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Herein, we aimed to explore the effect of glibenclamide in vivo and in vitro on the development of LPS-induced ALI in a mouse model. LPS stimulation resulted in increases in lung injury score, wet\/dry ratio, and capillary permeability in lungs, as well as in total protein concentration, inflammatory cells, and inflammatory cytokines including IL-1\u03b2, IL-18 in bronchoalveolar lavage fluid (BALF), and lung tissues, whereas glibenclamide treatment reduced these changes. Meanwhile, the increased proteins of NLRP3 and Caspase-1\/p20 after LPS instillation in lungs were downregulated by glibenclamide. Similarly, in vitro experiments also found that glibenclamide administration inhibited the LPS-induced upregulations in cytokine secretions of IL-1\u03b2 and IL-18, as well as in the expression of components in NLRP3 inflammasome in mouse peritoneal macrophages. Of note, glibenclamide had no effect on the secretion of TNF-\u03b1 in vivo nor in vitro, implicating that its anti-inflammatory effect is relatively specific to NLRP3 inflammasome. In conclusion, glibenclamide alleviates the development of LPS-induced ALI in a mouse model via inhibiting the NLRP3\/Caspase-1\/IL-1\u03b2 signaling pathway, which might provide a new strategy for the treatment of LPS-induced ALI.<\/jats:p>","DOI":"10.1155\/2022\/8457010","type":"journal-article","created":{"date-parts":[[2022,2,11]],"date-time":"2022-02-11T22:20:21Z","timestamp":1644618021000},"page":"1-12","source":"Crossref","is-referenced-by-count":21,"title":["Glibenclamide Alleviates LPS-Induced Acute Lung Injury through NLRP3 Inflammasome Signaling Pathway"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9200-9664","authenticated-orcid":true,"given":"Jie","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Critical Care Medicine, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6584-232X","authenticated-orcid":true,"given":"Jiawen","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Immunology, and Department of Orthopaedics of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5292-0250","authenticated-orcid":true,"given":"Xiaofang","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Critical Care Medicine, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China"},{"name":"Department of Critical Care Medicine, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4641-7525","authenticated-orcid":true,"given":"Huiqing","family":"Xiu","sequence":"additional","affiliation":[{"name":"Department of Critical Care Medicine, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8131-6979","authenticated-orcid":true,"given":"Songjie","family":"Bai","sequence":"additional","affiliation":[{"name":"Department of Critical Care Medicine, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2619-6668","authenticated-orcid":true,"given":"Jiahui","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Critical Care Medicine, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4317-2630","authenticated-orcid":true,"given":"Zhijian","family":"Cai","sequence":"additional","affiliation":[{"name":"Institute of Immunology, and Department of Orthopaedics of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6375-8797","authenticated-orcid":true,"given":"Zhanghui","family":"Chen","sequence":"additional","affiliation":[{"name":"Zhanjiang Institute of Clinical Medicine, Zhanjiang Central Hospital, Guangdong Medical University, Zhanjiang 524045, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1842-1367","authenticated-orcid":true,"given":"Shufang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9298-3961","authenticated-orcid":true,"given":"Gensheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Critical Care Medicine, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China"},{"name":"Key Laboratory of the Diagnosis and Treatment of Severe Trauma and Burn of Zhejiang Province, China"},{"name":"Zhejiang Province Clinical Research Center for Emergency and Critical Care Medicine, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"issue":"1","key":"1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1097\/MCC.0000000000000057","article-title":"The acute respiratory distress syndrome: incidence and mortality, has it changed?","volume":"20","author":"J. 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