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Plasticity"],"published-print":{"date-parts":[[2022,7,11]]},"abstract":"<jats:p>Temporal lobe epilepsy (TLE) is a chronic neurological disorder that is often resistant to antiepileptic drugs. The pathogenesis of TLE is extremely complicated and remains elusive. Understanding the molecular mechanisms underlying TLE is crucial for its diagnosis and treatment. In the present study, a lithium-pilocarpine-induced TLE model was employed to reveal the pathological changes of hippocampus in rats. Hippocampal samples were taken for proteomic analysis at 2 weeks after the onset of spontaneous seizure (a chronic stage of epileptogenesis). Isobaric tag for relative and absolute quantization (iTRAQ) coupled with liquid chromatography-tandem mass spectrometry (LC\u2013MS\/MS) technique was applied for proteomic analysis of hippocampus. A total of 4173 proteins were identified from the hippocampi of epileptic rats and its control, of which 27 differentially expressed proteins (DEPs) were obtained with a <jats:inline-formula>\n                     <a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                        <a:mtext>fold<\/a:mtext>\n                        <a:mtext>\u2009<\/a:mtext>\n                        <a:mtext>change<\/a:mtext>\n                        <a:mo>&gt;<\/a:mo>\n                        <a:mn>1.5<\/a:mn>\n                     <\/a:math>\n                  <\/jats:inline-formula> and <jats:inline-formula>\n                     <c:math xmlns:c=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\">\n                        <c:mi>P<\/c:mi>\n                        <c:mo>&lt;<\/c:mo>\n                        <c:mn>0.05<\/c:mn>\n                     <\/c:math>\n                  <\/jats:inline-formula>. Bioinformatics analysis indicated 27 DEPs were mainly enriched in \u201cregulation of synaptic plasticity and structure\u201d and \u201ccalmodulin-dependent protein kinase activity,\u201d which implicate synaptic remodeling may play a vital role in the pathogenesis of TLE. Consequently, the synaptic plasticity-related proteins and synaptic structure were investigated to verify it. It has been demonstrated that CaMKII-\u03b1, CaMKII-\u03b2, and GFAP were significant upregulated coincidently with proteomic analysis in the hippocampus of TLE rats. Moreover, the increased dendritic spines and hippocampal sclerosis further proved that synaptic plasticity involves in the development of TLE. The present study may help to understand the molecular mechanisms underlying epileptogenesis and provide a basis for further studies on synaptic plasticity in TLE.<\/jats:p>","DOI":"10.1155\/2022\/8511066","type":"journal-article","created":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T00:20:09Z","timestamp":1657585209000},"page":"1-12","source":"Crossref","is-referenced-by-count":18,"title":["Proteomic Analysis Reveals the Vital Role of Synaptic Plasticity in the Pathogenesis of Temporal Lobe Epilepsy"],"prefix":"10.1155","volume":"2022","author":[{"given":"Xu","family":"Qian","sequence":"first","affiliation":[{"name":"Department of Clinical Pharmacology, Key Laboratory of Molecular Target & Clinical Pharmacology, School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou, Guangdong 511436, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji-Qiang","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, 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