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Guillain\u2013Barr\u00e9 syndrome (GBS) is a common autoimmune disease of the peripheral nervous system, and there is still no effective treatment for GBS. This investigation intends to figure out the effect and mechanism of N-type voltage-gated calcium (Cav2.2) channels on neuropathic pain in GBS. Methods. An experimental autoimmune neuritis (EAN) model was established in Lewis rats induced by myelin P253-78 peptide and complete Freund\u2019s adjuvant. Luxol fast blue (LFB) staining was used for observing the degree of cell infiltration and demyelination in the sciatic nerve of rats, ELISA for detecting IL-6 and TNF-\u03b1 expression in the serum, qRT-PCR, and Western blot for measuring the expression of iNOS, MCP-1, and Cav2.2 in the sciatic nerve, respectively. Results. EAN led to significant decreases in the mechanical withdrawal threshold, thermal withdrawal threshold, and mechanical hyperalgesia threshold and an increase in the withdrawal threshold to cold stimulation. The serum IL-6 and TNF-\u03b1 expression was significantly increased, and the mRNA and protein expression of iNOS, MCP-1, and Cav2.2 in the sciatic nerve were significantly increased in the EAN rats. However, silencing Cav2.2 expression could significantly reverse the above EAN-caused results. Conclusion. Silencing Cav2.2 expression can significantly reduce the clinical score, pathological injury, and mechanical allodynia, reducing the release of inflammatory factors, thus improving neuropathic pain in EAN rats.<\/jats:p>","DOI":"10.1155\/2022\/8547095","type":"journal-article","created":{"date-parts":[[2022,6,15]],"date-time":"2022-06-15T19:21:34Z","timestamp":1655320894000},"page":"1-7","source":"Crossref","is-referenced-by-count":3,"title":["Upregulation of N-Type Voltage-Gated Calcium Channels Induces Neuropathic Pain in Experimental Autoimmune Neuritis"],"prefix":"10.1155","volume":"2022","author":[{"given":"Mei","family":"Mao","sequence":"first","affiliation":[{"name":"Department of Neurology, Guizhou Provincial People\u2019s Hospital, Guiyang, Guizhou 550002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Neurology, Guizhou Provincial People\u2019s Hospital, Guiyang, Guizhou 550002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Neurology, Guizhou Provincial People\u2019s Hospital, Guiyang, Guizhou 550002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pan","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Neurology, Guizhou Provincial People\u2019s Hospital, Guiyang, Guizhou 550002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Xi","sequence":"additional","affiliation":[{"name":"Department of Neurology, Guizhou Provincial People\u2019s Hospital, Guiyang, Guizhou 550002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3594-1180","authenticated-orcid":true,"given":"Yuanrong","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Neurology, Guizhou Provincial People\u2019s Hospital, Guiyang, Guizhou 550002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(16)00339-1"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/s1474-4422(08)70215-1"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1056\/nejmra1114525"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1038\/nrneurol.2014.121"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awm004"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-010-5836-5"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1038\/268752a0"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/121.10.1895"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510x(87)90235-8"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1084\/jem.102.2.213"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2017.00302"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/s0006-3495(81)84899-0"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1038\/srep00366"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.4161\/chan.3.4.9255"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1038\/nn1605"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02132"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2018.11.008"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2019.03.009"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.21769\/bioprotoc.2506"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1055\/s-0039-1693005"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-013-8388-5"},{"key":"22","first-page":"389","article-title":"Inflammatory infiltration of sciatic nerves in rat EAN models at the chronic phase","volume-title":"Chinese Journal of Neuroimmunology and Neurology","author":"X. 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