muhammad Asif","family":"Zahoor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4673-2579","authenticated-orcid":false,"given":"Zeeshan","family":"Nawaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8589-2292","authenticated-orcid":false,"given":"Abu Baker","family":"Siddique","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3196-2857","authenticated-orcid":false,"given":"Mohsin","family":"Khurshid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,10,5]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12941-018-0255-z"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0180599"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.13222"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S173867"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a025288"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.12049"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.03.009"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-021-05906-1"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S209473"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkw411"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2021.126881"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X.JFP-13-510"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2016.07.001"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01945-16"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2020.108766"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/jam.15469"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S302687"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S296219"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.771510"},{"key":"e_1_2_8_20_2","first-page":"78","article-title":"Hematobiochemical effects of colistin administered intramuscularly in growing broiler birds","volume":"34","author":"Saleemi M. 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