{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T12:31:40Z","timestamp":1774701100757,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2022,4,28]],"date-time":"2022-04-28T00:00:00Z","timestamp":1651104000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82002407"],"award-info":[{"award-number":["82002407"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2022,4,28]]},"abstract":"<jats:p>Background. Neoadjuvant immunotherapy is promising for locally advanced non-small-cell lung cancer (NSCLC). The in situ immune patterns, as a predictor of PD-1\/PD-L1 blockade outcomes, of the primary tumor (PT) and metastatic lymph nodes (mLNs) are unknown. Methods. Multiplex immunofluorescence staining and multispectral imaging were used to evaluate the in situ immune patterns of T cells (CD3+) and cytotoxic T cells (CD8+) in terms of density, location (center of tumor (CT) and invasive margin (IM)), and the PD-L1 expression status of tumor cells and stromal T cells of paired PTs and mLNs in 38 stage III NSCLCs. Results. The densities of T cells and cytotoxic T cells were correlated between PTs and mLNs at both CT and IM. Higher densities of stromal T cells (S-CD3+) at CT and both S-CD3+ and cytotoxic T cells (S-CD8+) at IM were observed in mLNs compared to PTs, while in tumor compartment, there were no differences in the densities of T cells (T-CD3+) or cytotoxic T cells (T-CD8+). Only the density of stromal PD-L1-positive T cells (S-PD-L1+CD3+) at CT was correlated between PTs and mLNs, while the densities and frequencies of S-PD-L1+CD3+ at CT and IM of mLNs were higher than PTs. Combining positive score discordance of PD-L1 between PTs and mLNs was greater than tumor proportion score. Conclusions. In situ immune patterns of T cells and cytotoxic T cells were different between PTs and mLNs in NSCLC. The heterogeneity of the in situ immune patterns may result in different immune-mediated responses to neoadjuvant immunotherapy in PT and mLNs.<\/jats:p>","DOI":"10.1155\/2022\/8513747","type":"journal-article","created":{"date-parts":[[2022,4,29]],"date-time":"2022-04-29T01:07:02Z","timestamp":1651194422000},"page":"1-14","source":"Crossref","is-referenced-by-count":3,"title":["Different In Situ Immune Patterns between Primary Tumor and Lymph Node in Non-Small-Cell Lung Cancer: Potential Impact on Neoadjuvant Immunotherapy"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8588-0331","authenticated-orcid":true,"given":"Zheng-Hao","family":"Ye","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oncology in Southern China, Collaborative Innovation Center for Cancer Medicine, and Department of Thoracic Surgery, Sun Yat-Sen University Cancer Center, Guangzhou, China"},{"name":"Lung Cancer Research Center, Sun Yat-Sen University, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5623-9799","authenticated-orcid":true,"given":"Hao","family":"Long","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oncology in Southern China, Collaborative Innovation Center for Cancer Medicine, and Department of Thoracic Surgery, Sun Yat-Sen University Cancer Center, Guangzhou, China"},{"name":"Lung Cancer Research Center, Sun Yat-Sen University, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9911-9965","authenticated-orcid":true,"given":"Ze-Rui","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oncology in Southern China, Collaborative Innovation Center for Cancer Medicine, and Department of Thoracic Surgery, Sun Yat-Sen University Cancer Center, Guangzhou, China"},{"name":"Lung Cancer Research Center, Sun Yat-Sen University, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21551"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)32409-7"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1507643"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1716948"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1504627"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)32517-X"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2017.101"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3245"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2013.03.004"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-11-0268"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-10-1"},{"key":"12","doi-asserted-by":"crossref","first-page":"5944","DOI":"10.1200\/JCO.2008.19.6147","article-title":"In situ cytotoxic and memory T cells predict outcome in patients with early-stage colorectal cancer","volume":"27","author":"F. 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