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At present, Ling-Gui-Zhu-Gan decoction (LGZGD) has been widely reported in the clinical treatment and basic research of cardiovascular diseases (CVDs), such as myocardial infarction, heart failure, and angina pectoris. However, the mechanism of LGZGD against VR after AMI remains unclear. Ultra-performance liquid chromatography (UPLC) was applied to investigate the major constituents of LGZGD, and molecular docking was used to predict the targets on the NLRP3\/Caspase-1\/GSDMD signaling pathway. In vivo, histological changes in the myocardium were visualized using HE staining and Masson staining, and cardiomyocyte apoptosis was detected using TUNEL. IL-1\u03b2 activity in rat serum was determined by ELISA. Finally, NLRP3, Caspase-1, and GSDMD expressions were analyzed through RT-qPCR and Western blotting. The results showed that 8 authentic reference substances have been detected in LGZGD. Molecular docking showed that the major chemical constituents of LGZGD had a good binding activity with NLRP3, Caspase-1, and GSDMD. Our results showed that LGZGD treatment markedly improved cardiac pathology, decreased cardiomyocyte apoptosis, reduced IL-1\u03b2 activity, and regulated the expression of genes and proteins related to the NLRP3\/Caspase-1\/GSDMD signal pathway. These results suggest that LGZGD protects against VR after AMI through NLRP3\/Caspase-1\/GSDMD signal pathway.<\/jats:p>","DOI":"10.1155\/2022\/8593176","type":"journal-article","created":{"date-parts":[[2022,5,16]],"date-time":"2022-05-16T21:05:08Z","timestamp":1652735108000},"page":"1-14","source":"Crossref","is-referenced-by-count":4,"title":["Exploring the Mechanism of Ling-Gui-Zhu-Gan Decoction in Ventricular Remodeling after Acute Myocardial Infarction Based on UPLC and In Vivo Experiments"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7322-692X","authenticated-orcid":true,"given":"Peng","family":"Zhou","sequence":"first","affiliation":[{"name":"School of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei 230012, China"},{"name":"Institute of Integrated Chinese and Western Medicine, Anhui Academy of Chinese Medicine, Hefei 230012, China"},{"name":"Anhui Province Key Laboratory of Chinese Medicinal Formula, 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