{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T23:22:49Z","timestamp":1788909769425,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T00:00:00Z","timestamp":1644969600000},"content-version":"vor","delay-in-days":46,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81960231"],"award-info":[{"award-number":["81960231"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p>Ischemic stroke is a common disease of the central nervous system, and ischemic brain injury (IBI) is its main manifestation. Recently, extracellular vesicles (EVs) have been strongly related to the diagnosis and treatment of IBI. However, the underlying mechanism of their effects remains enigmatic. In the present study, we aimed to study how miR\u2010155\u20105p plays a role in choroid plexus epithelial (CPE) cell\u2010derived EVs in IBI pathology. We found that miR\u2010155\u20105p expression was enriched in CPE cell\u2010derived EVs, which were subsequently internalized by neurons, enabling the delivery of miR\u2010155\u20105p into neurons. An inducible oxygen and glucose deprivation and reoxygenation (OGD\/R) cell model was developed to mimic ischemic neuronal injury <jats:italic>in vitro<\/jats:italic>. miR\u2010155\u20105p overexpression led to reduced neuron viability, promoted apoptosis, elevated autophagic proteins\u2019 expression, and activated NLR family pyrin domain\u2010containing 3\u2010 (NLRP3\u2010) related inflammasomes, thereby aggravating OGD\u2010induced neuronal injury. A dual\u2010luciferase reporter assay exhibited that miR\u2010155\u20105p could inhibit the Ras homolog enriched in brain (Rheb) expression, a mechanism critical for miR\u2010155\u20105p\u2010mediated neuronal injury. Furthermore, a mouse IBI model was developed using the transient middle cerebral artery occlusion (tMCAO) method. Animal experiments verified that miR\u2010155p delivery via CPE cell\u2010derived EVs aggravated IBI by suppressing Rheb expression. In conclusion, miR\u2010155\u20105p in CPE\u2010derived EVs can aggravate IBI pathology by suppressing Rheb expression and promoting NLRP3\u2010mediated inflammasomes, suggesting its role as a potential therapeutic target in IBI.<\/jats:p>","DOI":"10.1155\/2022\/8603427","type":"journal-article","created":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T20:35:18Z","timestamp":1645043718000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["miR\u2010155\u20105p in Extracellular Vesicles Derived from Choroid Plexus Epithelial Cells Promotes Autophagy and Inflammation to Aggravate Ischemic Brain Injury in Mice"],"prefix":"10.1155","volume":"2022","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2797-7535","authenticated-orcid":false,"given":"Zhang","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofang","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zidan","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1004-0300","authenticated-orcid":false,"given":"Lan","family":"Chu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,2,16]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(21)00252-0"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2020.11.002"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.7150\/thno.47651"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.618663"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2951"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2019.03.004"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.116.024807"},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aau8301"},{"key":"e_1_2_11_9_2","doi-asserted-by":"publisher","DOI":"10.5966\/sctm.2015-0197"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00280.2019"},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/MOT.0000000000000701"},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.201606271"},{"key":"e_1_2_11_13_2","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2018.9236"},{"key":"e_1_2_11_14_2","doi-asserted-by":"publisher","DOI":"10.2174\/1567202615666180601081409"},{"key":"e_1_2_11_15_2","doi-asserted-by":"publisher","DOI":"10.26355\/eurrev_202002_20198"},{"key":"e_1_2_11_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.24101"},{"key":"e_1_2_11_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.02.004"},{"key":"e_1_2_11_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-2539-4"},{"key":"e_1_2_11_19_2","article-title":"Diosgenin glucoside protects against spinal cord injury by regulating autophagy and alleviating apoptosis","volume":"19","author":"Chen X. 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